Maternal Nutrition Affects Nitrogen Isotopic Signature in Blood Plasma of Beef Cattle Dams and Their Offspring
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design and Management
2.2. Bromatological Analysis of Supplements and Pastures
2.3. Processing of Forage and Supplements for the Isotopic Analysis
2.4. Blood Plasma Collection and Processing
2.5. Isotopic Analysis of Delta 15 N (15 N/14 N)
2.6. Statistical Analysis
3. Results
3.1. Isotopic Evaluation of Forages and Supplements
3.2. Isotopic Evaluation of Blood Plasma of Cows
3.3. Isotopic Evaluation of Blood Plasma from Calves
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ingredients | Mineral Supplement | Energetic–Proteic Supplement |
---|---|---|
Ground corn (%) Soybean meal (%) Dicalcium phosphate (%) Urea 45% (%) Salt (%) Minerthal 160 MD (%) * Total digestible nutrients (%) Crude protein (%) Non-protein nitrogen (%) Acid detergent fiber (%) Neutral detergent fiber (%) Fat (%) Calcium (g/kg) Phosphate (g/kg) | 35 - 10 - 30 25 26.76 2.79 - 1.25 4.29 1.26 74.11 59.38 | 60 30 - 2.5 5 2.5 67.55 24.78 7.03 4.76 11.24 2.61 6.2 7.24 |
Forage Availability | Diets | ||
---|---|---|---|
NP | PP | FP | |
Pasture Availability (kg DM/ha) | 3476.24 ± 1594.40 | 4597.35 ± 1189.80 | 5578.03 ± 2049.37 |
Leaf Availability (kg DM/ha) | 573.59 ± 340.56 | 569.13 ± 485.76 | 727.49 ± 643.30 |
Thatch Availability (kg DM/ha) | 562.46 ± 396.97 | 799.05 ± 545.80 | 1347.44 ± 1038.42 |
Dead material availability (kg DM/ha) | 2340.07 ± 1275.00 | 3229.43 ± 973.34 | 3503.07 ± 1410.43 |
Stocking Rate (AU/ha) | 2.19 ± 1.02 | 1.74 ± 0.56 | 2.26 ± 0.99 |
Leaf supply for animal unit (KG/DM) | 316.64 ± 216.96 | 359.93 ± 245.10 | 366.59 ± 306.58 |
Forage Nutrientes (%) | Diets | ||
---|---|---|---|
NP | PP | FP | |
Crude protein | 7.38 ± 1.72 | 7.82 ± 2.28 | 7.40 ± 2.30 |
Total digestible nutrients | 63.07 ± 1.45 | 64.10 ± 2.33 | 61.43 ± 2.12 |
Neutral detergent fiber | 59.03 ± 3.67 | 61.43 ± 5.05 | 58.49 ± 4.11 |
Calcium | 0.38 ± 0.11 | 0.35 ± 0.05 | 0.39 ± 0.08 |
Phosphor | 0.19 ± 0.03 | 0.19 ± 0.03 | 0.17 ± 0.03 |
Traits | Period | Diets | p-Value | ||
---|---|---|---|---|---|
NP | PP | FP | |||
Abundance | Initial | 2.002 ± 0.086 A | 2.107 ± 0.151 A | 2.160 ± 0.078 A | 0.341 |
Pre | 2.412 ± 0.104 B a | 3.390 ± 0.135 B b | 3.796 ± 0.157 B b | <0.001 | |
Post | 2.156 ± 0.073 A B a | 2.722 ± 0.087 C b | 3.094 ± 0.090 C c | <0.001 | |
p-value | 0.033 | <0.001 | <0.001 | ||
Concentration | Initial | 1.204 ± 0.034 A B | 1.192 ± 0.027 | 1.190 ± 0.073 | 0.986 |
Pre | 1.120 ± 0.022 A | 1.270 ± 0.080 | 1.246 ± 0.060 | 0.064 | |
Post | 1.254 ± 0.038 B | 1.280 ± 0.049 | 1.338 ± 0.056 | 0.207 | |
p-value | 0.037 | 0.518 | 0.275 |
Traits | Age | Diets | p-Value | ||
---|---|---|---|---|---|
NP | PP | FP | |||
Abundance | 30 days | 3.022 ± 0.149 a | 3.910 ± 0.095 b | 3.954 ± 0.150 b | 0.002 |
180 days | 3.028 ± 0.228 | 3.385 ± 0.221 | 3.134 ± 0.223 | 0.878 | |
p-value | 1.000 | 0.368 | 0.023 | ||
Concentration | 30 days | 1.082 ± 0.048 | 1.105 ± 0.022 | 1.180 ± 0.042 | 0.479 |
180 days | 1.090 ± 0.022 | 1.137 ± 0.049 | 1.178 ± 0.025 | 0.386 | |
p-value | 1.000 | 0.991 | 1.000 |
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Meciano, G.d.P.; Schalch Junior, F.J.; Polizel, G.H.G.; Fernandes, A.C.; Cracco, R.C.; Saran Netto, A.; Gomes, R.d.C.; Cônsolo, N.R.B.; Santana, M.H.d.A. Maternal Nutrition Affects Nitrogen Isotopic Signature in Blood Plasma of Beef Cattle Dams and Their Offspring. Metabolites 2022, 12, 1249. https://doi.org/10.3390/metabo12121249
Meciano GdP, Schalch Junior FJ, Polizel GHG, Fernandes AC, Cracco RC, Saran Netto A, Gomes RdC, Cônsolo NRB, Santana MHdA. Maternal Nutrition Affects Nitrogen Isotopic Signature in Blood Plasma of Beef Cattle Dams and Their Offspring. Metabolites. 2022; 12(12):1249. https://doi.org/10.3390/metabo12121249
Chicago/Turabian StyleMeciano, Gabriela de Pauli, Fernando José Schalch Junior, Guilherme Henrique Gebim Polizel, Arícia Christofaro Fernandes, Roberta Cavalcante Cracco, Arlindo Saran Netto, Rodrigo da Costa Gomes, Nara Regina Brandão Cônsolo, and Miguel Henrique de Almeida Santana. 2022. "Maternal Nutrition Affects Nitrogen Isotopic Signature in Blood Plasma of Beef Cattle Dams and Their Offspring" Metabolites 12, no. 12: 1249. https://doi.org/10.3390/metabo12121249