Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Studies
2.2. EchoMRI Measurement
2.3. Western Blotting
2.4. Immunohistochemical Analysis and Quantification
2.5. Cell Isolation, Analysis, and Culture
2.6. Adipogenesis-Regulated Gene Expression PCR Array
2.7. Statistical Analysis
3. Results
3.1. Absence of the Tks4 Protein Resulted in Altered Adipose Tissue Composition
3.1.1. Phenotypic Description of the Three Adipose Depots in Tks4-KO Mice
3.1.2. Tks4-KO and WT Stromal Vascular Fraction (SVF) Cells Shared a Similar De Novo Adipogenic Potential
3.1.3. UCP1 Expression Was Higher in Tks4-KO WAT Than in WT WAT
3.2. Loss of Tks4 Resulted in an Altered Gene Expression Pattern in White Adipocytes
3.2.1. Results of the Adipogenesis-Regulated Gene Expression PCR Array
3.2.2. The Differentially Expressed Adipogenesis-Related Genes Formed A Network with PPARγ as Its Central Regulator
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Vas, V.; Háhner, T.; Kudlik, G.; Ernszt, D.; Kvell, K.; Kuti, D.; Kovács, K.J.; Tóvári, J.; Trexler, M.; Merő, B.L.; et al. Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing. Cells 2019, 8, 831. https://doi.org/10.3390/cells8080831
Vas V, Háhner T, Kudlik G, Ernszt D, Kvell K, Kuti D, Kovács KJ, Tóvári J, Trexler M, Merő BL, et al. Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing. Cells. 2019; 8(8):831. https://doi.org/10.3390/cells8080831
Chicago/Turabian StyleVas, Virag, Tamás Háhner, Gyöngyi Kudlik, Dávid Ernszt, Krisztián Kvell, Dániel Kuti, Krisztina J. Kovács, József Tóvári, Mária Trexler, Balázs L. Merő, and et al. 2019. "Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing" Cells 8, no. 8: 831. https://doi.org/10.3390/cells8080831