The Gut-Ex-Vivo System (GEVS) Is a Dynamic and Versatile Tool for the Study of DNBS-Induced IBD in BALB/C and C57BL/6 Mice, Highlighting the Protective Role of Probiotics
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Materials
2.2. Silicone-Based Device and Colon Culture
2.3. Colon Cultures and Treatments
2.4. Probiotics Formulations and Treatments
2.5. Quantitative PCR (qPCR)
2.6. Western Blotting Analysis
2.7. Immunofluorescence Analysis
2.8. ELISA
2.9. Statistical Analysis
3. Results
3.1. DNBS Triggers UC in the Colon of C57BL/6 Mice by Using a Gut-Ex-Vivo System (GEVS)
3.2. Apoptotic and Ferroptotic Cell Death Modalities Associated with DNBS-Induced UC Can Be Studied by Using a GEVS, in Colon from Both C57BL/6 and BALB/c Mice
3.3. Probiotic Administration Prevents IBD Onset
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ATF4_F | GTTTAGAGCTAGGCAGTGAAG |
ATF4_R | CCTTTACACATGGAGGGATTAG |
ATF6_F | GATGGTGACAACCAGAAAGA |
ATF6_R | TGGAGGTGGAGGCATATAA |
XBP1_F | AGTCCGCAGCAGGTG |
XBP1_R | GGTCCAACTTGTCCAGAATG |
CLD2_F | CCTCGCTGGCTTGTATTATC |
CLD2_R | AAAGACTCCACCCACTACA |
CLD15_F | GGGACCCTCCACATACTT |
CLD15_R | CATACTTGGTTCCAGCATACA |
OCL_F | TCTTTGGAGGAAGCCTAAAC |
OCL_R | CTGCTCTTGGGTCTGTATATC |
IFNγ_F | CCACATCTATGCCACTTGAG |
IFNγ_R | CTCTTCCTCATGGCTGTTTC |
TNFα_F | CCTATGTCTCAGCCTCTTCT |
TNFα_R | GGGAACTTCTCATCCCTTTG |
IL-10_F | TGAATTCCCTGGGTGAGA |
IL-10_R | CCACTGCCTTGCTCTTATT |
BAX_F | GGTTGCCCTCTTCTACTTTG |
BAX_R | AGTGTCCAGCCCATGAT |
PUMA_F | GGTTGCCCTCTTCTACTTTG |
PUMA_R | AGTGTCCAGCCCATGAT |
NOXA_F | GGTTGCCCTCTTCTACTTTG |
NOXA_R | AGTGTCCAGCCCATGAT |
CHAC1_F | TCACAGCACTGGCCTAT |
CHAC1_R | CAAGGTTGTGACCAGAGAAG |
PTGS2_F | GCCTGGTCTGATGATGTATG |
PTGS2_R | GTCTGCTGGTTTGGAATAGT |
ACSL4_F | TAAGCCCAXTTCAGACAAAC |
ACSL4_R | GGCTACAGCATGGTCAAATA |
TFR1_F | GGGCTATTGTAAGCGTGTAG |
TFR1_R | CCTCTGTTTCCATGGTTTCT |
GAPDH_F | TTCAACGGCACAGTCAAG |
GAPDH_R | CCAGTAGACTCCACGACATA |
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Monzani, R.; Gagliardi, M.; Clemente, N.; Saverio, V.; Pańczyszyn, E.; Santoro, C.; Yissachar, N.; Visciglia, A.; Pane, M.; Amoruso, A.; et al. The Gut-Ex-Vivo System (GEVS) Is a Dynamic and Versatile Tool for the Study of DNBS-Induced IBD in BALB/C and C57BL/6 Mice, Highlighting the Protective Role of Probiotics. Biology 2022, 11, 1574. https://doi.org/10.3390/biology11111574
Monzani R, Gagliardi M, Clemente N, Saverio V, Pańczyszyn E, Santoro C, Yissachar N, Visciglia A, Pane M, Amoruso A, et al. The Gut-Ex-Vivo System (GEVS) Is a Dynamic and Versatile Tool for the Study of DNBS-Induced IBD in BALB/C and C57BL/6 Mice, Highlighting the Protective Role of Probiotics. Biology. 2022; 11(11):1574. https://doi.org/10.3390/biology11111574
Chicago/Turabian StyleMonzani, Romina, Mara Gagliardi, Nausicaa Clemente, Valentina Saverio, Elżbieta Pańczyszyn, Claudio Santoro, Nissan Yissachar, Annalisa Visciglia, Marco Pane, Angela Amoruso, and et al. 2022. "The Gut-Ex-Vivo System (GEVS) Is a Dynamic and Versatile Tool for the Study of DNBS-Induced IBD in BALB/C and C57BL/6 Mice, Highlighting the Protective Role of Probiotics" Biology 11, no. 11: 1574. https://doi.org/10.3390/biology11111574