Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Ce6
2.2. Cell Culture
2.3. Cell Viability Assay
2.4. Western Blot
2.5. Mouse Model
2.6. Xenograft Mouse Model Using B16F10 Melanoma Cell Line
2.7. Xenograft Mouse Model Using Panc02 Pancreatic Cell Line
2.8. PDT in Animal Model
2.9. Veterinary Case Study
2.10. PDD and PDT of Veterinary Case Study
3. Results
3.1. Absorbance and Fluorescence of Ce6
3.2. In Vitro Cytotoxicity of Ce6-PDT in Pancreatic Cancer
3.3. Effects of Ce6-PDT on Cell Apoptosis of AsPC-1 and MIA PaCa-2 Cells
3.4. In Vivo Effect of Ce6-PDT in Xenograft Mouse Model
3.5. Xenograft Mouse Model Using PANC02 Pancreatic Cell
3.6. Photodynamic Diagnosis in Dogs
3.7. Photodynamic Therapy in Dogs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | Species | Breed | Age (y) | Sex | Weight (kg) | Tumor Type | Tumor Site When PDT Application | Simultaneous Treatment |
---|---|---|---|---|---|---|---|---|
1 | Dog | Shih Tzu | 11 | F | 5 | Canine mammary carcinoma | Rt. Mammary gland | - |
2 | Dog | Maltese | 14 | M | 4 | Transitional cell carcinoma | Urinary bladder | Chemotherapy (Mitoxantrone) |
3 | Dog | Bichon Frise | 12 | F | 5.5 | Inflammatory mammary carcinoma | Mammary gland | Chemotherapy (Cyclophosphamide, Mitoxantrone) |
4 | Dog | Mixed | 11 | M | 5.5 | Malignant melanoma | Rt. Mandible, oral mucosa | Surgery (Rt. total hemimandibulectomy, Rt. submandibular lymphadenectomy), Chemotherapy (Carboplatin, Imatinib) |
5 | Dog | Dachshund | 5 | M | 9.3 | Histiocytic sarcoma | Axillary skin lesions, abdominal and groin skin, peripheral lymph nodes | Surgery, Chemotherapy (Doxorubicin, CCNU, Imatinib) |
Case No. | Light | No. of Treatment | Outcome | |
---|---|---|---|---|
Equipment | Superficial | Interstitial | ||
1 | Laser diode | 5 | 5 | Alive |
2 | Laser diode | 3 | 0 | Died by Metastasis |
3 | Laser diode | 7 | 5 | Died by Metastasis |
4 | Laser diode | 6 | 1 | Died by Metastasis |
5 | Laser diode | 5 | 7 | Alive |
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Shrestha, R.; Lee, H.J.; Lim, J.; Gurung, P.; Thapa Magar, T.B.; Kim, Y.-T.; Lee, K.; Bae, S.; Kim, Y.-W. Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors. Life 2022, 12, 2102. https://doi.org/10.3390/life12122102
Shrestha R, Lee HJ, Lim J, Gurung P, Thapa Magar TB, Kim Y-T, Lee K, Bae S, Kim Y-W. Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors. Life. 2022; 12(12):2102. https://doi.org/10.3390/life12122102
Chicago/Turabian StyleShrestha, Rajeev, Hyun Ji Lee, Junmo Lim, Pallavi Gurung, Til Bahadur Thapa Magar, Young-Tak Kim, Kija Lee, Seulgi Bae, and Yong-Wan Kim. 2022. "Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors" Life 12, no. 12: 2102. https://doi.org/10.3390/life12122102