Protective Effects of Lignin-Carbohydrate Complexes from Wheat Stalk against Bisphenol a Neurotoxicity in Zebrafish via Oxidative Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Analysis and Characterization of LCC Preparations
2.3. Zebrafish Husbandry
2.4. Effects of LCCs on the Hatching Rate of Zebrafish
2.5. Effects of LCCs on Zebrafish Behavior
2.6. Antioxidant Properties and Effects of LCCs on Oxidative Stress in Zebrafish
2.7. Neuroprotective Effects of LCCs in Zebrafish
2.8. Real-Time PCR
2.9. Statistical Analysis
3. Results and Discussion
3.1. Composition and Molecular Weight of the LCC Preparations
3.2. Structural Properties of the LCC Preparations
3.3. Effects of LCCs on the Survival Rate and Hatching Rate of Zebrafish
3.4. Effects of LCC-WS-A and LCC-WS-B on the Behavior of BPA-Treated Zebrafish
3.5. In Vitro and In Vivo Antioxidant Properties of LCCs and their Protective Effects against BPA-Induced Oxidative Damage in Zebrafish
3.6. Effects of LCC-WS-A and LCC-WS-B on Gene Expression in Tg Zebrafish
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Preparation | Lignin (%) | Cellulose (%) | Hemicellulose (%) | Molecular Weight | Polyphenols (%) | |||||
---|---|---|---|---|---|---|---|---|---|---|
Xylan | Arabinan | Galactan | Mannan | Mw (g·mol−1) | Mn (g·mol−1) | PDI | ||||
LCC-WS-A | 42.5 | 3.4 | 40.5 | 1.9 | 0.2 | 0.1 | 17,600 | 7890 | 2.2 | 3.3 |
LCC-WS-B | 64.2 | 1.6 | 23.5 | 0.8 | 0 | 0 | 9210 | 4520 | 2.0 | 3.1 |
Characteristics | LCC-WS-A | LCC-WS-B |
---|---|---|
Lignin interunit linkages | ||
β-O-4 aryl ethers (A) | 21.9 | 14.1 |
Resinols (B) | 5.5 | - |
Phenylcoumarans, β-5 (C) | 2.3 | - |
S:G ratio a | 0.8 | 1.9 |
LCC linkages | ||
Benzyl ether (BE) | 2.9 | - |
Phenyl glycoside (PhGlc) | 13.5 | 4.3 |
Est b | 6.3 | 5.9 |
Total c | 22.7 | 10.2 |
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Gu, J.; Guo, M.; Zheng, L.; Yin, X.; Zhou, L.; Fan, D.; Shi, L.; Huang, C.; Ji, G. Protective Effects of Lignin-Carbohydrate Complexes from Wheat Stalk against Bisphenol a Neurotoxicity in Zebrafish via Oxidative Stress. Antioxidants 2021, 10, 1640. https://doi.org/10.3390/antiox10101640
Gu J, Guo M, Zheng L, Yin X, Zhou L, Fan D, Shi L, Huang C, Ji G. Protective Effects of Lignin-Carbohydrate Complexes from Wheat Stalk against Bisphenol a Neurotoxicity in Zebrafish via Oxidative Stress. Antioxidants. 2021; 10(10):1640. https://doi.org/10.3390/antiox10101640
Chicago/Turabian StyleGu, Jie, Min Guo, Liping Zheng, Xiaogang Yin, Linjun Zhou, Deling Fan, Lili Shi, Caoxing Huang, and Guixiang Ji. 2021. "Protective Effects of Lignin-Carbohydrate Complexes from Wheat Stalk against Bisphenol a Neurotoxicity in Zebrafish via Oxidative Stress" Antioxidants 10, no. 10: 1640. https://doi.org/10.3390/antiox10101640