In Vitro Antiproliferative Apoptosis Induction and Cell Cycle Arrest Potential of Saudi Sidr Honey against Colorectal Cancer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection of Honey Sample
2.2. Phytochemical Analysis of Saudi Sidr Honey
2.3. Cell Culture
2.4. Cell Viability Assay
2.5. Colony Formation Assay
2.6. Annexin V–PI Apoptosis Assay
2.7. Cell Cycle Analysis
2.8. Molecular Docking Analysis
2.9. Statistical Analysis
3. Results
3.1. Phytochemical Composition of Saudi Sidr Honey
3.2. Anticancer Activity of Saudi Sidr Honey
3.3. Effect of Saudi Sidr Honey on Colony Formation of HCT-116 Cells
3.4. Apoptosis Induction by Saudi Sidr Honey
3.5. Saudi Sidr Honey Induces G1 Cell Cycle Arrest
3.6. Molecular Docking Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compounds | Structure | RT (min) |
---|---|---|
Glyceraldehyde | 6.526 | |
Butanedioic acid | 17.681 | |
Tetrahydro-4H-pyran-4-ol | 19.335 | |
1-Cyclohexylimidazolidin-2-one | 23.937 | |
3-butyl-3-methyl-1-Cyclohexanone | 25.170 | |
4-Butyl-3-methoxy-2-cyclopenten-1-one | 25.284 | |
2,2,3,3-Tetramethylcyclopropanecarboxylic acid | 29.099 | |
3,5-Dihydroxy-2-(3-methylbut-2-en-1-yl) | 29.709 |
Compounds | Mode of Analysis | Structure | RT (min) |
---|---|---|---|
Gerberinol | Positive | 1.014 | |
Osmaronin | Positive | 1.393 | |
Retronecine | Positive | 1.469 | |
N-(1-Deoxy-1-fructosyl) phenylalanine | Positive | 1.971 | |
C16 Sphinganine | Positive | 12.085 | |
6-Hydroxypentadecanedioic acid | Positive | 12.788 | |
Sphinganine | Positive | 13.77 | |
Oleamide | Positive | 20.347 | |
Hamamelose | Negative | 1.236 | |
2-O-b-D-Galactopyranosyl-D-xylose | Negative | 1.345 | |
2,5-Didehydro-D-gluconate | Negative | 1.423 | |
Sarmentosin epoxide | Negative | 1.446 | |
Hellicoside | Negative | 7.818 | |
Palmidin C | Negative | 17.62 | |
2-O-a-L-Fucopyranosyl-galactose | Negative | 21.505 |
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Qanash, H.; Bazaid, A.S.; Binsaleh, N.K.; Patel, M.; Althomali, O.W.; Sheeha, B.B. In Vitro Antiproliferative Apoptosis Induction and Cell Cycle Arrest Potential of Saudi Sidr Honey against Colorectal Cancer. Nutrients 2023, 15, 3448. https://doi.org/10.3390/nu15153448
Qanash H, Bazaid AS, Binsaleh NK, Patel M, Althomali OW, Sheeha BB. In Vitro Antiproliferative Apoptosis Induction and Cell Cycle Arrest Potential of Saudi Sidr Honey against Colorectal Cancer. Nutrients. 2023; 15(15):3448. https://doi.org/10.3390/nu15153448
Chicago/Turabian StyleQanash, Husam, Abdulrahman S. Bazaid, Naif K. Binsaleh, Mitesh Patel, Omar W. Althomali, and Bodor Bin Sheeha. 2023. "In Vitro Antiproliferative Apoptosis Induction and Cell Cycle Arrest Potential of Saudi Sidr Honey against Colorectal Cancer" Nutrients 15, no. 15: 3448. https://doi.org/10.3390/nu15153448