Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency
Abstract
:1. Introduction
2. Results
2.1. Survival Rate
2.2. Growth Change Rate
2.3. Trait/Morphological Mutation Rate
2.4. Genotype Analysis of Gerbera Mutants Obtained by Ion Beam Irradiation
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Procedure
4.3. Survival Rate
4.4. Growth Change Rate
4.5. Trait/Morphological Mutation Rate
4.6. Genotype Analysis of Gerbera Mutants Obtained by Ion Beam Irradiation
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Classification of Mutant Phenotypes | Irradiation treatment | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
C | Ar | Fe | Total | |||||||||||||
2 | 5 | 10 | 25 | 1 | 2 | 5 | 10 | 25 | 1 | 2 | 5 | 10 | 25 | |||
Single mutation | Flower color | 0 | 1 | 2 | 0 | 1 | 4 | 5 | 1 | 1 | 2 | 2 | 2 | 0 | 0 | 21 |
Petal slender | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | |
Petal sword | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |
Receptive shape | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | |
Semidouble | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |
Male sterile | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | |
Complex mutation | Flower color/Petal slender | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Flower color/Receptive shape | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |
Flower color/Semidouble | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | |
Flower color/Male sterile | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |
Petal slender/Male sterile | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | |
Chimeric mutation | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | |
Total | 0 | 3 | 4 | 0 | 1 | 6 | 11 | 4 | 1 | 3 | 4 | 7 | 0 | 0 | 44 |
Trait | Line Name | No. of Read | No. of Seqenced Bases (Mbp) | %Q30 z | No. of Mutant-Specific Markers y | |
---|---|---|---|---|---|---|
Wild type | Normal | 0 Gy | 5,289,248 | 534 | 88.6 | - |
Mutant | Flower color | Fe 2 Gy- 87 | 5,355,904 | 541 | 89.5 | 1 |
Petal slender | Ar 5 Gy- 29 | 5,364,690 | 542 | 89.2 | 6 | |
Male sterile | Fe 5 Gy- 34 | 5,045,048 | 510 | 88.9 | 31 |
Elements | Ion Notation | Energy | LET |
---|---|---|---|
(MeV·µ−1) | (keV·µm−1) | ||
Carbon | 12C6+ | 290 | 14 |
Argon | 40Ar18+ | 500 | 89 |
Iron | 56Fe26+ | 500 | 185 |
Ion | Absorbed Dose (Gy) | Number of Irradiated Plants | Number of Surviving Plants |
---|---|---|---|
C | 0 | 201 | 201 |
2 | 187 | 184 | |
5 | 210 | 205 | |
10 | 185 | 174 | |
25 | 123 | 14 | |
Ar | 0 | 63 | 63 |
1 | 96 | 92 | |
2 | 101 | 98 | |
5 | 78 | 71 | |
10 | 65 | 23 | |
25 | 86 | 7 | |
Fe | 0 | 154 | 122 |
1 | 181 | 171 | |
2 | 160 | 146 | |
5 | 151 | 53 | |
10 | 166 | 19 | |
25 | 156 | 4 |
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Hosoguchi, T.; Uchiyama, Y.; Komazawa, H.; Yahata, M.; Shimokawa, T.; Tominaga, A. Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency. Plants 2021, 10, 1480. https://doi.org/10.3390/plants10071480
Hosoguchi T, Uchiyama Y, Komazawa H, Yahata M, Shimokawa T, Tominaga A. Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency. Plants. 2021; 10(7):1480. https://doi.org/10.3390/plants10071480
Chicago/Turabian StyleHosoguchi, Tomoya, Yuna Uchiyama, Hinata Komazawa, Masaki Yahata, Takashi Shimokawa, and Akiyoshi Tominaga. 2021. "Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency" Plants 10, no. 7: 1480. https://doi.org/10.3390/plants10071480