Characterization and Functional Analysis of GhNAC82, A NAM Domain Gene, Coordinates the Leaf Senescence in Upland Cotton (Gossypium hirsutum L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials, Growth Conditions and Stress Treatments
2.2. Gene Cloning and Sequence Analysis
2.3. DNA, RNA Extraction, cDNA Synthesis, and Quantitative RT-PCR (qRT-PCR)
2.4. Vector Construction and Genetic Transformation of A. thaliana
2.5. Viruses Induced GhNAC82 Gene Silencing in Cotton
2.6. Determination of the Related Physiological Parameters
3. Results
3.1. Identification and Evolution Analysis of the GhNAC82
3.2. Predicted of Cis-Elements in Promoter Region of GhNAC82
3.3. Expression Patterns Analysis of GhNAC82 in Various Tissues
3.4. Phenotypic Changes and Expression Patterns of GhNAC82 during Cotyledon Senescence
3.5. Expression of the GhNAC82 in Leaves Was Significantly Induced by Abiotic Stresses
3.6. The Expression Patterns of GhNAC82 under Different Hormones
3.7. Overexpression of GhNAC82 in Transgenic Arabidopsis Promotes Leaf Senescence
3.8. Virus-Induced Gene Silencing of GhNAC82 Exhibited Stronger Vegetative Growth
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, C.; Li, T.; Liu, Q.; Li, L.; Feng, Z.; Yu, S. Characterization and Functional Analysis of GhNAC82, A NAM Domain Gene, Coordinates the Leaf Senescence in Upland Cotton (Gossypium hirsutum L.). Plants 2022, 11, 1491. https://doi.org/10.3390/plants11111491
Wang C, Li T, Liu Q, Li L, Feng Z, Yu S. Characterization and Functional Analysis of GhNAC82, A NAM Domain Gene, Coordinates the Leaf Senescence in Upland Cotton (Gossypium hirsutum L.). Plants. 2022; 11(11):1491. https://doi.org/10.3390/plants11111491
Chicago/Turabian StyleWang, Chenlei, Tengyu Li, Qibao Liu, Libei Li, Zhen Feng, and Shuxun Yu. 2022. "Characterization and Functional Analysis of GhNAC82, A NAM Domain Gene, Coordinates the Leaf Senescence in Upland Cotton (Gossypium hirsutum L.)" Plants 11, no. 11: 1491. https://doi.org/10.3390/plants11111491