QTL Validation and Development of SNP-Based High Throughput Molecular Markers Targeting a Genomic Region Conferring Narrow Root Cone Angle in Aerobic Rice Production Systems
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Variation of the Segregating Populations
2.2. SNP Selection Using 3KRGP
2.3. Molecular Marker Development and Quality Metrics of KASP Markers Targeting qRCA QTL
2.4. QTL Validation and Effects across Genetic Backgrounds
2.5. Performance of Newly Developed KASP Markers Targeting qRCA4 Locus
2.6. Identification of Candidate Genes and Possible Donors qRCA4 in 3K in All Subpopulations
3. Discussion
3.1. qRCA4 Is Effective across Multiple Genetic Backgrounds
3.2. KASP-Based SNP Markers Tagging qRCA4 Were Developed
3.3. 3KRGP and Database Search Facilitated the Identification of Potential Donors and Candidate Genes
4. Materials and Methods
4.1. Plant Materials
4.2. Phenotyping for RCA
4.3. Development of KASP SNP Molecular Markers
4.4. Molecular Marker and QTL Validation
4.5. Identification of Candidate Genes and Possible Donors Present in 3KRGP
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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IRLA n = 196 | IRRL n = 331 | IRNO n = 283 | |
---|---|---|---|
Mean | 90 | 98 | 85 |
Min | 67 | 68 | 63 |
Max | 124 | 130 | 116 |
p value | <0.0001 | <0.0001 | <0.0001 |
Heritability | 0.69 | 0.67 | 0.68 |
Check Genotypes | |||
IRAT109 | 71 | 79 | 77 |
Langi | 111 | 106 | 102 |
RL11 | ND | 122 | 95 |
Norin PL8 | 93 | 98 | 94 |
Sherpa | 108 | 117 | 101 |
Reiziq | 121 | 124 | 117 |
SNP ID | Chr | Position | QTL | SNP | Favourable Allele | Call Rate | FPR (%) | FNR (%) |
---|---|---|---|---|---|---|---|---|
snpOS00933 | Chr04 | 29670314 | qRCA4 | T/A | T | 98.94 | 4.3 | 6.5 |
snpOS00934 | Chr04 | 29943687 | qRCA4 | A/C | A | 97.87 | 8.7 | 3.2 |
snpOS00935 | Chr04 | 30001385 | qRCA4 | C/T | C | 100.00 | 8.7 | 0.0 |
snpOS00937 | Chr04 | 30193259 | qRCA4 | G/C | G | 100.00 | 8.7 | 0.0 |
snpOS00938 | Chr04 | 30302635 | qRCA4 | A/G | A | 98.94 | 8.7 | 0.0 |
snpOS00940 | Chr04 | 30420667 | qRCA4 | A/C | A | 100.00 | 8.7 | 0.0 |
snpOS00941 | Chr04 | 30461857 | qRCA4 | T/A | T | 100.00 | 8.7 | 0.0 |
snpOS00942 | Chr04 | 30524467 | qRCA4 | A/G | A | 100.00 | 8.7 | 0.0 |
snpOS00944 | Chr04 | 30764226 | qRCA4 | G/A | G | 98.94 | 8.7 | 0.0 |
snpOS00945 | Chr01 | 39443793 | qRCA1.1 | G/A | A | 100.00 | 4.5 | 3.1 |
snpOS00947 | Chr02 | 27974625 | qRCA2.1 | C/T | T | 100.00 | 9.5 | 6.1 |
snpOS00948 | Chr02 | 30450101 | qRCA2.2 | A/T | A | 100.00 | 3.3 | 8.3 |
Population | N | LOD | AE | DE | R2 |
---|---|---|---|---|---|
IRLA | 196 | 4.13 | −5.87 | −1.02 | 9.25 |
IRRL | 331 | 13.63 | −7.56 | −4.08 | 17.27 |
IRNO | 283 | NS | NS | NS | NS |
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Vinarao, R.; Proud, C.; Snell, P.; Fukai, S.; Mitchell, J. QTL Validation and Development of SNP-Based High Throughput Molecular Markers Targeting a Genomic Region Conferring Narrow Root Cone Angle in Aerobic Rice Production Systems. Plants 2021, 10, 2099. https://doi.org/10.3390/plants10102099
Vinarao R, Proud C, Snell P, Fukai S, Mitchell J. QTL Validation and Development of SNP-Based High Throughput Molecular Markers Targeting a Genomic Region Conferring Narrow Root Cone Angle in Aerobic Rice Production Systems. Plants. 2021; 10(10):2099. https://doi.org/10.3390/plants10102099
Chicago/Turabian StyleVinarao, Ricky, Christopher Proud, Peter Snell, Shu Fukai, and Jaquie Mitchell. 2021. "QTL Validation and Development of SNP-Based High Throughput Molecular Markers Targeting a Genomic Region Conferring Narrow Root Cone Angle in Aerobic Rice Production Systems" Plants 10, no. 10: 2099. https://doi.org/10.3390/plants10102099