Hematology Reports

4 pages, 666 KiB

Open AccessArticle

TET2 Promoter DNA Methylation and Expression Analysis in Pediatric B-Cell Acute Lymphoblastic Leukemia

by Ewa Musialik, Mateusz Bujko, Agnieszka Wypych, Michał Matysiak and Janusz Aleksander Siedlecki

Hematol. Rep. 2014, 6(1), 5333; https://doi.org/10.4081/hr.2014.5333 - 28 Mar 2014

Cited by 15 | Viewed by 481

TET2 is a novel tumor suppressor gene involved in several hematological malignancies of myeloid and lymphoid origin. Besides loss-of-function mutations and deletions, hypermethylation of the CpG island at the TET2 promoter was found in human cancer. Previous analysis revealed no TET2 mutations in acute lymphoblastic leukemia (ALL). Since the TET2 promoter methylation status in pediatric ALL has not been reported, the aim of the present study was to determine if promoter hypermethylation may be a mechanism of TET2 inactivation in a group of pediatric ALL cases. Methylation of TET2 promoter region in one (1/45) ALL B-common patient was detected by methylation specific polymerase chain reaction (PCR) and subsequently analyzed by bisulfite sequencing. We found no correlation between promoter methylation and gene expression, measured by quantitative reverse transcriptase-PCR, however the level of TET2 expression in ALL group was significantly decreased compared to children’s normal peripheral blood mononuclear cells and isolated B-cells. TET2 promoter hypermethylation seems to have limited clinical relevance in childhood B-cell ALL due to its low frequency. Full article

2 pages, 612 KiB

Open AccessCase Report

Intracranial Hemorrhage in Patient Treated with Rivaroxaban

by Michelle Molina, Virany H. Hillard and Robert Fekete

Hematol. Rep. 2014, 6(1), 5283; https://doi.org/10.4081/hr.2014.5283 - 28 Mar 2014

Cited by 6 | Viewed by 272

Abstract

Rivaroxaban is an oral factor Xa inhibitor used for stroke prevention in atrial fibrillation. There are currently no evidence-based guidelines for the treatment of hemorrhagic side effects of factor Xa inhibitors. We report a case of a thalamic hemorrhage in an 84 year-old right-handed female on rivaroxaban for treatment of atrial fibrillation. The patient had fallen down steps and became unresponsive. She was found to have diffuse scattered acute subarachnoid hemorrhage as well as intraventricular hemorrhage. Neurosurgical intervention was not required in this case, but controversy over decision making to pursue pro-coagulant therapy in the setting of worsening hemorrhage requiring emergent surgery is discussed. Full article

2 pages, 516 KiB

Open AccessCase Report

Glucose-6-Phosphate Dehydrogenase Deficiency Presented with Convulsion: A Rare Case

by Alparslan Merdin, Fatma Avci and Nihal Guzelay

Hematol. Rep. 2014, 6(1), 5266; https://doi.org/10.4081/hr.2014.5266 - 26 Mar 2014

Cited by 7 | Viewed by 271

Abstract

Red blood cells carry oxygen in the body and Glucose-6-Phosphate Dehydrogenase protects these cells from oxidative chemicals. If there is a lack of Glucose-6-Phosphate Dehydrogenase, red blood cells can go acute hemolysis. Convulsion is a rare presentation for acute hemolysis due to Glucose-6-Phosphate Dehydrogenase deficiency. Herein, we report a case report of a Glucose-6-Phosphate Dehydrogenase deficiency diagnosed patient after presentation with convulsion. A 70 year-old woman patient had been hospitalized because of convulsion and fatigue. She has not had similar symptoms before. She had ingested fava beans in the last two days. Her hypophyseal and brain magnetic resonance imaging were normal. Blood transfusion was performed and the patient recovered. Full article

5 pages, 835 KiB

Open AccessArticle

Serum Thrombopoietin and cMpl Expression in Thrombocytopenia of Different Etiologies

by Fabrizio Vianello, Silvia Vettore, Fabiana Tezza, Luca De Toni, Raffaella Scandellari, Luisa Sambado, Martina Treleani and Fabrizio Fabris

Hematol. Rep. 2014, 6(1), 4996; https://doi.org/10.4081/hr.2014.4996 - 26 Mar 2014

Cited by 6 | Viewed by 462

Abstract

The relationship between thrombopoietin (TPO) and its receptor cMpl in thrombocytopenic conditions has not been entirely clarified. To elucidate this interplay may expand the spectrum of indications of TPO mimetics. In this study we have explored the relationship between TPO and cMpl in platelets and megakaryocytes of 43 patients with thrombocytopenia due to idiopathic thrombocytopenic purpura (ITP), bone marrow hypoplasia, myelodysplastic syndromes (MDS), and familial thrombocytopenia. Data were compared to cMpl and TPO in patients with a normal platelet count and in patients with thrombocytosis due to essential thrombocythemia (ET). All but familial patients showed higher TPO compared to controls. All thrombocytopenic states were invariably associated with increased expression of platelet cMPL compared to healthy controls. ET patients showed normal TPO and a trend toward a reduced cMpl expression. Immunofluorescence of bone marrow sections from patients with ITP and MDS failed to show a peculiar pattern compared to controls. Multiple mechanisms regulate TPO and cMpl in thrombocytopenic conditions. Full article

3 pages, 685 KiB

Open AccessArticle

Isolated Hemolytic Anemia: An Unusual Manifestation of Occult Malignancy

by Matthew J. Butler, Ming Yin and Fahd Quddus

Hematol. Rep. 2014, 6(1), 5159; https://doi.org/10.4081/hr.2014.5159 - 05 Feb 2014

Cited by 1 | Viewed by 340

Abstract

Hemolysis is an uncommon and usually late complication of malignancy, and very rarely the presenting feature. Cancer-associated hemolysis may be immune-mediated, or may result from thrombotic microangiopathy accompanied by thrombocytopenia. We describe an unusual case of isolated hemolysis in the setting of occult metastatic breast cancer. The patient initially presented with symptomatic anemia, with evidence of hemolysis but with negative direct antiglobulin testing and a normal platelet count. Subsequent investigation discovered metastatic adenocarcinoma of the breast involving bone marrow. Hemolysis worsened despite initial treatment with cytotoxic chemotherapy and a trial of corticosteroids, but later resolved with aromatase inhibitor therapy. Full article

3 pages, 803 KiB

Open AccessCase Report

Reduced Intensity Allogeneic Stem Cell Transplant for Treatment of Blastic Plasmacytoid Dendritic Cell Neoplasm

by Anand Lokare, Emmanouil Nikolousis, Neil Phillips, Zbignieuw Rudzki, Richard Lovell, Bhuvan Kishore, Don Milligan and Shankara Paneesha

Hematol. Rep. 2014, 6(1), 5119; https://doi.org/10.4081/hr.2014.5119 - 29 Jan 2014

Cited by 4 | Viewed by 339

Abstract

Blastic plasmacytoid dendritic cell neoplasm is a rare, aggressive tumor characterized by skin and/or marrow infiltration by CD4⁺ CD56⁺ cells. Historically, the tumor was variably thought to arise from either monocytes, T cells or NK cells giving rise to terms such as CD4⁺/CD56⁺ acute monoblastic leukemia, primary cutaneous CD4⁺/CD56⁺ hematodermic tumor and blastic NK-cell lymphoma. Whilst considerable progress has been made in understanding the histogenesis, the best modality of treatment remains to be defined. We are therefore reporting this case which was successfully treated with a T-deplete allogeneic transplant and the patient is currently alive and in remission 4 years post transplant. Full article

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Hematol. Rep., Volume 6, Issue 1 (January 2014) – 6 articles

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