MLN4924 Promotes Self-Renewal of Limbal Stem Cells and Ocular Surface Restoration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Feeder Cells
2.2. Isolation and Culture of LSCs
2.3. Crystal Violet Staining
2.4. Immunofluorescence Staining
2.5. RNA Extraction and Polymerase Chain Reaction (PCR)
2.6. Western Blotting
2.7. Corneal Epithelial Scratch Model
2.8. Statistical Analysis
3. Results
3.1. MLN4924 Promotes Clone Formation Rate and Clone Area of LSCs
3.2. MLN4924 Improves the Proliferative Capacity of LSCs In Vitro
3.3. MLN4924 Increases the Expression Levels of Ki67 and p63 in LSCs
3.4. MLN4924 Enhances the Preservation of Stemness of LSCs
3.5. MLN4924 Promotes Corneal Wound Healing
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, Q.; Shen, Y.; Wu, S.; Wei, H.; Zou, J.; Xu, S.; Ling, Q.; Kang, M.; Huang, H.; Chen, X.; et al. MLN4924 Promotes Self-Renewal of Limbal Stem Cells and Ocular Surface Restoration. J. Pers. Med. 2023, 13, 379. https://doi.org/10.3390/jpm13030379
Li Q, Shen Y, Wu S, Wei H, Zou J, Xu S, Ling Q, Kang M, Huang H, Chen X, et al. MLN4924 Promotes Self-Renewal of Limbal Stem Cells and Ocular Surface Restoration. Journal of Personalized Medicine. 2023; 13(3):379. https://doi.org/10.3390/jpm13030379
Chicago/Turabian StyleLi, Qingjian, Yankun Shen, Shinan Wu, Hong Wei, Jie Zou, Sanhua Xu, Qian Ling, Min Kang, Hui Huang, Xu Chen, and et al. 2023. "MLN4924 Promotes Self-Renewal of Limbal Stem Cells and Ocular Surface Restoration" Journal of Personalized Medicine 13, no. 3: 379. https://doi.org/10.3390/jpm13030379