Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis
Abstract
:1. Introduction
2. Results
2.1. Wood Essential Oil of Calocedrus formosana (CFEO) Inhibits Mushroom Tyrosinase Actvity
2.2. CFEO Inhibits α-MSH-FSK-Induced Melanin Biosynthesis in B16-F10 Cells
2.3. CFEO Inhibits Melanogeneis Regulatory Proteins in B16-F10 Cells
2.4. Chemical Composition of CFEO
2.5. Effects of CFEO Major Constituents on Melanin Production
3. Discussion
4. Materials and Methods
4.1. Preparation of C. formosana Wood Essential Oil (CFEO)
4.2. Chemicals and Reagents
4.3. Cell Culture and Cell Viability Assay
4.4. Mushroom Tyrosinase Assay
4.5. Determination of Melanin Content
4.6. Protein Extraction and Western Blot Analysis
4.7. GC–MS Analysis
4.8. Unknown Compounds Identification
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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RT(min) | Compounds | Area (%) | KI | Identification |
---|---|---|---|---|
11.5 | Camphene | 1.11 | 951 | MS KI ST |
14.21 | 3-Carene | 0.29 | 1009 | MS KI ST |
14.51 | 1,4-Cineol | 0.82 | 1016 | MS KI ST |
14.94 | p-Cymene | 2.24 | 1026 | MS KI ST |
15.18 | limonene | 0.20 | 1031 | MS KI ST |
15.36 | Eucalyptol (1,8-Cineol) | 0.43 | 1035 | MS KI ST |
16.54 | trans-Sabinenehydrate | 0.17 | 1060 | MS KI |
17.61 | 1-Methylindan | 0.54 | 1082 | MS KI |
17.83 | α-Terpinolen | 0.17 | 1086 | MS KI ST |
18.03 | p-Cymenene | 0.47 | 1090 | MS KI ST |
20.73 | Camphor | 1.89 | 1148 | MS KI ST |
22.34 | Terpinen-4-ol | 12.23 | 1181 | MS KI ST |
22.45 | Myrtenol | 1.18 | 1183 | MS KI ST |
23.02 | α-Terpineol | 23.47 | 1194 | MS KI ST |
23.55 | Berbenone | 2.81 | 1205 | MS KI ST |
25.71 | Piperitone | 3.44 | 1254 | MS KI |
26.79 | trans-2-Caren-4-ol | 1.36 | 1277 | MS KI ST |
27.75 | Thymol | 5.30 | 1296 | MS KI ST |
28.4 | Carvacrol | 0.47 | 1311 | MS KI ST |
29.29 | Shonanic acid (1) | 10.45 | 1332 | MS ST |
30.68 | Perilla aldehyde | 1.44 | 1364 | MS KI ST |
31.31 | Thujic acid (2) | 1.65 | 1378 | MS ST |
32.00 | Chaminic acid (3) | 0.13 | 1393 | MS ST |
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Hsiao, W.-W.; Kumar, K.J.S.; Lee, H.-J.; Tsao, N.-W.; Wang, S.-Y. Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis. Plants 2022, 11, 62. https://doi.org/10.3390/plants11010062
Hsiao W-W, Kumar KJS, Lee H-J, Tsao N-W, Wang S-Y. Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis. Plants. 2022; 11(1):62. https://doi.org/10.3390/plants11010062
Chicago/Turabian StyleHsiao, Wen-Wei, K. J. Senthil Kumar, Hui-Ju Lee, Nai-Wen Tsao, and Sheng-Yang Wang. 2022. "Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis" Plants 11, no. 1: 62. https://doi.org/10.3390/plants11010062