Next-Generation Sequencing-Based Monitoring of Intestinal Bacteria and Bacteriophages Following Fecal Microbiota Transplantation in Inflammatory Bowel Diseases
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Statement
2.2. Patients and Study Design
2.3. DNA Isolation and NGS Procedures
2.4. Bioinformatic Analysis of Bacterial 16S rRNA
2.5. Classification of Detected Viral Sequences
3. Results
3.1. Post-FMT Changes for Clinically Relevant Bacteria
3.2. Phageome Changes Following FMT
3.3. Correlations between Bacterial Groups and Detectable Bacteriophages
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameters | Crohn’s Disease (n = 9) | Graft-Versus-Host Disease (n = 6) |
---|---|---|
Gender (males/females) | 5/4 | 4/2 |
Median age, years (min and max values) | 29 (22–59) | 27 (9–59) |
Body mass, kg (min and max values) | 66 (42–69) | 49 (28–70) |
Duration of the primary illness (min and max values) | 4 (1–15) | 6 (2–9) |
Phylum | Class | Genus | Species | Bacteriophage |
---|---|---|---|---|
Uroviricota | Caudoviricetes | Bievrevirus | Bievrevirus bv4A7 | Escherichia phage 4A7 |
Tequatrovirus | Tequatrovirus gee4498 | Escher phage vB_EcoM_G4498 | ||
Traversvirus | Traversvirus II | Escherichia phage Stx2 II | ||
Shamshuipovirus | Shamshuipovirus mEpX2 | Escherichia phage mEpX2 | ||
Eganvirus | NC | Salmonella phage BIS20 | ||
Brunovirus | Brunovirus SEN34 | Salmonella phage SEN34 | ||
Goslarvirus | Goslarvirus goslar | Escherichia phage vB_EcoM_Goslar | ||
Lambdavirus | NC | Enterobacteria phage mEp237 | ||
Lederbergvirus | Lederbergvirus Sf6 | Shigella phage Sf6 | ||
Lederbergvirus | NC | Enterobacter phage IME10 | ||
Lederbergvirus | NC | Salmonella phage SEN22 | ||
Novemvirus | Novemvirus T5282H | Enterobacter phage phiT5282H | ||
Oengusvirus | Oengusvirus oengus | Faecalibacterium phage FP_oengus | ||
Caudoviricetes unclassified | NC | Aeromonas phage pAEv1818 | ||
Uroviricota | Caudoviricetes (Crassvirales) | Cohcovirus | Cohcovirus splanchnicus | uncultured phage cr30_1 |
Kahnovirus | Kahnovirus copri | uncultured phage cr44_1 | ||
Kahnovirus | Kahnovirus oralis | uncultured phage cr85_1 | ||
Birpovirus | Birpovirus hominis | uncultured phage cr19_1 | ||
Jahgtovirus | Jahgtovirus intestinihominis | uncultured phage cr107_1 | ||
Peploviricota | Herviviricetes | Cytomegalovirus | Human betaherpesvirus 5 | NC |
Preplasmiviricota | Tectiliviricetes | Mastadenovirus | Mastadenovirus D | Mastadenovirus 54 (serotype) |
Bacterial Phyla, Genera | Phages (Phyla, Genera, Species) | Correlation Quotients | p Value |
---|---|---|---|
Proteobacteriota | Caudoviricetes | 0.667 | 0.0002 |
Lederbergvirus | 0.477 | 0.009 | |
Enterobacter phage IME10 | 0.552 | 0.004 | |
E. coli phage 4A7 | 0.617 | 0.0065 | |
Salmonella phage SEN34 | 0.538 | 0.003 | |
Aeromonas phage pAEv1818 | 0.535 | 0.004 | |
Genus Faecalibacteria | Bamfordviricota, Preplasmaviricota | 0.730 | 0.00003 |
Clostridia_ss | Peploviricota | 0.660 | 0.0002 |
Klebsiella | Lederbergvirus | 0.703 | 0.00006 |
Enterobacter phage IME10 | 0.625 | 0.0055 | |
E. coli phage HK106 | 0.567 | 0.002 | |
Traversvirus | 0.526 | 0.004 | |
Salmonella phage SEN34 | 0.564 | 0.002 | |
Aeromonas phage pAEv1818 | 0.561 | 0.002 | |
Enterococcus | Caudoviricetes | 0.630 | 0.0005 |
E. coli phage 4A7 | 0.479 | 0.009 | |
Salmonella phage SEN34 | 0.489 | 0.007 | |
Aeromonas phage pAEv1818 | 0.504 | 0.006 |
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Goloshchapov, O.V.; Shchukina, O.B.; Kusakin, A.V.; Tsai, V.V.; Kalinin, R.S.; Eismont, Y.A.; Glotov, O.S.; Chukhlovin, A.B. Next-Generation Sequencing-Based Monitoring of Intestinal Bacteria and Bacteriophages Following Fecal Microbiota Transplantation in Inflammatory Bowel Diseases. Pathogens 2023, 12, 1438. https://doi.org/10.3390/pathogens12121438
Goloshchapov OV, Shchukina OB, Kusakin AV, Tsai VV, Kalinin RS, Eismont YA, Glotov OS, Chukhlovin AB. Next-Generation Sequencing-Based Monitoring of Intestinal Bacteria and Bacteriophages Following Fecal Microbiota Transplantation in Inflammatory Bowel Diseases. Pathogens. 2023; 12(12):1438. https://doi.org/10.3390/pathogens12121438
Chicago/Turabian StyleGoloshchapov, Oleg V., Oksana B. Shchukina, Aleksey V. Kusakin, Viktoria V. Tsai, Roman S. Kalinin, Yury A. Eismont, Oleg S. Glotov, and Alexei B. Chukhlovin. 2023. "Next-Generation Sequencing-Based Monitoring of Intestinal Bacteria and Bacteriophages Following Fecal Microbiota Transplantation in Inflammatory Bowel Diseases" Pathogens 12, no. 12: 1438. https://doi.org/10.3390/pathogens12121438