Prevalence and Genetic Analysis of Resistance Mechanisms of Linezolid-Nonsusceptible Enterococci in a Tertiary Care Hospital Examined via Whole-Genome Sequencing
Abstract
:1. Introduction
2. Results
2.1. Evolution of Linezolid Nonsusceptibility in Severance Hospital over the Past Five Years
2.2. Linezolid Minimum Inhibitory Concentration (MIC) Distribution and Resistance Mechanisms
2.3. Genetic Environment of Different Resistance Determinants
2.4. Clonal Relatedness and Other Molecular Features
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. Linezolid Susceptibility Testing
4.3. Whole-Genome Sequencing and Bioinformatic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Year | E. faecalis | E. faecium | ||||||
---|---|---|---|---|---|---|---|---|
No. Isoltaes | S a | I b | R c | No. Isoltaes | S | I | R | |
2016 | 1536 | 96.1% | 0.5% | 3.4% | 2121 | 96.4% | 0.1% | 3.5% |
2017 | 1505 | 95.3% | 1.1% | 3.7% | 2407 | 97.6% | 0.2% | 2.2% |
2018 | 1716 | 95.4% | 1.4% | 3.2% | 2558 | 96.9% | 0.4% | 2.7% |
2019 | 1544 | 94.2% | 1.2% | 4.7% | 2606 | 95.6% | 0.7% | 3.8% |
2020 | 1366 | 90.7% | 2.6% | 6.7% | 2559 | 94.6% | 0.4% | 5.0% |
MIC (μg/mL) | 4 | 8 | 16 | 32 |
---|---|---|---|---|
E. faecalis (n = 18) | 4 (22.2) | 14 (77.8) | 0 | 0 |
optrA (n = 15, 83.3%) | 1 (6.7) | 14 (93.3) | ||
cfr(D) + poxtA2 (n = 3, 16.7%) | 3 (100) | |||
E. faecium (n = 14) | 1 (7.1) | 7 (50.0) | 3 (21.4) | 3 (21.4) |
optrA (n = 2, 14.3%) | 2 (100) | |||
optrA+cfr(D) + poxtA-EF9F6 (n = 1, 7.1%) | 1 (100) | |||
cfr(D) + poxtA-EF9F6 (n = 1, 7.1%) | 1 (100) | |||
cfr(D) + poxtA2 (n = 2, 14.3%) | 1 (50.00) | 1 (50.0) | ||
G2576T (n = 4, 28.6%) | 1 (25.0) | 1 (25.0) | 2 (50.0) | |
unknown mechanism (n = 4, 28.6%) | 1 (25.0) | 2 (50.0) | 1 (25.0) | |
E. gallinarum (n = 2) | 0 | 2 (100) | 0 | 0 |
poxtA-EF9F6 (n = 2, 100%) | 2 (100) | |||
Total (n = 34) | 5 (14.7) | 23 (67.6) | 3 (8.8) | 3 (8.8) |
optrA (n = 17, 50.0%) | 1 (5.9) | 16 (94.1) | ||
optrA+cfr(D) + poxtA-EF9F6 (n = 1, 2.9%) | 1 (100) | |||
cfr(D) + poxtA-EF9F6 (n = 1, 2.9%) | 1 (100) | |||
cfr(D) + poxtA2 (n = 5, 14.7%) | 4 (80.0) | 1 (20.0) | ||
poxtA-EF9F6 (n = 2, 5.9%) | 2 (100) | |||
G2576T (n = 4, 11.8%) | 1 (25.0) | 1 (25.0) | 2 (50.0) | |
unknown mechanism (n = 4, 11.8%) | 1 (25.0) | 2 (50.0) | 1 (25.0) |
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Hu, Y.; Won, D.; Nguyen, L.P.; Osei, K.M.; Seo, Y.; Kim, J.; Lee, Y.; Lee, H.; Yong, D.; Choi, J.R.; et al. Prevalence and Genetic Analysis of Resistance Mechanisms of Linezolid-Nonsusceptible Enterococci in a Tertiary Care Hospital Examined via Whole-Genome Sequencing. Antibiotics 2022, 11, 1624. https://doi.org/10.3390/antibiotics11111624
Hu Y, Won D, Nguyen LP, Osei KM, Seo Y, Kim J, Lee Y, Lee H, Yong D, Choi JR, et al. Prevalence and Genetic Analysis of Resistance Mechanisms of Linezolid-Nonsusceptible Enterococci in a Tertiary Care Hospital Examined via Whole-Genome Sequencing. Antibiotics. 2022; 11(11):1624. https://doi.org/10.3390/antibiotics11111624
Chicago/Turabian StyleHu, Yuxin, Dongju Won, Le Phuong Nguyen, Kennedy Mensah Osei, Younghee Seo, Junglim Kim, Yoonhee Lee, Hyukmin Lee, Dongeun Yong, Jong Rak Choi, and et al. 2022. "Prevalence and Genetic Analysis of Resistance Mechanisms of Linezolid-Nonsusceptible Enterococci in a Tertiary Care Hospital Examined via Whole-Genome Sequencing" Antibiotics 11, no. 11: 1624. https://doi.org/10.3390/antibiotics11111624