Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood
Round 1
Reviewer 1 Report
in the attached file
Comments for author File: Comments.pdf
Author Response
See attached PDF
Author Response File: Author Response.pdf
Reviewer 2 Report
The topic of the results article is important from the point of view of setting standards for further research and comparability of published results.
I have several comments on the evaluated manuscript.
Abstract:
The article evaluates the stability of proteins, however, the abstract does not contain the word protein even once. Please include it in the appropriate place of the abstract.
The abstract should contain the number of tested samples or biological replicates. In short, information about the size or scope of testing.
Line 27-28 Sample values - specify the units
Text of the manuscript:
Line 43 Provide a citation.
Line 44 Add at least one additional citation.
Line 48 PD markers - describe the abbreviation
Line 67 a variety of clinically --- add: short-term stability, freeze effects, refreeze effects and sample preparation before application
Line 88 low, medium and high concentration - please specify
Line 280 CLSI-guideline - describe the abbreviation
Line 291 acceptable
acceptable recoveries for PD-1 between 80 and 120% - presented only in the abstract, state here or in a more appropriate place in the text, what is acceptable
Author Response
See attached PDF
Author Response File: Author Response.pdf