Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganism and Inoculum Preparation
2.2. Preparation of Nutrient Media
2.3. Fermentation
2.4. Purification of BC
2.5. Calculation of BC Yield
2.6. Analytical Techniques
2.7. Prosthetic Tension-Free Hernioplasty
3. Results and Discussion
3.1. The Number of BC Pellicle Removals
3.2. Glucose Concentration and BC Yield
3.3. BC Properties
3.4. Outcomes of Tension-Free Hernioplasty
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Option | 1 | 2 | 3 |
---|---|---|---|
Vessel material | Food-grade polypropylene | Enamelled iron | Glass |
Vessel shape | Rectangular parallelepiped | Cylinder | Tapered sphere |
Geometric dimensions, cm | Length 9.0 Width 6.4 Height 4.8 | Radius 20.5 Height 35 | Radius 17.4 Neck radius 10.4 |
Ratio of growth medium area to vessel neck area | 1:1 | 1:1 | 2.8:1 |
Growth medium surface area S, cm2 | 65 | 1320 | 946 |
Scale-up ratio by area | - | 1:20 | 1:15 |
Vessel volume, L | 0.25 | 45.0 | 17.3 |
Growth medium volume, L | 0.2 | 8.0 | 8.0 |
Scale-up ratio by volume | - | 1:40 | 1:40 |
Growth medium layer height h, cm | 3.6 | 6.1 | from 0 to 12.5 |
Ratio of S/h, cm | 18 | 218 | from 0 to 76 |
Gel-Film Removals | I | II | III | IV | V | VI | VII | VIII | IX | X | XI |
---|---|---|---|---|---|---|---|---|---|---|---|
Biosynthesis in small vessel 1 | |||||||||||
Biosynthesis time for a given BC sample (days) | 3 | 3 | 3 | 5 | 8 | – | – | – | – | – | – |
Total biosynthesis time (days) | 3 | 6 | 9 | 14 | 22 | – | – | – | – | – | – |
Biosynthesis in cylinder-shaped vessel 2 | |||||||||||
Biosynthesis time for a given BC sample (days) | 3 | 3 | 3 | 3 | 4 | 4 | 5 | – | – | – | – |
Total biosynthesis time (days) | 3 | 6 | 9 | 12 | 16 | 20 | 25 | – | – | – | – |
Biosynthesis in tapered sphere-shaped vessel 3 | |||||||||||
Biosynthesis time for a given BC sample (days) | 3 | 3 | 4 | 4 | 5 | 5 | 6 | 6 | 6 | 7 | 11 |
Total biosynthesis time (days) | 3 | 6 | 10 | 14 | 19 | 24 | 30 | 36 | 42 | 49 | 60 |
Vessel | 1 | 2 | 3 |
---|---|---|---|
Surface area of growth medium S, cm2 | 65 | 1320 | 946 |
Number of gel-films | 5 | 7 | 11 |
Overall surface area of BC samples, cm2 | 325 | 9240 | 10,406 |
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Skiba, E.A.; Shavyrkina, N.A.; Budaeva, V.V.; Sitnikova, A.E.; Korchagina, A.A.; Bychin, N.V.; Gladysheva, E.K.; Pavlov, I.N.; Zharikov, A.N.; Lubyansky, V.G.; et al. Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles. Polymers 2021, 13, 2118. https://doi.org/10.3390/polym13132118
Skiba EA, Shavyrkina NA, Budaeva VV, Sitnikova AE, Korchagina AA, Bychin NV, Gladysheva EK, Pavlov IN, Zharikov AN, Lubyansky VG, et al. Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles. Polymers. 2021; 13(13):2118. https://doi.org/10.3390/polym13132118
Chicago/Turabian StyleSkiba, Ekaterina A., Nadezhda A. Shavyrkina, Vera V. Budaeva, Anastasia E. Sitnikova, Anna A. Korchagina, Nikolay V. Bychin, Evgenia K. Gladysheva, Igor N. Pavlov, Andrey N. Zharikov, Vladimir G. Lubyansky, and et al. 2021. "Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles" Polymers 13, no. 13: 2118. https://doi.org/10.3390/polym13132118