An Acyl Carrier Protein Gene Affects Fatty Acid Synthesis and Growth of Hermetia illucens
Round 1
Reviewer 1 Report
The paper entitled " An acyl carrier protein gene affects fatty acid synthesis and growth of Hermetia illucens" . this paper aimed to To study the impact of FAS on the fatty acid metabolism of H. illucens, the authors focused on the functions of ACP, a key cofactor serving as the intermediate carrier during fatty acid biosynthesis. We developed an RNAi system to investigate the influence of downregulating the ACP gene in 3rd instar H. illucens larvae. The authors also used gas chromatography to assess changes in fatty acid composition in ACP-inhibited individuals. By determining the effect of the downregulation on larval, pupal, and adult biomass, survival rate, and development duration, we could further estimate the function of HiACP on the survival and growth of H. illucens and explore the possibility to enhance the fatty acid quality of this economic insect species
This field is the most fascinating application nowadays. the authors used adequate methodology and emphasized the results So, I recommend accepting this paper with minor revision
My comments:
1. the English language of this paper should be proofread
2. the relevant 2023 references should be taken
3. the reference that indicates the presence of the 6th instar should be mentioned
4. I recommend referring to the significant effect in the statistical analysis using the letters to facilitate the comparison
for example Fig. 6A 3rd will take a, 4th columns will take b and so on
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
In the current manuscript, the authors have investigated “An acyl carrier protein gene affects fatty acid synthesis and growth of Hermetia illucens”. The subjects full into the scope of the journal. The introduction is well managed with updated background, the materials and methods are comprehensive, results are precise but acceptable and discussion is well balance. I recommended, the paper is acceptable for publication. But used the update References
1. Nutritional composition of various insects and potential uses as alternative protein sources in animal diets.
2. Gryllus testaceus walker (crickets) farming management, chemical composition, nutritive profile, and their effect on animal digestibility
Author Response
Response to Reviewer 1 Comments
Point 1:
In the current manuscript, the authors have investigated “An acyl carrier protein gene affects fatty acid synthesis and growth of Hermetia illucens”. The subjects full into the scope of the journal. The introduction is well managed with updated background, the materials and methods are comprehensive, results are precise but acceptable and discussion is well balance. I recommended, the paper is acceptable for publication. But used the update References
Nutritional composition of various insects and potential uses as alternative protein sources in animal diets.
Gryllus testaceus walker (crickets) farming management, chemical composition, nutritive profile, and their effect on animal digestibility
Response 1:
Many thanks for reviewers’ suggestions to our manuscript. We have input two references that are recommended by reviewer, which can be found as references 26 and 27. We also added references published in 2023 to make our introduction and discussion more informative.
