Cancer-Associated Fibroblasts Modulate Transcriptional Signatures Involved in Proliferation, Differentiation and Metastasis in Head and Neck Squamous Cell Carcinoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cells and Culture Conditions
2.2. Co-Culture of Tumor Cells and CAFs
2.3. Magnetic Activated Cell Sorting (MACS)
2.4. Microarray Analysis
2.5. qRT-PCR
2.6. Immunohistochemistry (IHC)
2.7. Tumor Material and Patient Characteristics
2.8. Statistical Analysis
3. Results
3.1. Microarray Analysis in 2D Cultures
3.2. Microarray Analysis in 3D Cultures
3.3. Verification of Microarray Results Using qRT-PCR
3.4. Verification of Microarray Results on Protein Level
3.5. Expression of CAF-Regulated Genes in Tumor Tissue and Their Impact on Radiotherapy Response
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene ID | Log2 Fold Change 2D | Gene ID | Log2 Fold Change 3D | Gene ID | Log2 Fold Change 2D | Gene ID | Log2 Fold Change 3D |
---|---|---|---|---|---|---|---|
POSTN | ↑ 12.5 | MMP1 | ↑ 152.95 | GBP4 | ↓ −3.12 | IVL | ↓ −20.64 |
GREM1 | ↑ 4.07 | MMP9 | ↑ 49.34 | KLK6 | ↓ −2.7 | CNFN | ↓ −18.34 |
COL1A1 | ↑ 2.46 | BGN | ↑ 41.26 | KLK7 | ↓ −2.65 | KRT1 | ↓ −16.61 |
COL1A2 | ↑ 2.39 | COL6A3 | ↑ 28 | SPRR2A | ↓ −2.6 | DSG1 | ↓ −13.48 |
COL6A3 | ↑ 2.17 | POSTN | ↑ 27.84 | FYB | ↓ −2.55 | KRT10 | ↓ −12.64 |
BGN | ↑ 2.08 | TNC | ↑ 17.97 | GABRP | ↓ −2.54 | MUC15 | ↓ −10.81 |
OR4N2 | ↑ 2 | COL1A2 | ↑ 12.94 | ADGRF4 | ↓ −2.51 | SPINK5 | ↓ −9.66 |
ID1 | ↑ 2 | IGFBP5 | ↑ 12.65 | IDO1 | ↓ −2.45 | RPTN | ↓ −9.35 |
FMOD | ↑ 11.36 | CLDN4 | ↓ −2.44 | CLCA4 | ↓ −8.91 | ||
PDGFRB | ↑ 10.98 | GBP6 | ↓ −2.44 | SDR9C7 | ↓ −8.53 | ||
COL1A1 | ↑ 10.91 | KRT15 | ↓ −2.36 | SPINK7 | ↓ −8.1 | ||
DCN | ↑ 10.18 | LIPH | ↓ −2.35 | LIPK | ↓ −7.95 | ||
HAS2 | ↑ 9.07 | CLDN8 | ↓ −2.34 | FOXN1 | ↓ −7.53 | ||
EDNRA | ↑ 8.77 | MMP7 | ↓ −2.33 | BNIPL | ↓ −6.93 | ||
THBS1 | ↑ 7.51 | SYTL2 | ↓ −2.32 | SCEL | ↓ −6.66 | ||
GREM1 | ↑ 6.67 | CRABP2 | ↓ −2.31 | SPRR1A | ↓ −6.52 | ||
AMTN | ↑ 6.39 | FRK | ↓ −2.3 | CES1 | ↓ −5.43 | ||
SPARC | ↑ 6.19 | TMPRSS4 | ↓ −2.27 | MIR203A | ↓ −5.06 | ||
EMP3 | ↑ 5.51 | CYP4F3 | ↓ −2.19 | OCLN | ↓ −4.45 | ||
CTSK | ↑ 3.84 | TLR6 | ↓ −2.16 | KLK10 | ↓ −4.15 |
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Wiechec, E.; Magan, M.; Matic, N.; Ansell-Schultz, A.; Kankainen, M.; Monni, O.; Johansson, A.-C.; Roberg, K. Cancer-Associated Fibroblasts Modulate Transcriptional Signatures Involved in Proliferation, Differentiation and Metastasis in Head and Neck Squamous Cell Carcinoma. Cancers 2021, 13, 3361. https://doi.org/10.3390/cancers13133361
Wiechec E, Magan M, Matic N, Ansell-Schultz A, Kankainen M, Monni O, Johansson A-C, Roberg K. Cancer-Associated Fibroblasts Modulate Transcriptional Signatures Involved in Proliferation, Differentiation and Metastasis in Head and Neck Squamous Cell Carcinoma. Cancers. 2021; 13(13):3361. https://doi.org/10.3390/cancers13133361
Chicago/Turabian StyleWiechec, Emilia, Mustafa Magan, Natasa Matic, Anna Ansell-Schultz, Matti Kankainen, Outi Monni, Ann-Charlotte Johansson, and Karin Roberg. 2021. "Cancer-Associated Fibroblasts Modulate Transcriptional Signatures Involved in Proliferation, Differentiation and Metastasis in Head and Neck Squamous Cell Carcinoma" Cancers 13, no. 13: 3361. https://doi.org/10.3390/cancers13133361