Mechanistic Action of Cell Cycle Arrest and Intrinsic Apoptosis via Inhibiting Akt/mTOR and Activation of p38-MAPK Signaling Pathways in Hep3B Liver Cancer Cells by Prunetrin—A Flavonoid with Therapeutic Potential
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Line and Maintenance
2.2. Cytotoxicity Test
2.3. Colony Formation Assay
2.4. DAPI Staining
2.5. Cell Cycle Analysis
2.6. Double Staining
2.7. Western Blotting
2.8. Statistical Analysis
3. Results
3.1. Prunetrin Induces Cytotoxicity in Hep3B Cells
3.2. Morphological Assessment of Hep3B Cells Treated with PUR
3.3. Prunetrin Induces Cell Cycle Arrest in Hep3B Cells
3.4. Prunetrin Induces Apoptosis and Increases the Expression of Apoptotic Hallmark Proteins
3.5. Prunetrin Activates an Intrinsic Apoptotic Pathway in Hep3B Cells
3.6. Prunetrin Does Not Induce Extrinsic Apoptosis and Other Cell Death Modes in Hep3B Cells
3.7. Prunetrin Inhibits AKT/mTOR Signaling Pathway
3.8. Effect of Prunetrin on MAPK Pathway
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Abusaliya, A.; Jeong, S.H.; Bhosale, P.B.; Kim, H.H.; Park, M.Y.; Kim, E.; Won, C.K.; Park, K.I.; Heo, J.D.; Kim, H.W.; et al. Mechanistic Action of Cell Cycle Arrest and Intrinsic Apoptosis via Inhibiting Akt/mTOR and Activation of p38-MAPK Signaling Pathways in Hep3B Liver Cancer Cells by Prunetrin—A Flavonoid with Therapeutic Potential. Nutrients 2023, 15, 3407. https://doi.org/10.3390/nu15153407
Abusaliya A, Jeong SH, Bhosale PB, Kim HH, Park MY, Kim E, Won CK, Park KI, Heo JD, Kim HW, et al. Mechanistic Action of Cell Cycle Arrest and Intrinsic Apoptosis via Inhibiting Akt/mTOR and Activation of p38-MAPK Signaling Pathways in Hep3B Liver Cancer Cells by Prunetrin—A Flavonoid with Therapeutic Potential. Nutrients. 2023; 15(15):3407. https://doi.org/10.3390/nu15153407
Chicago/Turabian StyleAbusaliya, Abuyaseer, Se Hyo Jeong, Pritam Bhagwan Bhosale, Hun Hwan Kim, Min Yeong Park, Eunhye Kim, Chung Kil Won, Kwang Il Park, Jeong Doo Heo, Hyun Wook Kim, and et al. 2023. "Mechanistic Action of Cell Cycle Arrest and Intrinsic Apoptosis via Inhibiting Akt/mTOR and Activation of p38-MAPK Signaling Pathways in Hep3B Liver Cancer Cells by Prunetrin—A Flavonoid with Therapeutic Potential" Nutrients 15, no. 15: 3407. https://doi.org/10.3390/nu15153407