Buspirone Enhances Cell Survival and Preserves Structural Integrity during Oxidative Injury to the Retinal Pigment Epithelium
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Cellular Apoptosis Assay
2.3. Animals
2.4. Real Time PCR
2.5. SD-OCT Imaging and Measurement of Total Retinal Thickness
2.6. Immunohistochemistry
2.7. Statistical Analysis
3. Results
3.1. Buspirone Prevented Oxidative Stress Induced Cell Death
3.2. Buspirone Prevented RPE Structural Disintegration from Oxidative Damage
3.3. Presence of Buspirone-Preserved Retinal Structure in an Acute Model of RPE Oxidative Damge
3.4. Buspirone Preserved RPE Structural Integrity In Vivo from Oxidative Damage
3.5. Increased Expression of Antioxidant Genes in the RPE/Choroid in Response to Buspirone Treatment
4. Discussion
4.1. Buspirone’s Multifaceted Protective Effects in Retinal Health
4.2. Potential Mechanisms Underlying Buspirone’s Protective Effects
4.3. Clinical Implications and Future Directions
4.4. Limitations of the Study
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Ref. Seq | Forward Primer | Reverse Primer |
---|---|---|---|
Beta-Actin | NM_007393.3 | CGAGCACAGCTTCTTTGCAG | TTCCCACCATCACACCCTGG |
Nqo1 | NM_008706.5 | CGACAACGGTCCTTTCCAGA | CCAGACGGTTTCCAGACGTT |
Cat | NM_009804.2 | CGCAATCCTACACCATGTCG | AGTATCCAAAAGCACCTGCTCC |
Sqstm1 | NM_011018.2 | GGAAGCTGCCCTATACCCAC | GCTTGGCCACAGCACTATCA |
Gstm1 | NM_010358.5 | GGGATACTGGAACGTCCGC | GCTCTGGGTGATCTTGTGTGA |
Sod2 | NM_013671.3 | CAGGATGCCGCTCCGTTAT | TGAGGTTTACACGACCGCTG |
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Biswal, M.R.; Paulson, R.J.; Vichare, R.; Lewin, A.S. Buspirone Enhances Cell Survival and Preserves Structural Integrity during Oxidative Injury to the Retinal Pigment Epithelium. Antioxidants 2023, 12, 2129. https://doi.org/10.3390/antiox12122129
Biswal MR, Paulson RJ, Vichare R, Lewin AS. Buspirone Enhances Cell Survival and Preserves Structural Integrity during Oxidative Injury to the Retinal Pigment Epithelium. Antioxidants. 2023; 12(12):2129. https://doi.org/10.3390/antiox12122129
Chicago/Turabian StyleBiswal, Manas R., Ryan J. Paulson, Riddhi Vichare, and Alfred S. Lewin. 2023. "Buspirone Enhances Cell Survival and Preserves Structural Integrity during Oxidative Injury to the Retinal Pigment Epithelium" Antioxidants 12, no. 12: 2129. https://doi.org/10.3390/antiox12122129