Aminopeptidase Activities Interact Asymmetrically between Brain, Plasma and Systolic Blood Pressure in Hypertensive Rats Unilaterally Depleted of Dopamine
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Groups
2.2. 6-Hydroxydopamine and Saline Intrastriatal Injections
2.3. Obtaining Brain and Plasma Samples
2.4. Aminopeptidase Activities and Protein Determinations
2.5. Statistical Analysis
3. Results
3.1. Systolic Blood Pressure in Hypertensive Rats
3.2. Aminopeptidase Activities in Brain and Plasma
3.3. Sham Left Correlations in Spontaneously Hypertensive Rats
3.4. Sham Right Correlations in Spontaneously Hypertensive Rats
3.5. Lesioned Left Correlations in Spontaneously Hypertensive Rats
3.6. Lesioned Right Correlations in Spontaneously Hypertensive Rats
3.7. Comparisons between Groups of Spontaneously Hypertensive Rats
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sham Left SHR | ||
Intra-Hemispheric | Inter-Hemispheric | |
Sham Left (Left vs. Left) | Sham Left (Right vs. Right) | Sham Left (Left vs. Right) |
LPAla vs. LPCys r = +0.869 p = 0.001 RPAla vs. RPAsp r = +0.815 p = 0.004 LPCys vs. LPGlu r = +0.658 p = 0.03 RPAsp vs. RPCys r = +0.789 p = 0.006 LPAsp vs. RPAsp r = +0.699 p = 0.02 RACys vs. RAGlu r = +0.872 p = 0.001 LAAla vs. LAGlu r = +0.922 p = 0.0001 RHAsp vs. RHCys r = −0.838 p = 0.002 LAAsp vs. LAGlu r = +0.916 p = 0.0002 RPAsp vs. RHAla r = +0.637 p = 0.04 LHAla vs. LHAsp r = +0.806 p = 0.004 LHAla vs. LHCys r = +0.744 p = 0.01 LHAsp vs. LHGlu r = +0.722 p = 0.01 | LPAla vs. LHCys r = +0.683 p = 0.02 LPAsp vs. LHAla r = +0.630 p = 0.05 LPAsp vs. LHCys r = +0.755 p = 0.01 LPAsp vs. LHGlu r = −0.794 p = 0.006 LPCys vs. LHCys r = +0.662 p = 0.03 LPGlu vs. LHCys r = +0.660 p = 0.03 | LPAsp vs. RPAla r = +0.614 p = 0.05 LPAsp vs. RPAsp r = +0.699 p = 0.02 LACys vs. RACys r = −0.628 p = 0.05 LPAla vs. RAGlu r = −0.649 p = 0.04 LHAla vs. RPAla r = +0.647 p = 0.04 LHAla vs. RACys r = −0.722 p = 0.01 LHAla vs. RACys r = −0.722 p = 0.01 LHAla vs. RAGlu r = −0.754 p = 0.01 LHAla vs. RAGlu r = −0.754 p = 0.01 LHCys vs. RAGlu r = −0.647 p = 0.04 |
Left Side vs. Plasma | Right Side vs. Plasma | Plasma vs. Plasma |
LACys vs. PlAla r = −0.615 p = 0.05 LHAsp vs. PlGlu r = +0.718 p = 0.01 LHCys vs. PlCys r = +0.678 p = 0.03 | RAAla vs. PlAsp r = −0.649 p = 0.04 RAGlu vs. PlCys r = −0.721 p = 0.01 RHGlu vs. PlGlu r = −0.703 p = 0.02 | No correlations |
Left Side vs. SBP | Right Side vs. SBP | |
No correlations | No correlations |
Sham Right SHR | ||
Intra-Hemispheric | Inter-Hemispheric | |
Sham Right (Left vs. Left) | Sham Right (Right vs. Right) | Sham Right (Left vs. Right) |
LAAla vs. LAGlu r = +0.757 p = 0.01 LAAsp vs. LAGlu r = +0.826 p = 0.003 LACys vs. LAGlu r = +0.674 p = 0.03 LHAla vs. LHCys r = +0.702 p = 0.02 LHCys vs. LHGlu r = +0.658 p = 0.03 LAAsp vs. LHAsp r = +0.927 p = 0.0001 LAGlu vs. LHAsp r = +0.747 p = 0.01 LPAsp vs. LHGlu r = −0.700 p = 0.02 LPGlu vs. LACys r = −0.780 p = 0.007 | RPAla vs. RPAsp r = +0.620 p = 0.05 RPAla vs. RPCys r = +0.889 p = 0.0006 RAAla vs. RAAsp r = +0.936 p = <0.0001 RAAla vs. RACys r = +0.920 p = 0.0002 RAAla vs. RAGlu r = +0.870 p = 0.001 RAAsp vs. RACys r = +0.908 p = 0.0003 RAAsp vs. RAGlu r = +0.842 p = 0.002 RACys vs. RAGlu r = +0.931 p = <0.0001 RPAsp vs. RHCys r = +0.836 p = 0.002 RPGlu vs. RAAla r = +0.718 p = 0.01 RPGlu vs. RAAsp r = +0.736 p = 0.01 RPGlu vs. RACys r = +0.681 p = 0.02 | LAAla vs. RAAla r = +0.638 p = 0.04 LAAsp vs. RAAla r = +0.917 p = 0.0002 LAAsp vs. RAAsp r = +0.852 p = 0.001 LAAsp vs. RACys r = +0.852 p = 0.001 LAAsp vs. RAGlu r = +0.902 p = 0.0004 LAGlu vs. RAAla r = +0.888 p = 0.0006 LAGlu vs. RAAsp r = +0.830 p = 0.002 LAGlu vs. RACys r = +0.762 p = 0.01 LAGlu vs. RAGlu r = +0.780 p = 0.007 LHAsp vs. RAAla r = +0.892 p = 0.005 LHAsp vs. RAAsp r = +0.850 p = 0.001 LHAsp vs. RACys r = +0.940 p = <0.0001 LHAsp vs. RAGlu r = +0.907 p = 0.0003 LHCys vs. RPAla r = +0.648 p = 0.04 LHGlu vs. RPAla r = +0.674 p = 0.03 LPGlu vs. RHGlu r = +0.679 p = 0.03 LAAla vs. RPGlu r = +0.775 p = 0.008 LPAla vs. RHAla r = −0.784 p = 0.007 LPAsp vs. RHAla r = −0.719 p = 0.01 |
Left Side vs. Plasma | Right Side vs. Plasma | Plasma vs. Plasma |
LPAla vs. PlAla r = +0.706 p = 0.02 LACys vs. PlGlu r = +0.703 p = 0.02 | RPGlu vs. PlAla r = +0.763 p = 0.01 RAAla vs. PlAla r = +0.660 p = 0.03 RAAsp vs. PlAla r = +0.811 p = 0.004 RACys vs. PlAla r = +0.678 p = 0.03 RPAsp vs. PlAsp r = +0.698 p = 0.02 RAAsp vs. PlCys r = +0.740 p = 0.01 RACys vs. PlCys r = +0.693 p = 0.02 RAGlu vs. PlCys r = +0.700 p = 0.02 | PlAla vs. PlCys r = +0.842 p = 0.002 |
Left Side vs. SBP | Right Side vs. SBP | |
No correlations | No correlations |
Lesioned left SHR | ||
Intra-Hemispheric | Inter-Hemispheric | |
Lesioned Left (Left vs. Left) | Lesioned Left (Right vs. Right) | Lesioned Left (Left vs. Right) |
LPAla vs. LPCys r = +0.674 p = 0.03 LPCys vs. LPGlu r = +0.689 p = 0.02 LHAla vs. LHAsp r = +0.923 p = 0.0001 LHAla vs. LHCys r = +0.975 p = <0.0001 LHAla vs. LHGlu r = +0.930 p = 0.0001 LHAsp vs. LHCys r = +0.856 p = 0.001 LHAsp vs. LHGlu r = +0.946 p = <0.0001 LHCys vs. LHGlu r = +0.901 p = 0.0004 LACys vs. LHAla r = −0.673 p = 0.03 LACys vs. LHAsp r = −0.646 p = 0.04 LACys vs. LHGlu r = −0.749 p = 0.01 | LPAla vs. LPCys r = +0.674 p = 0.03 RPAsp vs. RPCys r = +0.639 p = 0.04 RPAsp vs. RPGlu r = +0.616 p = 0.05 RPCys vs. RPGlu r = +0.808 p = 0.004 RHAla vs. RHCys r = +0.906 p = 0.0003 RPAla vs. RHAla r = +0.650 p = 0.04 RPAsp vs. RACys r = +0.782 p = 0.007 RAGlu vs. RHGlu r = −0.739 p = 0.01 | LPAsp vs. RPGlu r = +0.672 p = 0.03 LPGlu vs. RPAsp r = −0.629 p = 0.05 LPGlu vs. RPCys r = −0.636 p = 0.04 LAAla vs. RAAla r = +0.662 p = 0.03 LAAsp vs. RAGlu r = −0.703 p = 0.02 LAGlu vs. RAAsp r = +0.620 p = 0.05 LAGlu vs. RAGlu r = −0.658 p = 0.03 LPAsp vs. RAAsp r = −0.627 p = 0.05 LPCys vs. RACys r = −0.709 p = 0.02 LPGlu vs. RAAla r = −0.679 p = 0.03 LPGlu vsRACys r = −0.803 p = 0.005 LAAla vs. RPGlu r = +0.631 p = 0.05 LACys vs. RHAla r = +0.654 p = 0.04 LACys vs. RHCys r = +0.625 p = 0.05 |
Left Side vs. Plasma | Right Side vs. Plasma | Plasma vs. Plasma |
LPAsp vs. PlAla r = +0.751 p = 0.01 LPAsp vs. PlAsp r = +0.660 p = 0.03 LPAsp vs. PlCys r = +0.700 p = 0.0 LPAsp vs. PlGlu r = +0.618 p = 0.05 LAAla vs. PlAsp r = +0.670 p = 0.03 LAAla vs. PlCys r = +0.670 p = 0.03 LAAla vs. PlGlu r = +0.672 p = 0.03 | RPAla vs. PlAla r = +0.617 p = 0.05 RPCys vs. PlAla r = +0.849 p = 0.001 RPCys vs. PlAsp r = +0.898 p = 0.0004 RPCys vs. PlCys r = +0.741 p = 0.01 RPCys vs. PlGlu r = +0.907 p = 0.0003 RPGlu vs. PlAla r = +0.919 p = 0.0002 RPGlu vs. PlAsp r = +0.933 p = <0.0001 RPGlu vs. PlCys r = +0.859 p = 0.001 RPGlu vs. PlGlu r = +0.936 p = <0.0001 | PlAla vs. PlAsp r = +0.926 p = 0.0001 PlAla vs. PlCys r = +0.892 p = 0.0005 PlAla vs. PlGlu r = +0.927 p = 0.0001 PlAsp vs. PlCys r = +0.905 p = 0.0003 PlAsp vs. PlGlu r = +0.995 p = <0.0001 PlCys vs. PlGlu r = +0.906 p = 0.0003 |
Left Side vs. SBP | Right Side vs. SBP | |
LAAsp vs. SBP r = −0.685 p = 0.02 LACys vs. SBP r = −0.806 p = 0.004 LAGlu vs. SBP r = −0.650 p = 0.04 | RAGlu vs. SBP r = +0.754 p = 0.01 |
Lesioned Right SHR | ||
Intra-Hemispheric | Inter-Hemispheric | |
Lesioned Right (Left vs. Left) | Lesioned Right (Right vs. Right) | Lesioned Right (Left vs. Right) |
LPAla vs. LPAsp r = +0.769 p = 0.009 LPAla vs. LPCys r = +0.624 p = 0.05 LPAsp vs. LPCys r = +0.773 p = 0.008 LAAla vs. LAAsp r = +0.759 p = 0.01 LHAla vs. LHAsp r = +0.970 p = <0.0001 LHAla vs. LHCys r = +0.977 p = <0.0001 LHAla vs. LHGlu r = +0.989 p = <0.0001 LHAsp vs. LHCys r = +0.997 p = <0.0001 LHAsp vs. LHGlu r = +0.992 p = <0.0001 LHCys vs. LHGlu r = +0.993 p = <0.0001 LAGlu vs. LHAla r = +0.619 p = 0.05 LAGlu vs. LHAsp r = +0.646 p = 0.04 LAGlu vs. LHCys r = +0.630 p = 0.05 | RHAsp vs. RHGlu r = +0.744 p = 0.01 RHCys vs. RHGlu r = +0.679 p = 0.03 RPAla vs. RAAsp r = +0.724 p = 0.01 RPCys vs. RACys r = −0.683 p = 0.02 RPGlu vs. RHAsp r = −0.790 p = 0.006 RAAsp vs. RHCys r = +0.698 p = 0.02 RAGlu vs. RHAsp r = +0.653 p = 0.02 | LPCys vs. RPGlu r = +0.627 p = 0.05 LPGlu vs. RAGlu r = +0.635 p = 0.04 LHAla vs. RAGlu r = −0.624 p = 0.05 LHAsp vs. RAAla r = −0.636 p = 0.04 LHGlu vs. RAAla r = −0.626 p = 0.05 LHGlu vs. RAGlu r = −0.615 p = 0.05 |
Left Side vs. Plasma | Right Side vs. Plasma | Plasma vs. Plasma |
LPGlu vs. PlGlu r = +0.677 p = 0.03 | RHAsp vs. PlAla r = −0.638 p = 0.04 RHAsp vs. PlCys r = −0.677 p = 0.03 RHCys vs. PlCys r = −0.663 p = 0.03 | PlAla vs. PlCys r = +0.938 p = <0.0001 PlAla vs. PlGlu r = +0.734 p = 0.01 PlAsp vs. PlCys r = +0.744 p = 0.01 PlAsp vs. PlGlu r = +0.743 p = 0.01 PlCys vs. PlGlu r = +0.780 p = 0.007 |
Left Side vs. SBP | Right Side vs. SBP | |
No correlations | No correlations |
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Banegas, I.; Prieto, I.; Segarra, A.B.; Vives, F.; Martínez-Cañamero, M.; Durán, R.; Luna, J.d.D.; Domínguez-Vías, G.; Ramírez-Sánchez, M. Aminopeptidase Activities Interact Asymmetrically between Brain, Plasma and Systolic Blood Pressure in Hypertensive Rats Unilaterally Depleted of Dopamine. Biomedicines 2022, 10, 2457. https://doi.org/10.3390/biomedicines10102457
Banegas I, Prieto I, Segarra AB, Vives F, Martínez-Cañamero M, Durán R, Luna JdD, Domínguez-Vías G, Ramírez-Sánchez M. Aminopeptidase Activities Interact Asymmetrically between Brain, Plasma and Systolic Blood Pressure in Hypertensive Rats Unilaterally Depleted of Dopamine. Biomedicines. 2022; 10(10):2457. https://doi.org/10.3390/biomedicines10102457
Chicago/Turabian StyleBanegas, Inmaculada, Isabel Prieto, Ana Belén Segarra, Francisco Vives, Magdalena Martínez-Cañamero, Raquel Durán, Juan de Dios Luna, Germán Domínguez-Vías, and Manuel Ramírez-Sánchez. 2022. "Aminopeptidase Activities Interact Asymmetrically between Brain, Plasma and Systolic Blood Pressure in Hypertensive Rats Unilaterally Depleted of Dopamine" Biomedicines 10, no. 10: 2457. https://doi.org/10.3390/biomedicines10102457