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Volume 13
Issue 10
10.3390/f13101578
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Article
Peer-Review Record

Genome-Wide Identification and Characterization of Calmodulin and Calmodulin-like Genes Family in Tea Plant and Their Roles under Abiotic Stress

Forests 2022, 13(10), 1578; https://doi.org/10.3390/f13101578
by Rui Kang 1,†, Renliang Zhao 1,†, Long Wang 2, Chunhui Liu 1, Fen Zhang 1 and Qiongqiong Zhou 1,*
Reviewer 1:
Baskar Venkidasamy
Reviewer 2:
Avinash Mishra
Forests 2022, 13(10), 1578; https://doi.org/10.3390/f13101578
Submission received: 16 August 2022 / Revised: 12 September 2022 / Accepted: 16 September 2022 / Published: 26 September 2022
(This article belongs to the Special Issue Dynamics of Upland Soil for Agroforestry Crops)

Round 1

Reviewer 1 Report

COMMENTS TO AUTHORS

The research MS presented by Kang et al., on " Genome-wide identification and characterization of calmodulin and calmodulin-like genes family in tea plant and their roles under abiotic stress" demonstrated the comprehensive genome wide analysis of calmodulin family genes in Tea plants. MS was written well and the work was carried out well. 

 

Comments:

1. It would be better if the author give an graphical abstract or workflow that describe the complete work

2. Genes and organisms should be shown in italics

3. Authors found the genes like CML1,14,33,52 were highly expressed in buds and the expression of CAM5 was higher in young leaves. Further, Cam33 expressed higher at low temperature. Often the young leaf and buds are used as tea production. Therefore, author could add few lines about the crosstalk between the expression of these genes and tea quality improvement and stress tolerance in discussion would be better.

 

 

Author Response

Please see the attachment

Author Response File: Author Response.docx

Reviewer 2 Report

Manuscript entitled “Genome-wide …….. abiotic stress” authored by Kang et al. presented the in silico identification of calmodulin or calmodulin like gene. Genome of Tea plant is available in public domain, authors did in silico gene mining and reported 5 CsCaM and 60 CsCML proteins using in silico tools. As lab experiment, they performed qRT-PCR of seven (out of 60) CML genes.

The study is very preliminary.

My few comments are as follows:–

1.       In section 2.4 they analyzed promoter (by in-silico means only): Authors are advised to characterized promoters by in-planta method.

2.       qRT-PCR: Are they used any stress? Or normal qRT

3.       Authors are advised to clone and functionally validate the best performing Ca or CLM gene

4.       Any report regarding CsPTB as house-keeping gene?

5.       Authors themselves agree in abstract that a number of calmodulins are reported from different plants and also fully functionalized. Why they did in-silico of tea plant ?

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 2 Report

Revision is satisfactory

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