Genome-Wide Identification and Functional Differentiation of Fatty Acid Desaturase Genes in Olea europaea L.
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification of FAD Genes in Olive
2.2. Phylogenetic Analysis of FAD Genes in Different Species
2.3. Gene Characters and Protein Subcellular Localization
2.4. The Roles of FAD Genes in Olive Morphology
2.5. Stress Response of FAD Genes in Olive
3. Discussion
3.1. FAD Genes in Different Plants
3.2. Functional Differentiation of FAD Genes in Olive
4. Materials and Methods
4.1. Database Search and FAD Genes Retrieval
4.2. Phylogenetic Analysis
4.3. Physicochemical Character and Subcellular Localization Prediction
4.4. Expression Analysis of FAD Genes in Olive
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cultivated/Wild Olive | Groups | Gene Name | Gene ID | Chr./Scaffold | Protein Length/a.a. | Molecular Weight/Da | Isoelectric Point |
---|---|---|---|---|---|---|---|
O. europaea cv. Farga | FAB2/SAD | OeFAB2.1 | OE6A002165 | s09619 | 386 | 43,985.23 | 6.2 |
OeFAB2.2 | OE6A012975 | s05995 | 396 | 45,549.82 | 6.08 | ||
OeFAB2.3 | OE6A020845 | s05960 | 366 | 41,893.6 | 5.44 | ||
OeFAB2.4 | OE6A024078 | s03906 | 94 | 10,932.6 | 6.83 | ||
OeFAB2.5 | OE6A033129 | s03906 | 159 | 18,196.6 | 5.96 | ||
OeFAB2.6 | OE6A048475 | s02255 | 390 | 44,535.91 | 6.11 | ||
OeFAB2.7 | OE6A060676 | s03906 | 208 | 23,921.35 | 8.45 | ||
OeFAB2.8 | OE6A089828 | s09854 | 399 | 45,584.11 | 6.58 | ||
OeFAB2.9 | OE6A108617 | s03845 | 291 | 33,386.07 | 5.38 | ||
OeFAB2.10 | OE6A118450 | s09608 | 283 | 32,301.89 | 5.68 | ||
ADS | OeADS.1 | OE6A005264 | s08189 | 384 | 43,921.63 | 9.36 | |
OeADS.2 | OE6A080903 | s00065 | 375 | 43,343.08 | 9.28 | ||
OeADS.3 | OE6A090227 | s00065 | 384 | 43,987.69 | 9.36 | ||
OeADS.4 | OE6A120700 | s08189 | 151 | 17,260.67 | 6.64 | ||
DES | OeDES | OE6A026807 | s03867 | 332 | 38,815.97 | 7.88 | |
FAD4 | OeFAD4.1 | OE6A064387 | s09642 | 305 | 34,001.84 | 8.19 | |
OeFAD4.2 | OE6A070143 | s09790 | 305 | 33,954.76 | 8.19 | ||
OeFAD4.3 | OE6A082617 | s07944 | 237 | 26,292.52 | 8.85 | ||
SLD | OeSLD.1 | OE6A013963 | s00013 | 447 | 51,607.52 | 8.68 | |
OeSLD.2 | OE6A023328 | s07941 | 447 | 51,536.29 | 7.93 | ||
OeSLD.3 | OE6A037200 | s07705 | 447 | 51,568.46 | 8.68 | ||
OeSLD.4 | OE6A037564 | s00022 | 448 | 51,531.57 | 8.76 | ||
OeSLD.5 | OE6A087532 | s09955 | 447 | 51,649.44 | 7.63 | ||
OeSLD.6 | OE6A107382 | s05784 | 447 | 51,823.75 | 8.7 | ||
OeSLD.7 | OE6A114237 | s01961 | 371 | 43,394.75 | 8.6 | ||
ω-3 | OeFAD3.1 | OE6A024624 | s02233 | 395 | 45,696.39 | 7.17 | |
OeFAD3.2 | OE6A086562 | s09646 | 164 | 18,813.28 | 7.77 | ||
OeFAD3.3 | OE6A109248 | s06024 | 158 | 17,919.2 | 6.19 | ||
OeFAD3.4 | OE6A113752 | s00046 | 172 | 19,727.44 | 6.7 | ||
OeFAD7 | OE6A117679 | s04425 | 64 | 7269.06 | 6.08 | ||
OeFAD8.1 | OE6A074266 | s07757 | 461 | 52,736.2 | 7.79 | ||
OeFAD8.2 | OE6A075849 | s03965 | 436 | 49,806.14 | 9.18 | ||
ω-6 | OeFAD2.1 | OE6A011870 | s00121 | 383 | 43,916.5 | 8.73 | |
OeFAD2.2 | OE6A019200 | s05069 | 381 | 44,216.96 | 8.48 | ||
OeFAD2.3 | OE6A051290 | s00121 | 383 | 43,907.49 | 8.73 | ||
OeFAD2.4 | OE6A067271 | s07908 | 381 | 44,167.93 | 8.62 | ||
OeFAD2.5 | OE6A069627 | s01964 | 381 | 44,182.9 | 8.46 | ||
OeFAD2.6 | OE6A085290 | s05804 | 380 | 43,729.63 | 8.62 | ||
OeFAD2.7 | OE6A098403 | s07710 | 383 | 44,064.09 | 9.01 | ||
OeFAD6 | OE6A116067 | s07955 | 443 | 51,281.56 | 9.21 | ||
O. europaea var. Sylvestris | FAB2/SAD | OeuFAB2.1 | Oeu009943 | scaffold13793 | 366 | 41,906.65 | 5.53 |
OeuFAB2.2 | Oeu015714 | scaffold1625 | 208 | 23,865.24 | 7.02 | ||
OeuFAB2.3 | Oeu024466 | scaffold2105 | 187 | 21,435.4 | 6.53 | ||
OeuFAB2.4 | Oeu025716 | scaffold2199 | 386 | 43,746.94 | 6.03 | ||
OeuFAB2.5 | Oeu040504 | scaffold369 | 390 | 44,622.01 | 6.11 | ||
OeuFAB2.6 | Oeu048444 | scaffold5181 | 106 | 12,242.2 | 8.88 | ||
OeuFAB2.7 | Oeu050331 | scaffold564 | 407 | 46,693.26 | 6.03 | ||
ADS | OeuADS | Oeu027810 | scaffold2325 | 314 | 36,183.62 | 8.71 | |
DES | OeuDES.1 | Oeu027812 | scaffold2325 | 110 | 12,813.52 | 6.96 | |
OeuDES.2 | Oeu053967 | scaffold655 | 331 | 38,746.7 | 7.31 | ||
FAD4 | OeuFAD4.1 | Oeu042929 | scaffold41 | 305 | 34,047.89 | 8.24 | |
OeuFAD4.2 | Oeu062594 | chr7 | 237 | 26,199.36 | 8.62 | ||
SLD | OeuSLD.1 | Oeu003987 | scaffold1142 | 447 | 51,536.29 | 7.93 | |
OeuSLD.2 | Oeu009350 | scaffold1349 | 447 | 51,753.64 | 8.33 | ||
OeuSLD.3 | Oeu036586 | scaffold314 | 450 | 51,825.81 | 8.65 | ||
OeuSLD.4 | Oeu036587 | scaffold314 | 447 | 51,588.45 | 8.68 | ||
OeuSLD.5 | Oeu063672 | scaffold949 | 448 | 51,531.57 | 8.76 | ||
ω-3 | OeuFAD3.1 | Oeu004670 | scaffold1172 | 373 | 43,368.95 | 8.35 | |
OeuFAD3.2 | Oeu015599 | scaffold162 | 373 | 43,253.83 | 8.61 | ||
OeuFAD8.1 | Oeu004694 | scaffold1173 | 436 | 49,840.16 | 9.18 | ||
OeuFAD8.2 | Oeu033588 | scaffold281 | 473 | 54,190.85 | 8.14 | ||
OeuFAD8.3 | Oeu050958 | scaffold58 | 63 | 7167.97 | 6.47 | ||
ω-6 | OeuFAD2.1 | Oeu007766 | scaffold1284 | 383 | 44,071.13 | 9.09 | |
OeuFAD2.2 | Oeu013924 | scaffold1547 | 381 | 44,182.9 | 8.46 | ||
OeuFAD2.3 | Oeu033739 | scaffold283 | 383 | 43,948.5 | 8.73 | ||
OeuFAD2.4 | Oeu058547 | scaffold782 | 381 | 44,185.95 | 8.62 | ||
OeuFAD2.5 | Oeu061755 | scaffold885 | 380 | 43,685.55 | 8.45 |
Groups | A. thaliana | O. sativa | G. max | B. rapa | B. oleracea | J. regia | O. europaea cv. Farga | O. europaea var. Sylvestris |
---|---|---|---|---|---|---|---|---|
FAB2/SAD | 7 | 8 | 5 | 7 | 6 | 9 | 10 | 7 |
Total soluble FAD | 7 | 8 | 5 | 7 | 6 | 9 | 10 | 7 |
ADS | 9 | 0 | 2 | 20 | 18 | 1 | 4 | 1 |
DES | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 2 |
FAD4 | 1 | 1 | 0 | 4 | 4 | 1 | 3 | 2 |
SLD | 2 | 1 | 6 | 4 | 5 | 3 | 7 | 5 |
ω-3 | 3 | 4 | 9 | 6 | 7 | 8 | 7 | 5 |
ω-6 | 2 | 4 | 17 | 3 | 3 | 6 | 8 | 5 |
Total membrane-bound FAD | 18 | 11 | 36 | 38 | 38 | 21 | 30 | 20 |
Total FAD | 25 | 19 | 41 | 45 | 44 | 30 | 40 | 27 |
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Niu, E.; Gao, S.; Hu, W.; Zhang, C.; Liu, D.; Shen, G.; Zhu, S. Genome-Wide Identification and Functional Differentiation of Fatty Acid Desaturase Genes in Olea europaea L. Plants 2022, 11, 1415. https://doi.org/10.3390/plants11111415
Niu E, Gao S, Hu W, Zhang C, Liu D, Shen G, Zhu S. Genome-Wide Identification and Functional Differentiation of Fatty Acid Desaturase Genes in Olea europaea L. Plants. 2022; 11(11):1415. https://doi.org/10.3390/plants11111415
Chicago/Turabian StyleNiu, Erli, Song Gao, Wenjun Hu, Chengcheng Zhang, Daqun Liu, Guoxin Shen, and Shenlong Zhu. 2022. "Genome-Wide Identification and Functional Differentiation of Fatty Acid Desaturase Genes in Olea europaea L." Plants 11, no. 11: 1415. https://doi.org/10.3390/plants11111415