Genetic and Morpho-Agronomic Characterization of Sicilian Tetraploid Wheat Germplasm
Abstract
:1. Introduction
2. Results
2.1. Genetic Profile
2.2. Genetic Structure
2.3. Phylogenetic Analysis
2.4. Morpho-Agronomic Traits
3. Discussion
4. Materials and Methods
4.1. Plant Material and DNA Extraction
4.2. Genotyping by Using SSR Markers
4.3. Statistical Analysis
4.4. Genetic Distance
4.5. Morpho-Agronomic Characterization
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
Statement
References
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Accession | Abbreviation | Origin (Farm) |
---|---|---|
Rivet wheat landraces (Bufala and Bufala-related group) | ||
Bufala Bianca 02 | BB-02 | Randazzo (Catania)—C.da Flascio 1 |
Bufala Bianca 03 | BB-03 | Gangi (Palermo)—C.da Cavaliere 1 |
Bufala Bianca 04 | BB-04 | USDA 157984 2 |
Bufale Cerami 01 | BC-01 | Cerami (Enna) 1 |
Bufale Flascio 01 | BF-01 | Randazzo (Catania)—C.da Flascio 1 |
Bufale Gangi 03 | BG-03 | Gangi (Palermo) 3 |
Bufala Nera Corta 01 | BNC-01 | Maletto (Catania)—C.da Piana 1 |
Bufala Nera Corta 02 | BNC-02 | IPK 3517 2 |
Bufala Nera Lunga 01 | BNL-01 | Randazzo (Catania)—C.da Flascio 1 |
Bufala Nera Lunga 02 | BNL-02 | IPK 4291 2 |
Bufala Nera Lunga 03 | BNL-03 | Randazzo (Catania)—C.da Flascio 1 |
Bufala Nera Lunga 04 | BNL-04 | Gangi (Palermo)—C.da Mengarda 1 |
Bufala Nera Lunga 05 | BNL-05 | Maletto (Catania)—C.da Cimitero 1 |
Bufala Rossa Corta a 01 | BRCa-01 | Randazzo (Catania)—C.da Flascio 1 |
Bufala Rossa Corta b 01 | BRCb-01 | Randazzo (Catania)—C.da Flascio 1 |
Bufala Rossa Lunga 01 | BRL-01 | Randazzo (Catania)—C.da Flascio 1 |
Bufala Rossa Lunga 03 | BRL-03 | Maletto (Catania)—C.da Piana 1 |
Bufale Salice 01 | BS-01 | Salice village (Messina) 4 |
Bufale Salice 02 | BS-02 | Salice village (Messina) 4 |
Bufale Troina 01 | BT-01 | Troina (Enna) 1 |
Bivona 03 | BIV-03 | Gangi (Palermo)—C.da Mengarda 1 |
Bivona 04 | BIV-04 | Santo Stefano Quisquina (Palermo) 1 |
Ciciredda 01 | CIC-01 | Maletto (Catania)—C.da Piana 1 |
Ciciredda 02 | CIC-02 | Randazzo (Catania)—C.da Flascio1 |
Ciciredda 03 | CIC-03 | IPK TRI 28458 2 |
Paola 01 | PAO-01 | Maletto (Catania)—C.da S. Venera 1 |
Paola 02 | PAO-02 | Randazzo (Catania)—C.da Flascio 1 |
Durum wheat improved varieties (Outgroup) | ||
Bidì 03 | BIDI-03 | IPK TRI 26213 5 |
Capeiti | CAP-8 | Patended as Capeiti 8 (Eiti 6 × Cappelli) in 1969 at the Stazione Sperimentale di Granicoltura |
Simeto | SIM | Variety (Capeiti 8 × Valnova) patended in 1988 at Stazione Sperimentale di Granicoltura |
Genotype Group | N° of Samples | Na | Ho | He | PIC |
---|---|---|---|---|---|
Bufala | 20 | 5.56 | 0.35 | 0.55 | 0.50 |
Bufala-related | 7 | 4.16 | 0.34 | 0.55 | 0.48 |
Outgroup | 3 | 2.25 | 0.27 | 0.42 | 0.32 |
Total | 30 | 6.98 | 0.34 | 0.60 | 0.56 |
Genotype Group | DAPC | ||
---|---|---|---|
C1 | C2 | C3 | |
Bufala | 5 | 13 | 2 |
Bufala-related | 3 | 4 | 0 |
Outgroup | 0 | 0 | 3 |
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Sicilia, A.; Anastasi, U.; Bizzini, M.; Montemagno, S.; Nicotra, C.; Blangiforti, S.; Spina, A.; Cosentino, S.L.; Lo Piero, A.R. Genetic and Morpho-Agronomic Characterization of Sicilian Tetraploid Wheat Germplasm. Plants 2022, 11, 130. https://doi.org/10.3390/plants11010130
Sicilia A, Anastasi U, Bizzini M, Montemagno S, Nicotra C, Blangiforti S, Spina A, Cosentino SL, Lo Piero AR. Genetic and Morpho-Agronomic Characterization of Sicilian Tetraploid Wheat Germplasm. Plants. 2022; 11(1):130. https://doi.org/10.3390/plants11010130
Chicago/Turabian StyleSicilia, Angelo, Umberto Anastasi, Michele Bizzini, Stefania Montemagno, Carmelo Nicotra, Sebastiano Blangiforti, Alfio Spina, Salvatore Luciano Cosentino, and Angela Roberta Lo Piero. 2022. "Genetic and Morpho-Agronomic Characterization of Sicilian Tetraploid Wheat Germplasm" Plants 11, no. 1: 130. https://doi.org/10.3390/plants11010130