Umbelliferone-Based Fluorescent Probe for Selective Recognition of Hydrogen Sulfide and Its Bioimaging in Living Cells and Zebrafish
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials and Instruments
2.2. Synthesis of Probe 1
2.3. General Procedure for Absorption and Fluorescence Measurements
2.4. Cell Imaging
2.5. Zebrafish Imaging
3. Results and Discussion
3.1. Synthesis and Characterization
3.2. Optical Behavior of 1 to H2S
3.3. Time and pH-Dependent Fluorescence Response of 1 to H2S
3.4. Selectivity and Detection Limit of 1 to H2S
3.5. Cytotoxicity of 1
3.6. Imaging of H2S in Cells by 1
3.7. Imaging of H2S in Zebrafish by 1
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fang, Y.; Luo, F.; Cao, Z.; Peng, C.; Dehaen, W. Umbelliferone-Based Fluorescent Probe for Selective Recognition of Hydrogen Sulfide and Its Bioimaging in Living Cells and Zebrafish. Chemosensors 2022, 10, 427. https://doi.org/10.3390/chemosensors10100427
Fang Y, Luo F, Cao Z, Peng C, Dehaen W. Umbelliferone-Based Fluorescent Probe for Selective Recognition of Hydrogen Sulfide and Its Bioimaging in Living Cells and Zebrafish. Chemosensors. 2022; 10(10):427. https://doi.org/10.3390/chemosensors10100427
Chicago/Turabian StyleFang, Yuyu, Fan Luo, Zhixing Cao, Cheng Peng, and Wim Dehaen. 2022. "Umbelliferone-Based Fluorescent Probe for Selective Recognition of Hydrogen Sulfide and Its Bioimaging in Living Cells and Zebrafish" Chemosensors 10, no. 10: 427. https://doi.org/10.3390/chemosensors10100427