Concordance between Antimicrobial Resistance Phenotype and Genotype of Staphylococcus pseudintermedius from Healthy Dogs
Abstract
:1. Introduction
2. Results
2.1. Distribution of MRSP and MDR Strains
2.2. Genotype and Phenotype Concordance Varied Depending on the Antibiotic Family
2.3. Beta-Lactams and Cephalosporin Resistance
2.4. Fluoroquinolone Resistance
2.5. Vancomycin Resistance
2.6. Macrolide and Lincosamide Resistance
2.7. Tetracycline Resistance
2.8. Trimethoprim-Sulfamethoxazole (Cotrimoxazole) Resistance
2.9. Aminoglycoside Resistance
2.10. Genes Not Tested Phenotypically: sat-4 and cat
3. Discussion
4. Materials and Methods
4.1. Bacterial Cultures
4.2. Antibiotic Susceptibility
4.3. DNA Extraction and Nanopore Sequencing Library Preparation
4.4. Bioinformatic Genome Sequencing Analysis
4.5. Antibiotic Resistance Bioinformatic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dog | Inguinal | Perianal | Perioral | Nasal | Total |
---|---|---|---|---|---|
1 | 2 | 5 | 5 | 1 | 13 |
2 | 0 | 1 | 0 | 0 | 1 |
3 | 0 | 1 | 4 | 0 | 5 |
4 | 0 | 0 | 0 | 2 | 2 |
5 | 0 | 1 | 5 | 0 | 6 |
6 | 4 | 3 | 5 | 5 | 17 |
7 | 5 | 0 | 5 | 1 | 11 |
8 | 2 | 5 | 3 | 0 | 10 |
9 | 0 | 0 | 0 | 2 | 2 |
Total | 13 | 16 | 27 | 11 |
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Viñes, J.; Fàbregas, N.; Pérez, D.; Cuscó, A.; Fonticoba, R.; Francino, O.; Ferrer, L.; Migura-Garcia, L. Concordance between Antimicrobial Resistance Phenotype and Genotype of Staphylococcus pseudintermedius from Healthy Dogs. Antibiotics 2022, 11, 1625. https://doi.org/10.3390/antibiotics11111625
Viñes J, Fàbregas N, Pérez D, Cuscó A, Fonticoba R, Francino O, Ferrer L, Migura-Garcia L. Concordance between Antimicrobial Resistance Phenotype and Genotype of Staphylococcus pseudintermedius from Healthy Dogs. Antibiotics. 2022; 11(11):1625. https://doi.org/10.3390/antibiotics11111625
Chicago/Turabian StyleViñes, Joaquim, Norma Fàbregas, Daniel Pérez, Anna Cuscó, Rocío Fonticoba, Olga Francino, Lluís Ferrer, and Lourdes Migura-Garcia. 2022. "Concordance between Antimicrobial Resistance Phenotype and Genotype of Staphylococcus pseudintermedius from Healthy Dogs" Antibiotics 11, no. 11: 1625. https://doi.org/10.3390/antibiotics11111625