Two-Stage Gene Therapy (VEGF, HGF and ANG1 Plasmids) as Adjunctive Therapy in the Treatment of Critical Lower Limb Ischemia in Diabetic Foot Syndrome
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Plasmid DNA
2.2. Patient Cohort
2.3. Administration of Plasmids
2.4. Clinical Evaluation
2.5. Ankle–Brachial Index (ABI)
2.6. Resting Pain
2.7. Transcutaneous Oxygen Pressure (TcPO2) Measurements
2.8. Statistical Analysis
3. Results
3.1. Clinical Follow-Up
3.2. ABI Results
3.3. PtcO2 Results
3.4. Resting Pain (VAS)
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Before Treatment (±SD) | After 3 Months (±SD) | p-Value | ||
---|---|---|---|---|
Wound classification (cm) | Group 1 | 1.80 ± 0.42 | 1.40 ± 0.52 | 0.037 * |
Group 2 (control) | 1.78 ± 0.44 | 1.67 ± 0.50 | 0.347 | |
ABI | Group 1 | 0.44 ± 0.14 | 0.47 ± 0.12 | 0.028 * |
Group 2 (control) | 0.46 ± 0.18 | 0.49 ± 0.17 | 0.175 | |
PtcO2 (mmHg) | Group 1 | 26.90 ± 8.99 | 33.9 ± 6.33 | 0.001 * |
Group 2 (control) | 28.71 ± 10.89 | 32.0 ± 8.81 | 0.058 | |
VAS | Group 1 | 6.80 ± 1.48 | 2.10 ± 1.10 | <0.001 * |
Group 2 (control) | 7.44 ± 1.42 | 3.78 ± 1.64 | <0.001 * |
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Barć, P.; Antkiewicz, M.; Frączkowska-Sioma, K.; Kupczyńska, D.; Lubieniecki, P.; Witkiewicz, W.; Małodobra-Mazur, M.; Baczyńska, D.; Janczak, D.; Skóra, J.P. Two-Stage Gene Therapy (VEGF, HGF and ANG1 Plasmids) as Adjunctive Therapy in the Treatment of Critical Lower Limb Ischemia in Diabetic Foot Syndrome. Int. J. Environ. Res. Public Health 2022, 19, 12818. https://doi.org/10.3390/ijerph191912818
Barć P, Antkiewicz M, Frączkowska-Sioma K, Kupczyńska D, Lubieniecki P, Witkiewicz W, Małodobra-Mazur M, Baczyńska D, Janczak D, Skóra JP. Two-Stage Gene Therapy (VEGF, HGF and ANG1 Plasmids) as Adjunctive Therapy in the Treatment of Critical Lower Limb Ischemia in Diabetic Foot Syndrome. International Journal of Environmental Research and Public Health. 2022; 19(19):12818. https://doi.org/10.3390/ijerph191912818
Chicago/Turabian StyleBarć, Piotr, Maciej Antkiewicz, Katarzyna Frączkowska-Sioma, Diana Kupczyńska, Paweł Lubieniecki, Wojciech Witkiewicz, Małgorzata Małodobra-Mazur, Dagmara Baczyńska, Dariusz Janczak, and Jan Paweł Skóra. 2022. "Two-Stage Gene Therapy (VEGF, HGF and ANG1 Plasmids) as Adjunctive Therapy in the Treatment of Critical Lower Limb Ischemia in Diabetic Foot Syndrome" International Journal of Environmental Research and Public Health 19, no. 19: 12818. https://doi.org/10.3390/ijerph191912818