An Investigation for Skin Tissue Regeneration Enhancement/Augmentation by Curcumin-Loaded Self-Emulsifying Drug Delivery System (SEDDS)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation and Optimization of Curcumin-Loaded SEDDS
2.3. Droplet Size, Zeta Potential, and PDI
2.4. Drug Content (DC) and Entrapment Efficiency (EE)
2.5. In Vitro Drug Release
2.6. Stability of the Nano-Emulsion Formed by SEDDS
2.7. Thermodynamic Stability of SEDDS
2.7.1. Centrifugation
2.7.2. Heating/Cooling Cycle
2.7.3. Freeze–Thaw Cycle
2.8. Long-Term Storage Stability
2.9. Cytotoxicity/Cell Viability
2.10. Cell Migration/Scratch Assay
2.11. Intracellular ROS Measurement/Antioxidant Assay
2.12. Cellular Uptake
2.13. Diabetic Wound Healing
2.14. Statistical Analysis
3. Results and Discussion
3.1. Formulation Optimization
3.2. Droplet Size, Zeta Potential, and PDI
3.3. Drug Content and Entrapment Efficiency
3.4. In Vitro Drug Release
3.5. Stability of the SEDDS and Their Nano-Emulsions
3.6. Cytotoxicity/Cell Viability
3.7. Cell Migration Analysis
3.8. Intracellular ROS Measurement/Antioxidant Assay
3.9. Cellular Uptake
3.10. Diabetic Wound Healing
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Formulations | Drug (mg) | Tween 80 (mg) | PEG 600 (mg) | Ethyl Oleate (mg) | Water (mg) | Observation | Grade | Emulsification Time (Min) |
---|---|---|---|---|---|---|---|---|
F1 | 4 | 83 | 143 | 120 | 650 | Stable with no sedimentation/creaming | A | <1 |
F2 | 4 | 87 | 148 | 111 | 650 | Stable with no sedimentation/creaming | A | <1 |
F3 | 4 | 90 | 156 | 100 | 650 | Stable with no sedimentation/creaming | A | <1 |
F4 | 4 | 100 | 160 | 136 | 600 | Creaming at the surface. No drug settling | B | 2 |
F5 | 4 | 116 | 160 | 120 | 600 | Creaming at the surface. No drug settling | B | 2 |
F6 | 4 | 126 | 160 | 110 | 600 | Creaming at the surface. No drug settling | B | 2 |
F7 | 4 | 136 | 160 | 100 | 600 | Creaming at the surface. No drug settling | B | 2 |
F8 | 4 | 105 | 166 | 135 | 590 | Creaming at the surface. No drug settling | B | 2 |
F9 | 4 | 108 | 169 | 139 | 580 | Creaming at the surface. No drug settling | B | 2 |
F10 | 4 | 111 | 172 | 143 | 570 | Creaming at the surface. No drug settling | B | 2 |
F11 | 4 | 114 | 175 | 147 | 560 | Creaming at the surface. Little Drug settling | C | 3 |
F12 | 4 | 117 | 178 | 151 | 550 | Creaming at the surface. No drug settling | B | 2 |
F13 | 4 | 120 | 181 | 155 | 540 | Creaming at the surface. No drug settling | B | 2 |
F14 | 4 | 123 | 184 | 159 | 530 | Creaming at the surface. No drug settling | B | 2 |
F15 | 4 | 126 | 187 | 163 | 520 | Creaming at the surface. No drug settling | B | 2 |
F16 | 4 | 129 | 190 | 167 | 510 | Creaming at the surface. No drug settling | B | 2 |
F17 | 4 | 132 | 193 | 171 | 500 | Creaming at the surface. No drug settling | B | 2 |
F18 | 4 | 135 | 196 | 175 | 490 | Creaming at the surface. No drug settling | B | 2 |
F19 | 4 | 138 | 199 | 179 | 480 | Creaming at the surface. No drug settling | B | 2 |
F20 | 4 | 72 | 133 | 91 | 700 | Creaming at the surface. Little drug settling | C | 3 |
F21 | 4 | 57 | 118 | 71 | 750 | Creaming at the surface. Little drug settling | C | 3 |
F22 | 4 | 42 | 103 | 51 | 800 | Creaming at the surface. Little drug settling | C | 3 |
Formulations | Grade | Thermodynamic Stability Tests | |||
---|---|---|---|---|---|
Centrifugation | Heating and Cooling Cycle | Freeze–Thaw Cycle | Robustness to Dilution | ||
F1 | A | Pass | Pass | Pass | Pass |
F2 | A | Pass | Pass | Pass | Pass |
F3 | A | Pass | Pass | Pass | Pass |
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Mahmood, S.; Bhattarai, P.; Khan, N.R.; Subhan, Z.; Razaque, G.; Albarqi, H.A.; Alqahtani, A.A.; Alasiri, A.; Zhu, L. An Investigation for Skin Tissue Regeneration Enhancement/Augmentation by Curcumin-Loaded Self-Emulsifying Drug Delivery System (SEDDS). Polymers 2022, 14, 2904. https://doi.org/10.3390/polym14142904
Mahmood S, Bhattarai P, Khan NR, Subhan Z, Razaque G, Albarqi HA, Alqahtani AA, Alasiri A, Zhu L. An Investigation for Skin Tissue Regeneration Enhancement/Augmentation by Curcumin-Loaded Self-Emulsifying Drug Delivery System (SEDDS). Polymers. 2022; 14(14):2904. https://doi.org/10.3390/polym14142904
Chicago/Turabian StyleMahmood, Saima, Prapanna Bhattarai, Nauman Rahim Khan, Zakia Subhan, Ghulam Razaque, Hassan A. Albarqi, Abdulsalam A. Alqahtani, Ali Alasiri, and Lin Zhu. 2022. "An Investigation for Skin Tissue Regeneration Enhancement/Augmentation by Curcumin-Loaded Self-Emulsifying Drug Delivery System (SEDDS)" Polymers 14, no. 14: 2904. https://doi.org/10.3390/polym14142904