Oxymatrine Improves Oxidative Stress-Induced Senescence in HT22 Cells and Mice via the Activation of AMP-Activated Protein Kinase
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. HT22 Cell Culture
2.3. Cell Viability Assay
2.4. Detection of Intracellular ROS
2.5. Immunoblotting Analysis
2.6. Animals and Administration of Drugs
2.7. Open Field Test
2.8. Morris Water Maze Test
2.9. RNA Extraction and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR)
2.10. Statistical Analyses
3. Results
3.1. Oxymatrine Protects the HT22 Cells against Oxidative Stress
3.2. OM Reduces ROS Caused by Oxidative Stress in HT22 Cells
3.3. OM Reduces Oxidative Stress-Induced Senescence via the Activation of AMPK in HT22 Cells
3.4. Oxymatrine Improves Behavioral Dysfunction in D-Gal-Induced Senescence Mice
3.5. Oxymatrine Improved Locomotor Activity and Reduced Anxiety Symptoms in D-Gal-Induced Senescence Mice
3.6. Oxymatrine Reduces RAGE Expression and Hippocampal Senescence via the Activation of AMPK in D-Galactose-Induced Senescence Mice
3.7. Oxymatrine Reduces Senescence via the Activation of AMPK and Autophagy in D-Gal-Induced Senescence Mice Heart Tissue
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Maharajan, N.; Lee, C.-M.; Vijayakumar, K.A.; Cho, G.-W. Oxymatrine Improves Oxidative Stress-Induced Senescence in HT22 Cells and Mice via the Activation of AMP-Activated Protein Kinase. Antioxidants 2023, 12, 2078. https://doi.org/10.3390/antiox12122078
Maharajan N, Lee C-M, Vijayakumar KA, Cho G-W. Oxymatrine Improves Oxidative Stress-Induced Senescence in HT22 Cells and Mice via the Activation of AMP-Activated Protein Kinase. Antioxidants. 2023; 12(12):2078. https://doi.org/10.3390/antiox12122078
Chicago/Turabian StyleMaharajan, Nagarajan, Chang-Min Lee, Karthikeyan A. Vijayakumar, and Gwang-Won Cho. 2023. "Oxymatrine Improves Oxidative Stress-Induced Senescence in HT22 Cells and Mice via the Activation of AMP-Activated Protein Kinase" Antioxidants 12, no. 12: 2078. https://doi.org/10.3390/antiox12122078