Brain Endothelial Cells Activate Neuroinflammatory Pathways in Response to Early Cerebral Small Vessel Disease (CSVD) Patients’ Plasma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients’ Sample Selection Process
2.2. Human Brain Endothelial Cells Culture and Treatments
2.3. RNA Isolation, Library Preparation and Next Generation Sequencing (NGS)
2.4. RNA-Seq Bioinformatics Analysis
2.5. Target Gene Expression Analysis
2.6. Statistical Analysis
3. Results
3.1. Treatments with Plasma
3.2. Study of the Effects of CSVD Plasma Incubation on Molecular Expression in HBEC5i Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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GO Term | Description |
---|---|
GO:0036500 | ATF6-mediated unfolded protein response |
GO:0007050 | cell cycle arrest |
GO:0070887 | cellular response to chemical stimulus |
GO:0071310 | cellular response to organic substance |
GO:0051716 | cellular response to stimulus |
GO:0033554 | cellular response to stress |
GO:0019221 | cytokine-mediated signaling pathway |
GO:0001892 | embryonic placenta development |
GO:0030968 | endoplasmic reticulum unfolded protein response |
GO:0043066 | negative regulation of apoptotic process |
GO:0010648 | negative regulation of cell communication |
GO:0060548 | negative regulation of cell death |
GO:0043069 | negative regulation of programmed cell death |
GO:0048585 | negative regulation of response to stimulus |
GO:0023057 | negative regulation of signaling |
GO:1901564 | organonitrogen compound metabolic process |
GO:1990440 | positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress |
GO:0036003 | positive regulation of transcription from RNA polymerase II promoter in response to stress |
GO:0042981 | regulation of apoptotic process |
GO:0010646 | regulation of cell communication |
GO:0051726 | regulation of cell cycle |
GO:0031326 | regulation of cellular biosynthetic process |
GO:0080135 | regulation of cellular response to stress |
GO:0043067 | regulation of programmed cell death |
GO:0031399 | regulation of protein modification process |
GO:1905897 | regulation of response to endoplasmic reticulum stress |
GO:0080134 | regulation of response to stress |
GO:0023051 | regulation of signaling |
GO:0042762 | regulation of sulfur metabolic process |
GO:1901342 | regulation of vasculature development |
GO:0042221 | response to chemical |
GO:0034976 | response to endoplasmic reticulum stress |
GO:0010033 | response to organic substance |
GO:0006950 | response to stress |
GO:0035966 | response to topologically incorrect protein |
GO:0006986 | response to unfolded protein |
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Cifù, A.; Janes, F.; Mio, C.; Domenis, R.; Pessa, M.E.; Garbo, R.; Curcio, F.; Valente, M.; Fabris, M. Brain Endothelial Cells Activate Neuroinflammatory Pathways in Response to Early Cerebral Small Vessel Disease (CSVD) Patients’ Plasma. Biomedicines 2023, 11, 3055. https://doi.org/10.3390/biomedicines11113055
Cifù A, Janes F, Mio C, Domenis R, Pessa ME, Garbo R, Curcio F, Valente M, Fabris M. Brain Endothelial Cells Activate Neuroinflammatory Pathways in Response to Early Cerebral Small Vessel Disease (CSVD) Patients’ Plasma. Biomedicines. 2023; 11(11):3055. https://doi.org/10.3390/biomedicines11113055
Chicago/Turabian StyleCifù, Adriana, Francesco Janes, Catia Mio, Rossana Domenis, Maria Elena Pessa, Riccardo Garbo, Francesco Curcio, Mariarosaria Valente, and Martina Fabris. 2023. "Brain Endothelial Cells Activate Neuroinflammatory Pathways in Response to Early Cerebral Small Vessel Disease (CSVD) Patients’ Plasma" Biomedicines 11, no. 11: 3055. https://doi.org/10.3390/biomedicines11113055