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Volume 13
Issue 2
10.3390/jpm13020301
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Article
Peer-Review Record

Salivary-Based Cell-Free Mitochondrial DNA Level Is an Independent Prognostic Biomarker for Patients with Head and Neck Squamous Cell Carcinoma

J. Pers. Med. 2023, 13(2), 301; https://doi.org/10.3390/jpm13020301
by Lana Sayal 1, Omar Hamadah 1, Aroub AlMasri 2, Majdy Idrees 3, Issam Kassem 4, Wafa Habbal 5, Buthainah Alsalamah 6 and Omar Kujan 3,*
Reviewer 1: Anonymous
Reviewer 2:
Jan Mejzlik
Reviewer 3: Anonymous
J. Pers. Med. 2023, 13(2), 301; https://doi.org/10.3390/jpm13020301
Submission received: 20 January 2023 / Accepted: 7 February 2023 / Published: 8 February 2023
(This article belongs to the Special Issue Recent Advances in Oral Diseases)

Round 1

Reviewer 1 Report

In this study, the authors aimed to assess the utility of saliva-based cf-nDNA and cf-mtDNA in predicting the overall survival of patients with HNSCC. Saliva-based liquid biopsy was collected from 94 patients in total. They found that the absolute copy numbers of cf-nDNA and cf-mtDNA were statistically significantly higher among deceased patients than censored ones, p <0.05. Individuals with elevated levels of cf-nDNA or cf-mtDNA were associated with significantly poorer overall survival, p = <0.05. Univariate analysis showed that only the absolute copy number of cf-mtDNA is the sole predictor of overall survival. Whereas multivariate analysis showed that all the absolute copy numbers of cf-nDNA, the absolute copy numbers of cf-mtDNA, and the stage of HNSCC are predictors of overall survival. These data confirms that saliva is a reliable and non-invasive source of data to predict the overall survival of patients with HNSCC, where cf-mtDNA levels act as the sole predictor.

 

This study is novel and clinical meaningful, which would help to predict the overall survival of patients. The analysis is designed well and the data is presented clearly. 

 

This manuscript is very good.

Reviewer 2 Report

Mitochondrial DNA  studies represent new insight on the role of extranuclear DNA in oncogenesis. The scientific teams try to find how to use the knowledge in real patient treatment. The use of cell free mitochondrial DNA as the prognostic factor is novelistic.  The methods are clearly described. The statistics and graphs are clearly postulated. The results are adequately discussed in the end of the article. In my opinion this article does not require any changes. I recommend accept for publication in present form.

Reviewer 3 Report

Dear authors,
I have read your manuscript with great interest, and it appears suitable, as it stands, for consideration for publication.
Congratulations on the study and detailed methodological design.

Best regards.

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