Genetic Characterization of the Poultry Red Mite (Dermanyssus gallinae) in Poland and a Comparison with European and Asian Isolates
Abstract
:1. Introduction
2. Materials and Methods
2.1. Dermanyssus gallinae
2.2. Isolation of Genomic DNA
2.3. PCR Assay
2.4. Sequencing
2.5. Phylogenetic Analysis
2.5.1. Consensus Sequences
2.5.2. Homologous Sequences
2.5.3. Sequence Alignment Analysis
2.5.4. Haplotype Analysis
2.5.5. Generation of a Phylogenetic Tree
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Number of Haplotypes | Sequence (GenBank ID) | Country | Ref. |
---|---|---|---|---|
1 | 34 | MK599418 | Japan | |
LC029558, LC029544, LC029537, LC029535, LC029534, LC029533, LC029531, LC029528, LC029524, LC029523, LC029522, LC029521, LC029515, LC029513, LC029512, LC029511, LC029505, LC029494, LC029492, LC029491, LC029488, LC029483, LC029481, LC029480, LC029479, LC029477, LC029476, LC029473, LC029472, LC029469, LC029468, LC029464, LC029460, | Japan | [17] | ||
2 | 14 | LC029542, LC029536, LC029504, LC029501, LC029499, LC029493, LC029489, LC029487, LC029482, LC029474, LC029471, LC029470, LC029466, LC029463, | Japan | [17] |
3 | 1 | LC029529 | Japan | [17] |
4 | 1 | LC029467 | Japan | [17] |
5 | 2 | LC029540, LC029503 | Japan | [17] |
6 | 2 | LC029520, LC029462 | Japan | [17] |
7 | 2 | LC029507, LC029478 | Japan | [17] |
8 | 1 | LC029500 | Japan | [17] |
9 | 1 | LC029458 | Japan | [17] |
10 | 1 | FM208732 | France | [12] |
11 | 1 | LC029553 | Japan | [17] |
12 | 13 | LC029551, LC029550, LC029549, LC029548, LC029547, LC029526, LC029519, LC029518, LC029517, LC029497, LC029496, | Japan | [17] |
LR812399 | Greece | [23] | ||
LR812378 | Great Britain | [23] | ||
13 | 1 | LC029502 | Japan | [17] |
14 | 1 | LC029465 | Japan | [17] |
15 | 1 | LC029525 | Japan | [17] |
16 | 1 | AM921857 | France | [12] |
17 | 1 | AM921853 | Norway | [12] |
18 | 1 | FM208722 | France | [12] |
19 | 1 | FM207497 | Netherlands | [12] |
20 | 1 | FM207495 | Netherlands | [12] |
21 | 1 | AM921858 | France | [12] |
22 | 1 | FM208739 | France | [12] |
23 | 1 | AM921856 | Denmark | [12] |
24 | 1 | AM921863 | France | [12] |
25 | 1 | FM207498 | Netherlands | [12] |
26 | 1 | AM921865 | France | [12] |
27 | 50 | LR812452, LR812451, LR812450, LR812449, LR812448, LR812447, LR812433, LR812432, LR812431, LR812430, LR812429, LR812428, LR812427, LR812418, LR812417, LR812416, LR812415, LR812414, LR812413, LR812412, LR812411, LR812410, LR812409, LR812408, LR812407, LR812405, LR812404, LR812403, LR812402, LR812398, LR812397, LR812396, LR812395, LR812394, LR812393, LR812392, LR812391, | Greece | [23] |
LR812406 | Slovenia | [23] | ||
LR812390, LR812373, LR812372, LR812371, LR812370, LR812369, | Great Britain | [23] | ||
LR812346, LR812345, LR812343, LR812342, LR812341, LR812340, | Romania | [23] | ||
LR812406, LR812406, LR812406 | Slovenia | [23] | ||
28 | 1 | LR812383 | Great Britain | [23] |
29 | 1 | LR812375 | Great Britain | [23] |
30 | 3 | LR812339, LR812333, LR812332, | Belgium | [23] |
31 | 3 | LR812312, LR812311, LR812310 | Portugal | [23] |
32 | 2 | LR812290, LR812289 | Albania | [23] |
33 | 3 | LR812477, LR812377, LR812376.1 | Great Britain | [23] |
34 | 5 | LR812476, LR812475, LR812474, LR81247, LR812472 | Great Britain | [23] |
35 | 1 | LR812434 | Greece | [23] |
36 | 1 | LR812374 | Great Britain | [23] |
37 | 1 | LC029552 | Japan | [17] |
38 | 1 | LR812317 | Czechia | [23] |
39 | 1 | LR812420 | Greece | [23] |
40 | 5 | LR812351, LR812350, LR812349, LR812348, LR812347 | Turkey | [23] |
41 | 19 | LC029557, LC029556, LC029554, LC029546, LC029545, LC029541, LC029532, LC029527, LC029514, LC029510, LC029509, LC029506, LC029498, LC029490, LC029486, LC029485, LC029475, LC029459, LC029457, | Japan | [17] |
42 | 1 | LC029484 | Japan | [17] |
43 | 2 | AM921852 | Norway | [12] |
LR812361 | Netherlands | [23] | ||
44 | 27 | LR812446, LR812445, LR812444, LR812443, LR812442, LR812441, LR812440, LR812439, LR812438, LR812437, LR812436, LR812435, LR812426, LR812425, LR812424, LR812423, LR812422, LR812421, LR812419, LR812401, LR812400 | Netherlands | [23] |
LR812385, LR812384, LR812381, LR812380 LR812379 | Great Britain | [23] | ||
LR812367 | Netherlands | [23] | ||
45 | 1 | LR812352 | Turkey | [23] |
46 | 55 | MN249083, MN249082, MN249080, MN249079, MN249078, MN249077, MN249076, MN249075, MN249074, MN249073, MN249072 | South Korea | [24] |
LC029538, LC029516, LC029508, LC029495 | Japan | [17] | ||
AM921854 | Poland | [12] | ||
LR812388 | Great Britain | [23] | ||
LR812363, LR812362 | Netherlands | [23] | ||
LR812337, LR812336 | Belgium | [23] | ||
A1-A5, B1, B3-9, C1-5, D 1-6, D8-10, E1-7 (OL547403-436) | Poland | |||
47 | 2 | LC029543, LC029461 | Japan | [17] |
48 | 1 | LC029539 | Japan | [17] |
49 | 1 | LR812334 | Belgium | [23] |
50 | 1 | MT232060 | Albania | |
51 | 2 | LC029555, LC029530 | Japan | [17] |
52 | 2 | FM208717 | Belgium | [12] |
LR812470 | Great Britain | [23] | ||
53 | 1 | LR812344 | Romania | [23] |
54 | 1 | MT232059 | Albania | |
55 | 1 | LR812321 | Czechia | [23] |
56 | 2 | LR812468 LR812467 | Great Britain | [23] |
57 | 4 | AM921864 | France | [12] |
LR812307 | Portugal | [23] | ||
LR812458, LR812455 | Italy | [23] | ||
58 | 2 | LR812319, LR812318 | Czechia | [23] |
59 | 1 | LR812288 | Albania | [23] |
60 | 8 | LR812469, LR812466, LR812465, LR812464, LR812463, LR812462, LR812387, LR812386 | Great Britain | [23] |
61 | 1 | LR812389 | Great Britain | [23] |
62 | 1 | LR812335 | Belgium | [23] |
63 | 22 | MT230034, MT230032, KY025552 | Italy | |
FM208725, FM208718, FM208719, FM208733 | France | [12] | ||
LR812301, LR812300, LR812299, LR812298, | France | [23] | ||
LR812296, LR812294, LR812293 | Croatia | [23] | ||
LR812460.1, LR812459, LR812457, LR812456, LR812454, LR812453 | Italy | [23] | ||
LR812366, LR812365 | Netherlands | [23] | ||
64 | 4 | MT230033 | Italy | |
LR812305, LR812304, LR812303 | Portugal | [23] | ||
65 | 4 | LR812325, LR812324, LR812323, LR812326 | Denmark | [23] |
66 | 3 | LR812315, LR812314, LR812313 | Czechia | [23] |
67 | 1 | LR812382 | Great Britain | [23] |
68 | 1 | LR812357 | Slovenia | [23] |
69 | 5 | LR812331, LR812330, LR812329, LR812328, LR812327 | Denmark | [23] |
70 | 8 | MT232061, LR812292, LR812291, LR812287, LR812286, LR812285, LR812284, LR812140 | Albania | [23] |
71 | 2 | KX984130, KX984129 | Romania | |
72 | 1 | FM208737 | France | [12] |
73 | 1 | LR812320 | Czechia | [23] |
74 | 1 | LR812316 | Czechia | [23] |
75 | 1 | LR812471 | Great Britain | [23] |
76 | 1 | LR812364 | Netherlands | [23] |
77 | 1 | LR812360 | Netherlands | [23] |
78 | 1 | LR812359 | Slovenia | [23] |
79 | 3 | LR812358, LR812355, LR812353 | Slovenia | [23] |
80 | 1 | LR812368 | Netherlands | [23] |
81 | 1 | LR812356 | Slovenia | [23] |
82 | 1 | LR812322 | Czechia | [23] |
83 | 1 | LR812338 | Belgium | [23] |
84 | 1 | LR812308 | Portugal | [23] |
85 | 1 | D7 (OL547437) | Poland | |
86 | 1 | C6 (OL547438) | Poland | |
87 | 1 | B2 (OL547439) | Poland | |
88 | 1 | B10 (OL547440) | Poland | |
89 | 1 | LR812306 | Portugal | [23] |
90 | 2 | LR812302, LR812297 | France | [23] |
91 | 1 | LR812295 | Croatia | [23] |
92 | 1 | LR812354 | Slovenia | [23] |
93 | 1 | LR812461 | Italy | [23] |
Sampling Site (Farm) | Haplotype Number | ||||
---|---|---|---|---|---|
46 | 85 | 86 | 87 | 88 | |
A | 100% | - | - | - | - |
B | 80% | - | - | 10% | 10% |
C | 83% | - | 17% | - | - |
D | 90% | 10% | - | - | - |
E | 100% | - | - | - | - |
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Koziatek-Sadłowska, S.; Sokół, R. Genetic Characterization of the Poultry Red Mite (Dermanyssus gallinae) in Poland and a Comparison with European and Asian Isolates. Pathogens 2022, 11, 1301. https://doi.org/10.3390/pathogens11111301
Koziatek-Sadłowska S, Sokół R. Genetic Characterization of the Poultry Red Mite (Dermanyssus gallinae) in Poland and a Comparison with European and Asian Isolates. Pathogens. 2022; 11(11):1301. https://doi.org/10.3390/pathogens11111301
Chicago/Turabian StyleKoziatek-Sadłowska, Sylwia, and Rajmund Sokół. 2022. "Genetic Characterization of the Poultry Red Mite (Dermanyssus gallinae) in Poland and a Comparison with European and Asian Isolates" Pathogens 11, no. 11: 1301. https://doi.org/10.3390/pathogens11111301