Effect of Zinc Excess in Substrate on Physiological Responses of Sinapis alba L.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Growth Conditions, and Treatments
2.2. Determination of Zinc Content
2.3. Analysis of Growth-Related Parameters
2.4. Photosynthetic Pigment Content Measurement
2.5. Gas Exchange and Chlorophyll a Fluorescence Assay
2.6. The Free Proline Content
2.7. Determination of Lipid Peroxidation
2.8. The Relative Electrolyte Leakage (REL) Assay
2.9. Statistical Analysis
3. Results
3.1. Effect of Zn Excess on Zn Content in S. alba Seedlings
3.2. Effect of Zn Excess on Shoot Growth and Biomass Accumulation of S. alba Seedlings
3.3. Effect of Zn Excess on Photosynthetic Apparatus (PSA) of S. alba Seedlings
3.4. Effect of Zn Excess on Water Content of S. alba Seedlings
3.5. Effect of Zn Excess on Relative Electrolyte Leakage (REL), Malondialdehyde (MDA), and Proline Content in S. alba Seedlings
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zn Concentration, mg kg−1 Substrate | Zn Content, ppm | |
---|---|---|
Shoot | Root | |
5 | 187.32 ± 20.60 d | 115.01 ± 14.80 e |
50 | 2505.31 ± 206.02 c | 1504.60 ± 125.96 c |
100 | 2620.35 ± 215.22 b | 2708.28 ± 222.26 b |
150 | 3118.52 ± 311.08 a | 3340.07 ± 272.80 a |
Zn Concentration, mg kg−1 Substrate | Shoot Height, cm | Shoot FW, g | Shoot DW, g |
---|---|---|---|
5 | 26.22 ± 1.84 a | 1.27 ± 0.14 a | 0.11 ± 0.01 a |
50 | 23.49 ± 1.26 a | 1.36 ± 0.15 a | 0.11 ± 0.01 a |
100 | 11.66 ± 0.51 b | 0.46 ± 0.04 b | 0.05 ± 0.01 b |
150 | 6.94 ± 0.48 c | 0.27 ± 0.03 c | 0.04 ± 0.01 c |
Zn Concentration, mg kg−1 Substrate | Chlorophyll a, mg g−1 FW | Chlorophyll b, mg g−1 FW | Total Chlorophyll (a+b), mg g−1 FW | Carotenoids, mg g−1 FW | Chlorophyll Content in LHCII, % |
---|---|---|---|---|---|
5 | 0.98 ± 0.03 a | 0.38 ± 0.02 a | 1.36 ± 0.04 a | 0.28 ± 0.01 a | 0.18 ± 0.02 a |
50 | 0.94 ± 0.03 a | 0.41 ± 0.01 a | 1.35 ± 0.04 a | 0.25 ± 0.01 a | 0.20 ± 0.01 a |
100 | 0.62 ± 0.02 b | 0.26 ± 0.01 b | 0.88 ± 0.03 b | 0.18 ± 0.01 b | 0.09 ± 0.01 b |
150 | 0.38 ± 0.02 c | 0.16 ± 0.01 c | 0.53 ± 0.03 b | 0.13 ± 0.01 c | 0.03 ± 0.01 b |
Zn Concentration, mg kg−1 Substrate | WUE, µmol CO2 mmol H2O−1 | RWC, % |
---|---|---|
5 | 6.32 ± 0.44 a | 91.37 ± 0.17 a |
50 | 6.39 ± 0.61 a | 91.73 ± 0.18 a |
100 | 5.84 ± 0.19 a | 89.44 ± 0.31 b |
150 | 3.34 ± 0.24 b | 86.44 ± 0.45 c |
Zn Concentration, mg kg−1 Substrate | REL, % | MDA, nmol g−1 FW | Proline, mmol g−1 FW |
---|---|---|---|
5 | 10.80 ± 0.83 a | 1.98 ± 0.09 b | 6.11 ± 0.30 b |
50 | 11.30 ± 0.77 a | 2.10 ± 0.08 b | 16.40 ± 1.97 a |
100 | 11.00 ± 1.23 a | 2.77 ± 0.15 a | 22.95 ± 5.59 a |
150 | 12.30 ± 0.06 a | 2.67 ± 0.19 a | 17.53 ± 3.93 a |
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Repkina, N.; Nilova, I.; Kaznina, N. Effect of Zinc Excess in Substrate on Physiological Responses of Sinapis alba L. Plants 2023, 12, 211. https://doi.org/10.3390/plants12010211
Repkina N, Nilova I, Kaznina N. Effect of Zinc Excess in Substrate on Physiological Responses of Sinapis alba L. Plants. 2023; 12(1):211. https://doi.org/10.3390/plants12010211
Chicago/Turabian StyleRepkina, Natalia, Irina Nilova, and Natalia Kaznina. 2023. "Effect of Zinc Excess in Substrate on Physiological Responses of Sinapis alba L." Plants 12, no. 1: 211. https://doi.org/10.3390/plants12010211