Red-Fleshed Apple Flavonoids Extract Alleviates Male Reproductive Injury Caused by Busulfan in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design of the Study
2.2. Cell Experimental Protocol
2.3. Breeding Environment of Mice
2.4. Preparation and Extraction of Red-Fleshed Apple Flavonoids Extract (RAFEs)
2.5. Treatment of Mouse
2.6. Flow Cytometry Assay
2.7. Evaluation of Sperm Motility and Concentration
2.8. H&E and Immunofluorescence
2.9. TUNEL Assay of Apoptosis in Mouse
2.10. qRT-PCR
2.11. Western Blot Analysis
2.12. Statistical Analysis
3. Results
3.1. RAFEs Restore Spermatogonia Viability in Mouse
3.2. Effects of RAFEs on Weight of Mice Injected with Busulfan
3.3. RAFEs Modifies Biochemical Parameters in Mice
3.4. RAFEs Increases Sperm Motility and Sperm Concentration
3.5. Effects of RAFEs on Busulfan-Induced Damage to Different Tissues
3.6. RAFEs Improves the Expression of Important Genes Involved in Spermatogenesis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Control | CF1 | Busulfan | BF2 | BWF2 |
---|---|---|---|---|---|
Spermatogonia | 39.42 ± 3.42 ** | 42.49 ± 4.32 ** | 18.38 ± 2.11 ** | 35.22 ± 2.13 ** | 25.49 ± 2.40 |
Spermatocytes number | 164.24 ± 9.73 ** | 173.39 ± 10.48 ** | 38.58 ± 4.42 ** | 143.49 ± 8.82 ** | 69.32 ±4.29 * |
Leyding cells number | 24.31 ± 2.13 ** | 28.59 ± 3.98 ** | 7.24 ± 0.59 ** | 21.32 ± 1.34 ** | 10.46 ± 0.83 |
Seminiferous tubules diameter | 231.42 ± 6.42 ** | 252.38 ± 7.53 ** | 98.49 ± 2.49 ** | 194.47 ± 5.42 ** | 132.42 ± 2.68 ** |
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Wang, B.; Wang, Y.; Chen, Y.; Sun, X.; Xu, J.; Zhu, J.; Zhang, Y. Red-Fleshed Apple Flavonoids Extract Alleviates Male Reproductive Injury Caused by Busulfan in Mice. Nutrients 2023, 15, 3288. https://doi.org/10.3390/nu15153288
Wang B, Wang Y, Chen Y, Sun X, Xu J, Zhu J, Zhang Y. Red-Fleshed Apple Flavonoids Extract Alleviates Male Reproductive Injury Caused by Busulfan in Mice. Nutrients. 2023; 15(15):3288. https://doi.org/10.3390/nu15153288
Chicago/Turabian StyleWang, Bin, Yanbo Wang, Yizhou Chen, Xiaohong Sun, Jihua Xu, Jun Zhu, and Yugang Zhang. 2023. "Red-Fleshed Apple Flavonoids Extract Alleviates Male Reproductive Injury Caused by Busulfan in Mice" Nutrients 15, no. 15: 3288. https://doi.org/10.3390/nu15153288