Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. The Microfluidic Chip Design
2.3. The Microfluidic Chip Fabrication
2.4. Cell Culture
2.5. The Detection of Metabolite Characteristics
2.6. Statistical Analysis
3. Results
3.1. Actual Product of the Fabricated PEGDA Hydrogel Microfluidic Chip
3.2. Cell Morphological Observation
3.3. The Metabolite Characteristics
3.4. HCA, PCA, and LDA Analyses
4. Discussion
4.1. Performance Characteristic of the PEGDA Microfluidic Chip
4.2. Effect of Cell Culture on the PEGDA Microfluidic Chip
4.3. Validation of Detection Application of the PEGDA Microfluidic Chip
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
3D | Three-dimensional |
HCA | Hierarchical cluster analysis |
HEMA | 2-hydroxyethyl methacrylate |
IARC | International Agency for Research on Cancer |
LDA | Linear discriminant analysis |
NVP | 1-vinyl-2-pyrrolidone |
PBS | Phosphate-buffered saline |
PCA | Principal component analysis |
PDMS | Poly-dimethylsiloxane |
PEG | Polyethylene glycol |
PEGDA | Poly(ethylene glycol) diacrylate |
PVDF | Polyvinylidene fluoride |
RGB | Red, green, and blue |
SED | Squared Euclidean distance |
References
- Alamri, F.B.; Sobahi, T.R.; Althagbi, H.I.; Abdel-Lateff, A.; Alfaifi, M.Y.; Mohammed, A.Y.; Abdel-Latif, E.; Alarif, W.M. Bioactivity and molecular docking of lactones isolated from Centaurea pseudosinaica Czerep. Saudi Pharm. J. 2023, 31, 773–782. [Google Scholar] [CrossRef]
- Guida, F.; Kidman, R.; Ferlay, J.; Schüz, J.; Soerjomataram, I.; Kithaka, B.; Ginsburg, O.; Mailhot Vega, R.B.; Galukande, M.; Parham, G.; et al. Global and regional estimates of orphans attributed to maternal cancer mortality in 2020. Nat. Med. 2022, 28, 2563–2572. [Google Scholar] [CrossRef] [PubMed]
- Gadaleta, E.A.-O.; Thorn, G.A.-O.; Ross-Adams, H.A.-O.X.; Jones, L.J.; Chelala, C.A.-O. Field cancerization in breast cancer. J. Pathol. 2022, 257, 561–574. [Google Scholar] [CrossRef]
- Hanna, K.; Krzoska, E.; Shaaban, A.M.; Muirhead, D.; Abu-Eid, R.; Speirs, V. Raman spectroscopy: Current applications in breast cancer diagnosis, challenges and future prospects. Br. J. Cancer 2022, 126, 1125–1139. [Google Scholar] [CrossRef] [PubMed]
- Yafia, M.; Ymbern, O.; Olanrewaju, A.O.; Parandakh, A.; Sohrabi Kashani, A.; Renault, J.; Jin, Z.; Kim, G.; Ng, A.; Juncker, D. Microfluidic chain reaction of structurally programmed capillary flow events. Nature 2022, 605, 464–469. [Google Scholar] [CrossRef]
- Gökçe, O.; Castonguay, S.; Temiz, Y.; Gervais, T.; Delamarche, E. Self-coalescing flows in microfluidics for pulse-shaped delivery of reagents. Nature 2019, 574, 228–232. [Google Scholar] [CrossRef]
- Ahn, J.; Ohk, K.; Won, J.; Choi, D.-H.; Jung, Y.H.; Yang, J.H.; Jun, Y.; Kim, J.-A.; Chung, S.; Lee, S.-H. Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system. Nat. Commun. 2023, 14, 1488. [Google Scholar] [CrossRef]
- Paiè, P.; Martínez Vázquez, R.; Osellame, R.; Bragheri, F.; Bassi, A. Microfluidic Based Optical Microscopes on Chip. Cytometry A 2018, 93, 987–996. [Google Scholar] [CrossRef]
- Macková, H.; Hlídková, H.; Kaberova, Z.; Proks, V.; Kučka, J.; Patsula, V.; Vetrik, M.; Janoušková, O.; Podhorská, B.; Pop-Georgievski, O.; et al. Thiolated poly(2-hydroxyethyl methacrylate) hydrogels as a degradable biocompatible scaffold for tissue engineering. Mater. Sci. Eng. C Mater. Biol. Appl. 2021, 131, 112500. [Google Scholar] [CrossRef]
- Ravi, S.; Chokkakula, L.P.P.; Giri, P.S.; Korra, G.; Dey, S.R.; Rath, S.N. 3D Bioprintable Hypoxia-Mimicking PEG-Based Nano Bioink for Cartilage Tissue Engineering. ACS Appl. Mater. Interfaces 2023, 16, 19921–19936. [Google Scholar] [CrossRef]
- Wang, Z.; Ye, Q.; Yu, S.; Akhavan, B. Poly Ethylene Glycol (PEG)-based Hydrogels for Drug Delivery in Cancer Therapy. Adv. Healthc. Mater. 2023, 18, 2300105. [Google Scholar] [CrossRef] [PubMed]
- Ju, H.J.; Ji, Y.B.; Kim, S.; Yun, H.W.; Kim, J.H.; Min, B.H.; Kim, M.S. Development and In Vivo Assessment of an Injectable Cross-Linked Cartilage Acellular Matrix-PEG Hydrogel Scaffold Derived from Porcine Cartilage for Tissue Engineering. Macromol. Biosci. 2023, 6, 2300029. [Google Scholar] [CrossRef] [PubMed]
- Hou, P.; Zhang, N.; Wu, R.; Xu, W.; Hou, Z. Photo-cross-linked biodegradable hydrogels based on n-arm-poly(ethylene glycol), poly(ε-caprolactone) and/or methacrylic acid for controlled drug release. J. Biomater. Appl. 2017, 32, 511–523. [Google Scholar] [CrossRef] [PubMed]
- Ahmed, E.M. Hydrogel: Preparation, characterization, and applications: A review. J. Adv. Res. 2015, 6, 105–121. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sharifi, F.; Patel, B.B.; McNamara, M.C.; Meis, P.J.; Roghair, M.N.; Lu, M.; Montazami, R.; Sakaguchi, D.S.; Hashemi, N.N. Photo-Cross-Linked Poly(ethylene glycol) Diacrylate Hydrogels: Spherical Microparticles to Bow Tie-Shaped Microfibers. ACS Appl. Mater. Interfaces 2019, 11, 18797–18807. [Google Scholar] [CrossRef]
- Li, Z.; Li, C.; Sun, W.; Bai, Y.; Li, Z.; Deng, Y. A Controlled Biodegradable Triboelectric Nanogenerator Based on PEGDA/Laponite Hydrogels. ACS Appl. Mater. Interfaces 2023, 15, 12787–12796. [Google Scholar] [CrossRef]
- Khurana, B.; Gierlich, P.; Meindl, A.; Gomes-da-Silva, L.C.; Senge, M.O. Hydrogels: Soft matters in photomedicine. Photochem. Photobiol. Sci. 2019, 18, 2613–2656. [Google Scholar] [CrossRef]
- Clasky, A.J.; Watchorn, J.D.; Chen, P.Z.; Gu, F.X. From prevention to diagnosis and treatment: Biomedical applications of metal nanoparticle-hydrogel composites. Acta Biomater. 2021, 122, 1–25. [Google Scholar] [CrossRef]
- Razzaghi, M.; Seyfoori, A.; Pagan, E.; Askari, E.; Hassani Najafabadi, A.; Akbari, M. 3D Printed Hydrogel Microneedle Arrays for Interstitial Fluid Biomarker Extraction and Colorimetric Detection. Polymers 2023, 15, 1389. [Google Scholar] [CrossRef]
- Wang, Z.; Jiang, H.; Wu, G.; Li, Y.; Zhang, T.; Zhang, Y.; Wang, X. Shape-Programmable Three-Dimensional Microfluidic Structures. ACS Appl. Mater. Interfaces 2022, 14, 15599–15607. [Google Scholar] [CrossRef]
- AbuMadighem, A.; Shuchat, S.; Lunenfeld, E.; Yossifon, G.; Huleihel, M. Testis on a chip-a microfluidic three-dimensional culture system for the development of spermatogenesisin-vitro. Biofabrication 2022, 14, 5090. [Google Scholar] [CrossRef] [PubMed]
- Lim, K.S.; Galarraga, J.H.; Cui, X.; Lindberg, G.C.J.; Burdick, J.A.; Woodfield, T.B.F. Fundamentals and Applications of Photo-Cross-Linking in Bioprinting. Chem. Rev. 2020, 120, 10662–10694. [Google Scholar] [CrossRef] [PubMed]
- Darmawan, B.A.; Lee, S.B.; Nan, M.; Nguyen, V.D.; Park, J.-O.; Choi, E. Shape-Tunable UV-Printed Solid Drugs for Personalized Medicine. Polymers 2022, 14, 2714. [Google Scholar] [CrossRef] [PubMed]
- Xu, Z.; Liu, G.; Huang, J.; Wu, J. Novel Glucose-Responsive Antioxidant Hybrid Hydrogel for Enhanced Diabetic Wound Repair. ACS Appl. Mater. Interfaces 2022, 14, 7680–7689. [Google Scholar] [CrossRef]
- Posritong, S.; Flores Chavez, R.; Chu, T.G.; Bruzzaniti, A. A Pyk2 inhibitor incorporated into a PEGDA-gelatin hydrogel promotes osteoblast activity and mineral deposition. Biomed. Mater. 2019, 14, 025015. [Google Scholar] [CrossRef]
- Huang, L.; Li, W.; Guo, M.; Huang, Z.; Chen, Y.; Dong, X.; Li, Y.; Zhu, L. Silver doped-silica nanoparticles reinforced poly (ethylene glycol) diacrylate/hyaluronic acid hydrogel dressings for synergistically accelerating bacterial-infected wound healing. Carbohydr. Polym. 2023, 304, 120450. [Google Scholar] [CrossRef]
- Simič, R.; Mandal, J.; Zhang, K.; Spencer, N.D. Oxygen inhibition of free-radical polymerization is the dominant mechanism behind the “mold effect” on hydrogels. Soft Matter 2021, 17, 6394–6403. [Google Scholar] [CrossRef]
- Xie, M.; Su, J.; Zhou, S.; Li, J.; Zhang, K. Application of Hydrogels as Three-Dimensional Bioprinting Ink for Tissue Engineering. Gels 2023, 9, 88. [Google Scholar] [CrossRef]
- Card, M.; Alejandro, R.; Roxbury, D. Decoupling Individual Optical Nanosensor Responses Using a Spin-Coated Hydrogel Platform. ACS Appl. Mater. Interfaces 2023, 15, 1772–1783. [Google Scholar] [CrossRef]
- Schesny, M.K.; Monaghan, M.; Bindermann, A.H.; Freund, D.; Seifert, M.; Eble, J.A.; Vogel, S.; Gawaz, M.P.; Hinderer, S.; Schenke-Layland, K. Preserved bioactivity and tunable release of a SDF1-GPVI bi-specific protein using photo-crosslinked PEGda hydrogels. Biomaterials 2014, 35, 7180–7187. [Google Scholar] [CrossRef]
- Testore, D.; Zoso, A.; Kortaberria, G.; Sangermano, M.; Chiono, V. Electroconductive Photo-Curable PEGDA-Gelatin/PEDOT:PSS Hydrogels for Prospective Cardiac Tissue Engineering Application. Front. Bioeng. Biotechnol. 2022, 10, 897575. [Google Scholar] [CrossRef]
- Babić Radić, M.M.; Filipović, V.V.; Vuković, J.S.; Vukomanović, M.; Ilic-Tomic, T.; Nikodinovic-Runic, J.; Tomić, S.L. 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO(2) as a Promising Curcumin Release Platform. Polymers 2023, 15, 1643. [Google Scholar] [CrossRef] [PubMed]
- Rimmer, S.; Spencer, P.; Nocita, D.; Sweeney, J.; Harrison, M.; Swift, T. Chain-Extendable Crosslinked Hydrogels Using Branching RAFT Modification. Gels 2023, 9, 235. [Google Scholar] [CrossRef] [PubMed]
- Yun, T.; Cheng, P.; Qian, F.; Cheng, Y.; Lu, J.; Lv, Y.; Wang, H. Balancing the decomposable behavior and wet tensile mechanical property of cellulose-based wet wipe substrates by the aqueous adhesive. Int. J. Biol. Macromol. 2020, 164, 1898–1907. [Google Scholar] [CrossRef] [PubMed]
- Zhai, G.; Wu, J.; Yuan, Z.; Li, H.; Sun, D. Robust Superhydrophobic PDMS@SiO(2)@UiO66-OSiR Sponge for Efficient Water-in-Oil Emulsion Separation. Inorg. Chem. 2023, 62, 5447–5457. [Google Scholar] [CrossRef]
- Wang, Y.; Huo, D.; Wu, H.; Li, J.; Zhang, Q.; Deng, B.; Zhou, J.; Yang, M.; Hou, C. A visual sensor array based on an indicator displacement assay for the detection of carboxylic acids. Mikrochim. Acta 2019, 186, 496. [Google Scholar] [CrossRef]
- Zou, C.; Liu, Z.; Wang, X.; Liu, H.; Yang, M.; Huo, D.; Hou, C. A paper-based visualization chip based on nitrogen-doped carbon quantum dots nanoprobe for Hg(Ⅱ) detection. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2022, 265, 120346. [Google Scholar] [CrossRef]
- Zhang, Q.; Li, J.; Wang, Y.; Ma, Y.; He, M.; Zhao, D.; Huo, D.; Lu, L.; Hou, C. Detection of aldehydes by gold nanoparticle colorimetric array based on Tollens’ reagent. Anal. Methods 2021, 13, 5478–5486. [Google Scholar] [CrossRef]
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Guo, M.; Deng, Y.; Huang, J.; Huang, Y.; Deng, J.; Wu, H. Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells. Polymers 2023, 15, 3183. https://doi.org/10.3390/polym15153183
Guo M, Deng Y, Huang J, Huang Y, Deng J, Wu H. Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells. Polymers. 2023; 15(15):3183. https://doi.org/10.3390/polym15153183
Chicago/Turabian StyleGuo, Mingyi, Yan Deng, Junqiu Huang, Yanping Huang, Jing Deng, and Huachang Wu. 2023. "Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells" Polymers 15, no. 15: 3183. https://doi.org/10.3390/polym15153183