Paradoxical Radiosensitizing Effect of Carnosic Acid on B16F10 Metastatic Melanoma Cells: A New Treatment Strategy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Carnosic Acid
2.2. Antigenototoxic Effect
2.2.1. In Vivo Micronucleus Assay
- Micronucleus Assay in Mouse Bone Marrow (MNPCEs)
- Flow Cytometry for Micronuclei (MN) in Reticulocytes (MNRET)
2.2.2. In Vitro Micronucleus Assay
- Micronucleus Test in Vitro (MNCB)
2.3. MTT Cytotoxicity Assay
MTT Assays of Irradiated Cells
2.4. Clonogenic Assay
2.5. Anexin V
2.6. GSH Assay
2.7. Models of Radiation-Induced Bystander Effects (RIBE)
2.8. Irradiation
2.9. Statistical Analysis
3. Results
3.1. Antigenototoxic Effect
3.1.1. In Vivo Micronucleus Assay
- Micronucleus Assay in Mouse Bone Marrow (MNPCEs)
- Flow Cytometry for Micronuclei (MN) in Reticulocytes (RET)
3.1.2. In Vitro Micronucleus Assay
- Micronucleus test in vitro (CBMN)
3.2. Cytotoxicity Assay. MTT Assay
- PNT2 Cells
- B16F10 Cells
3.3. Clonogenic Assay
3.4. Apoptosis
3.5. GSH Assay
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Alcaraz, M.; Olivares, A.; Andreu-Gálvez, M.; Achel, D.G.; Mercado, A.M.; Alcaraz-Saura, M. Paradoxical Radiosensitizing Effect of Carnosic Acid on B16F10 Metastatic Melanoma Cells: A New Treatment Strategy. Antioxidants 2022, 11, 2166. https://doi.org/10.3390/antiox11112166
Alcaraz M, Olivares A, Andreu-Gálvez M, Achel DG, Mercado AM, Alcaraz-Saura M. Paradoxical Radiosensitizing Effect of Carnosic Acid on B16F10 Metastatic Melanoma Cells: A New Treatment Strategy. Antioxidants. 2022; 11(11):2166. https://doi.org/10.3390/antiox11112166
Chicago/Turabian StyleAlcaraz, Miguel, Amparo Olivares, Marina Andreu-Gálvez, Daniel Gyingiri Achel, Ana María Mercado, and Miguel Alcaraz-Saura. 2022. "Paradoxical Radiosensitizing Effect of Carnosic Acid on B16F10 Metastatic Melanoma Cells: A New Treatment Strategy" Antioxidants 11, no. 11: 2166. https://doi.org/10.3390/antiox11112166