Targeted UHPLC-MS Analysis Reveals Disparate Polyphenol Composition and Concentration in Muscadine Grape Supplements with Proportional Antioxidant Activity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Commercially Processed MGSs
2.3. Extraction of Phenolic Compounds
2.4. Total Phenolic Content (TPC)
2.5. Quantification of Antioxidant Capacity
2.6. UHPLC-MS Analysis
2.7. Quantification of Antioxidant Markers and Enzymes
2.8. Data and Statistical Analysis
3. Results
3.1. Total Phenolic Content (TPC) and Antioxidant Capacity of MGSs
3.2. UHPLC-MS of Commercially Processed MGSs
3.3. Polyphenol Content in Commercial MGSs
3.4. Effect of Commercially Prepared MGSs on Oxidative Stress
3.5. Effect of Commercially Prepared MGSs on Antioxidant Enzymes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chappell, M.C.; Duncan, A.V.; Melo, A.C.; Schaich, C.L.; Pirro, N.T.; Diz, D.I.; Tallant, E.A.; Gallagher, P.E. Targeted UHPLC-MS Analysis Reveals Disparate Polyphenol Composition and Concentration in Muscadine Grape Supplements with Proportional Antioxidant Activity. Antioxidants 2022, 11, 2117. https://doi.org/10.3390/antiox11112117
Chappell MC, Duncan AV, Melo AC, Schaich CL, Pirro NT, Diz DI, Tallant EA, Gallagher PE. Targeted UHPLC-MS Analysis Reveals Disparate Polyphenol Composition and Concentration in Muscadine Grape Supplements with Proportional Antioxidant Activity. Antioxidants. 2022; 11(11):2117. https://doi.org/10.3390/antiox11112117
Chicago/Turabian StyleChappell, Mark C., Aja V. Duncan, Ana Clara Melo, Christopher L. Schaich, Nancy T. Pirro, Debra I. Diz, E. Ann Tallant, and Patricia E. Gallagher. 2022. "Targeted UHPLC-MS Analysis Reveals Disparate Polyphenol Composition and Concentration in Muscadine Grape Supplements with Proportional Antioxidant Activity" Antioxidants 11, no. 11: 2117. https://doi.org/10.3390/antiox11112117