Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Cell Culture
2.3. Cell Viability Assay
2.4. Determination of Lactate Dehydrogenase (LDH), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT) Activity
2.5. RNA-Seq Analysis
2.6. Oxidative Stress Analysis
2.7. Mitochondrial Membrane Potential Determination
2.8. Quantitative Real-Time PCR
2.9. Western Blotting
2.10. Molecular Docking of CGA with the KELCH-like ECH-Associated Protein1 (Keap-1)-Nrf2 Complex
2.11. Statistical Analyses
3. Results
3.1. CGA Attenuated AFB1-Induced L-02 Cell Cytotoxicity
3.2. RNA-Seq Analysis
3.3. CGA Alleviated AFB1-Induced Oxidative Damage by Activating Nrf2/Heme Oxygenase-1 (HO-1) Signaling Pathway in L-02 Cells
3.4. CGA Alleviated AFB1-Induced Inflammatory Response by Preventing Noncanonical Nuclear Factor Kappa-B (NF-κB) Pathway in L-02 Cells
3.5. CGA Alleviated AFB1-Induced Apoptosis in L-02 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Gene Accession Number |
---|---|---|---|
Caspase-3 | CCAAAGATCATACATGGAAGCG | CTGAATGTTTCCCTGAGGTTTG | XM_054350958.1 |
Caspase-8 | CAAACTTCACAGCATTAGGGAC | ATGTTACTGTGGTCCATGAGTT | NM_033355.4 |
Caspase-9 | TCCAGGAAGGTTTGAGGACC | CCCTTTCACCGAAACAGCAT | XM_011542273.4 |
Bax | CCCGAGAGGTCTTTTTCCGAG | CCAGCCCATGATGGTTCTGAT | XM_047439168.1 |
Bcl-2 | GACTTCGCCGAGATGTCCAG | GAACTCAAAGAAGGCCACAATC | XM_054318967.1 |
GAPDH | CTCTGCTCCTCCTGTTCGAC | TTAAAAGCAGCCCTGGTGAC | NM_001357943.2 |
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Wang, Q.; Liu, T.; Koci, M.; Wang, Y.; Fu, Y.; Ma, M.; Ma, Q.; Zhao, L. Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway. Antioxidants 2023, 12, 2027. https://doi.org/10.3390/antiox12122027
Wang Q, Liu T, Koci M, Wang Y, Fu Y, Ma M, Ma Q, Zhao L. Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway. Antioxidants. 2023; 12(12):2027. https://doi.org/10.3390/antiox12122027
Chicago/Turabian StyleWang, Qianqian, Tianxu Liu, Matthew Koci, Yanan Wang, Yutong Fu, Mingxin Ma, Qiugang Ma, and Lihong Zhao. 2023. "Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway" Antioxidants 12, no. 12: 2027. https://doi.org/10.3390/antiox12122027