Molecular Identification of African Nymphaea Species (Water Lily) Based on ITS, trnT-trnF and rpl16
, Justus Mulinge Munywoki 3,†, Qing-Feng Wang 3,4, Itambo Malombe 5, Zhi-Zhong Li 1,* and Jin-Ming Chen 1,*
Round 1
Reviewer 1 Report
The authors apply the combination of ITS and trnT-trnF (or rpl16) as the DNA barcoding of Nymphaea, which will enrich the DNA barcoding database of Nymphaea and provide a reference basis for studying taxonomy, phylogenetics and evolutionary origin of Nymphaea. But the English writing should be improved. Additionally, I have only few minor comments:
Line 38 change 'ability to purify water bodies' to 'water purification capacity'
Line 39 change taxa to lineage
Line 45 change 'It is a pity because' to 'Though'
Author Response
The authors apply the combination of ITS and trnT-trnF (or rpl16) as the DNA barcoding of Nymphaea, which will enrich the DNA barcoding database of Nymphaea and provide a reference basis for studying taxonomy, phylogenetics and evolutionary origin of Nymphaea. But the English writing should be improved. Additionally, I have only few minor comments.
Response:
Thanks for your comments. We have corrected our MS according to your suggestions. Please see below.
1. Line 38 change 'ability to purify water bodies' to 'water purification capacity'
Response:
We agree with your suggestion. We have modified “ability to purify water bodies” to “water purification capacity”.
Line 39 change taxa to lineage
Response:
We agree with your suggestion. We have modified “taxa” to “lineage”.
Line 45 change 'It is a pity because' to 'Though'
Response:
We agree with your suggestion. We have modified “It is a pity because” to “Though”.
Author Response File: 
Author Response.docx
Reviewer 2 Report
The work is of a high standard and of great interest. The research aims to identify suitable tools to clarify the taxonomy among the species belonging to the genus Nynphaea. The results obtained lay the groundwork for greater clarity in the classification and history of evolution of these species.
The methodology used is clear.
The discussion is well structured and the comparison with previous works clarifies the results obtained
Author Response
The work is of a high standard and of great interest. The research aims to identify suitable tools to clarify the taxonomy among the species belonging to the genus Nynphaea. The results obtained lay the groundwork for greater clarity in the classification and history of evolution of these species.
The methodology used is clear.
The discussion is well structured and the comparison with previous works clarifies the results obtained.
Response:
Thanks for your comments.
Author Response File: Author Response.docx
