Transcriptomic and Metabolic Profiling of Kenaf Stems under Salinity Stress
Abstract
:1. Introduction
2. Results
2.1. Illumina Sequencing and Assembly
2.2. Gene Annotation and Function Classification
2.3. Differential Expression Analyses
2.4. Metabolic Profiling of Kenaf Stems
2.5. Lignin and Phytohormone Contents, and Underlying Gene Expressions
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. RNA Extraction, Library Preparation, and Sequencing
4.3. Data Assembly and Annotation
4.4. Quantification of Gene Expression Levels and Differential Expression Analysis
4.5. Quantitative Reverse Transcription PCR (qRT-PCR)
4.6. Measurements of Lignin Monomers
4.7. Generation of Stem Slices
4.8. Metabolic Profiling of Kenaf Stems
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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An, X.; Chen, J.; Liu, T.; Li, W.; Luo, X.; Zou, L. Transcriptomic and Metabolic Profiling of Kenaf Stems under Salinity Stress. Plants 2022, 11, 1448. https://doi.org/10.3390/plants11111448
An X, Chen J, Liu T, Li W, Luo X, Zou L. Transcriptomic and Metabolic Profiling of Kenaf Stems under Salinity Stress. Plants. 2022; 11(11):1448. https://doi.org/10.3390/plants11111448
Chicago/Turabian StyleAn, Xia, Jie Chen, Tingting Liu, Wenlue Li, Xiahong Luo, and Lina Zou. 2022. "Transcriptomic and Metabolic Profiling of Kenaf Stems under Salinity Stress" Plants 11, no. 11: 1448. https://doi.org/10.3390/plants11111448