Soil Bacterial Diversity and Its Relationship with Soil CO2 and Mineral Composition: A Case Study of the Laiwu Experimental Site
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Methods
2.2.1. Soil Sampling and Particle Size Analysis
2.2.2. The Method for Soil CO2 and Mineral Composition
2.2.3. Soil Bacterial Test and Analysis Method
- (1)
- DNA extraction and PCR amplification
- (2)
- Illumina MiSeq sequencing
- (3)
- Processing of sequencing data
3. Results
3.1. Soil CO2
3.2. Mineral Composition
3.3. Soil Bacterial Diversity
3.3.1. Rarefaction Curve of the Soil Bacterial Community
3.3.2. Venn Diagram Analysis of Species
3.3.3. Abundance of Bacterial Communities
3.3.4. OTU-Level Bacterial Diversity Analysis
4. Discussion
4.1. PCoA
4.2. RDA
4.3. Correlation Heatmap Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Chodak, M.; Gołębiewski, M.; Morawska-Płoskonka, J.; Kuduk, K.; Niklińska, M. Diversity of microorganisms from forest soils differently polluted with heavy metals. Appl. Soil Ecol. 2013, 64, 7–14. [Google Scholar] [CrossRef]
- Han, X.; Wang, R.; Liu, J.; Wang, M.; Zhou, J.; Guo, W. Effects of vegetation type on soil microbial community structure and catabolic diversity assessed by polyphasic methods in North China. J. Environ. Sci. 2007, 19, 1228–1234. [Google Scholar] [CrossRef]
- Bi, J.; He, D.; Sha, Y.; Huang, Z. Functional diversity of soil microbial community under different types of vegetation in the desert grassland. Agric. Res. Arid Areas 2009, 27, 149–155. [Google Scholar]
- Marschner, P.; Yang, C.H.; Lieberei, R.; Crowley, D.E. Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biol. Biochem. 2001, 33, 1437–1445. [Google Scholar] [CrossRef]
- Rinnan, R.; Stark, S.; Tolvanen, A. Responses of Vegetation and Soil Microbial Communities to Warming and Simulated Herbivory in a Subarctic Heath. J. Ecol. 2010, 97, 788–800. [Google Scholar] [CrossRef]
- Yan, J.; Hang, X.; Wang, S. Variation of soil microoganism under different vegetation coverages and fertilization systems in black soil. Chin. J. Soil Sci. 2009, 40, 240–244. [Google Scholar]
- Xing, P.; Wu, X.; Gao, S.; Li, H.; Zhao, T.; Zhou, X.; Shen, D.; Sun, J. Effect of different fertillization on soil microbial community and functional diversity in Maize-Wheat crop rotation. J. Microbiol. 2016, 36, 22–29. [Google Scholar]
- Waldrop, M.P.; Balser, T.C.; Firestone, M.K. Linking microbial community composition to function in a tropical soil. Soil Biol. Biochem. 2000, 32, 1837–1846. [Google Scholar] [CrossRef]
- Gao, Y.; Qi, Z.; Zhong, Q.; Fan, T.; Li, S.; Wang, K.; Zhu, H.; Zhou, T. Responses of soil microbial biomass to long-term simulated warming in Eastern Chongming Island wetlands, China. Acta Ecol. Sin. 2018, 38, 711–720. [Google Scholar]
- Guo, X.; Chen, H.Y.H.; Meng, M.; Biswas, S.R.; Ye, L.; Zhang, J. Effects of land use change on the composition of soil microbial communities in a managed subtropical forest. For. Ecol. Manag. 2016, 373, 93–99. [Google Scholar] [CrossRef]
- Dick, R.P.; Rasmussen, P.E.; Kerle, E.A. Influence of long-term residue management on soil enzyme activities in relation to soil chemical properties of a wheat-fallow system. Biol. Fertil. Soils 1988, 6, 159–164. [Google Scholar] [CrossRef]
- Pang, B.; Yu, Y. Analysis on soil microbial community in different land use types on reclamation of coal waste pile. Chin. J. Soil Sci. 2017, 48, 359–364. [Google Scholar]
- Xiaoli, F.; Fengting, Y.; Jianlei, W.; Yuebao, D.; Xiaoqin, D.; Xinyu, Z.; Huimin, W. Understory vegetation leads to changes in soil acidity and in microbial communities 27 years after reforestation. Sci. Total Environ. 2015, 502, 280–286. [Google Scholar]
- Li, X.; Jing, S.; Wang, H.; Xu, L.; Jian, W.; Zhang, H. Changes in the soil microbial phospholipid fatty acid profile with depth in three soil types of paddy fields in China. Geoderma 2017, 290, 69–74. [Google Scholar] [CrossRef]
- Hong, P.; Liu, S.; Wang, H.; Yu, H. Characteristics of soil microbial community structure in two young plantations of Castanopsis hystrix and Erythrophleum fordii in subtropical China. Acta Ecol. Sin. 2016, 36, 4496–4508. [Google Scholar]
- Xia, B.; Zhou, J.; Tiedje, J.M. Effect of vegetation on structure of soil microbial community. Chin. J. Appl. Ecol. 1998, 9, 296–300. [Google Scholar]
- Ward, D.M.; Bateson, M.M.; Weller, R.; Ruffroberts, A.L. Ribosomal RNA Analysis of Microorganisms as They Occur in Nature. Adv. Microb. Ecol. 1992, 12, 219–286. [Google Scholar]
- Johnson, M.J.; Lee, K.Y.; Scow, K.M. DNA fingerprinting reveals links among agricultural crops, soil properties, and the composition of soil microbial communities. Geoderma 2003, 114, 279–303. [Google Scholar] [CrossRef]
- Lienhard, P.; Terrat, S.; Mathieu, O.; Levêque, J.; Prévost-Bouré, N.C.; Nowak, V.; Régnier, T.; Faivre, C.; Sayphoummie, S.; Panyasiri, K. Soil microbial diversity and C turnover modified by tillage and cropping in Laos tropical grassland. Environ. Chem. Lett. 2013, 11, 391–398. [Google Scholar] [CrossRef]
- Yang, Y.; Dou, Y.; An, S. Testing association between soil bacterial diversity and soil carbon storage on the Loess Plateau. Sci. Total. Environ. 2018, 626, 48. [Google Scholar] [CrossRef]
- Zhao, N.N.; Guggenberger, G.; Shibistova, O.; Thao, D.T.; Wen, J.S.; Xiao, G.L. Aspect-vegetation complex effects on biochemical characteristics and decomposability of soil organic carbon on the eastern Qinghai-Tibetan Plateau. Plant Soil 2014, 384, 289–301. [Google Scholar] [CrossRef]
- Deng, S.; Lin, M.; Li, F.; Su, Y.; Liu, K. Effect of fertilization on soil carbon pool management indes and enzyme activities in pasture grown soil of the Karst region. Acta Pedol. Sin. 2014, 23, 262–268. [Google Scholar]
- Thevenot, M.; Dignac, M.F.; Rumpel, C. Fate of lignins in soils: A review. Soil Biol. Biochem. 2010, 42, 1200–1211. [Google Scholar] [CrossRef]
- Lange, M.; Eisenhauer, N.; Sierra, C.A.; Bessler, H.; Engels, C.; Griffiths, R.I.; Melladovázquez, P.G.; Malik, A.A.; Roy, J.; Scheu, S. Plant diversity increases soil microbial activity and soil carbon storage. Nat. Commun. 2015, 6, 6707. [Google Scholar] [CrossRef]
- Si, G.; Yuan, J.; Xu, X.; Zhao, S.; Peng, C.; Wu, J.; Zhou, Z. Effects of an integrated rice-crayfish farming system on soil organic carbon, enzyme activity, and microbial diversity in waterlogged paddy soil. Acta Ecol. Sin. 2018, 38, 29–35. [Google Scholar] [CrossRef]
- Cheng, M.; Zhu, Q.; Liu, L.; An, S. Effects of vegetation on soil aggregate stability and organic carbon sequestration in the Ningxia Loess Hilly Region of northwest China. Acta Ecol. Sin. 2013, 33, 2835–2844. [Google Scholar] [CrossRef] [Green Version]
- Grover, M.; Maheswari, M.; Desai, S.; Gopinath, K.A.; Venkateswarlu, B. Elevated CO2: Plant associated microorganisms and carbon sequestration. Appl. Soil Ecol. 2015, 95, 73–85. [Google Scholar] [CrossRef]
- Yu, Z.; Wang, Y.; Yan, N.; Li, Y.; Xie, Z.; Jin, J. Effect of elevated CO2 on the abundance of rhizosphere bacteria, fungi and denitrification bacteria in diffent soybean cultivars. Soil Crops 2017, 6, 9–16. [Google Scholar]
- Celine, L.; Dimitris, P.; Sean, M.C.; Bernard, O.; Steven, S.; Safiyh, T.; Donald, Z.; Daniel, V.D.L. Elevated atmospheric CO2 affects soil microbial diversity associated with trembling aspen. Environ. Microbiol. 2010, 10, 926–941. [Google Scholar]
- Yuan, L.; Mao, L.; Zheng, J.; Li, L.; Zhang, X.; Zheng, J.; Pan, G.; Yu, X.; Wang, J. Short-term responses of microbial community and functioning to experimental CO2 enrichment and warming in a Chinese paddy field. Soil Biol. Biochem. 2014, 77, 58–68. [Google Scholar]
- Nannipieri, P.; Ascher, J.; Ceccherini, M.T.; Landi, L.; Pietramellara, G.; Renella, G. Microbial diversity and soil fuctions. Eur. J. Soil Sci. 2003, 54, 665–670. [Google Scholar] [CrossRef]
- Huang, Q.; Wu, J.; Chen, W.; Li, X. Adsorption of Cadmium by Soil Colloids and Minerals in Presence of Rhizobia. Pedosphere 2000, 10, 299–307. [Google Scholar]
- Brown, D.A.; Sherriff, B.L.; Sawicki, J.A. Microbial transformation of magnetite to hematite. Geochim. Cosmochim. Acta 1997, 61, 3341–3348. [Google Scholar] [CrossRef]
- Deng, Y.; Zheng, T.; Wang, Y.; Liu, L.; Jiang, H.; Ma, T. Effect of microbially mediated iron mineral transformation on temporal variation of arsenic in the Pleistocene aquifers of the central Yangtze River basin. Sci. Total Environ. 2018, 619–620, 1247–1258. [Google Scholar] [CrossRef]
- Certini, G.; Campbell, C.; Edwards, A. Rock fragments in soil support a different microbial community from the fine earth. Soil Biol. Biochem. 2004, 36, 1119–1128. [Google Scholar] [CrossRef]
- Javed, I.; Hu, R.; Feng, M.; Lin, S.; Saadatullah, M.; Ibrahimmohamed, A. Microbial biomass, and dissolved organic carbon and nitrogen strongly affect soil respiration in different land uses: A case study at Three Gorges Reservoir Area, South China. Agric. Ecosyst. Environ. 2010, 137, 294–307. [Google Scholar] [CrossRef]
- Wang, H.; Guo, H.; Xiu, W.; Jonas, B.; Sun, G.; Tang, X.; Setefan, N. Indications that weathering of evaporite minerals affects groundwater salinity and As mobilization in aquifers of the northwestern Hetao Basin, China. Appl. Geochem. 2019, 109. [Google Scholar] [CrossRef]
- Xia, L.; Gao, Z.; Zheng, X.; Wei, J. Impact of recharge water temperature on bioclogging during managed aquifer recharge: A laboratory study. Hydrogeol. J. 2018, 26, 2173–2187. [Google Scholar] [CrossRef]
- Ibekwe, A.M.; Gonzalez-Rubio, A.; Suarez, D.L. Impact of treated wastewater for irrigation on soil microbial communities. Sci. Total. Environ. 2018, 622–623, 1603–1610. [Google Scholar] [CrossRef]
- Ren, C.; Zhang, W.; Zhong, Z.; Han, X.; Yang, G.; Feng, Y.; Ren, G. Differential responses of soil microbial biomass, diversity, and compositions to altitudinal gradients depend on plant and soil characteristics. Sci. Total Environ. 2018, 610–611, 750–758. [Google Scholar] [CrossRef]
- Shao, P.; Liang, C.; Lynch, L.; Xie, H.; Bao, X. Reforestation accelerates soil organic carbon accumulation: Evidence from microbial biomarkers. Soil Biol. Biochem. 2019, 131, 182–190. [Google Scholar] [CrossRef]
- Wu, W.; Dong, C.; Wu, J.; Liu, X.; Wu, Y.; Chen, X.; Yu, S. Ecological effects of soil properties and metal concentrations on the composition and diversity of microbial communities associated with land use patterns in an electronic waste recycling region. Sci. Total Environ. 2017, 601–602, 57–65. [Google Scholar] [CrossRef] [PubMed]
- Arora, B.; Spycher, N.F.; Steefel, C.I.; Molins, S.; Bill, M.; Conrad, M.E.; Dong, W.; Faybishenko, B.; Tokunaga, T.K.; Wan, J. Influence of hydrological, biogeochemical and temperature transients on subsurface carbon fluxes in a flood plain environment. Biogeochemistry 2016, 127, 1–30. [Google Scholar] [CrossRef] [Green Version]
- Conrad, M.; Arora, B.; Williams, K.; Bill, M.; Spycher, N.; Steefel, C.; Tokunaga, T.; Hubbard, S. Using Concentrations and Isotopic Compositions of CO2 to Distinguish Microbial Production of CO2 in Unsaturated Zone Sediments in Hydrogeochemical Models. In Proceedings of the Agu Fall Meeting, San Francisco, CA, USA, 15–19 December 2014. [Google Scholar]
- Davidson, E.; Lefebvre, P.A.; Brando, P.M.; Ray, D.M.; Trumbore, S.E.; Solorzano, L.A.; Ferreira, J.N.; Bustamante, M.M.D.C.; Nepstad, D.C. Carbon Inputs and Water Uptake in Deep Soils of an Eastern Amazon Forest. For. Sci. 2011, 57, 51–58. [Google Scholar]
- Walvoord, M.A.; Striegl, R.G.; Prudic, D.E.; Stonestrom, D.A. CO2 dynamics in the Amargosa Desert: Fluxes and isotopic speciation in a deep unsaturated zone. Water Resour. Res. 2005, 41, 189–205. [Google Scholar] [CrossRef]
- Johnson, M.S.; Lehmann, J.; Riha, S.J.; Krusche, A.V.; Couto, E.G. CO2 efflux from Amazonian headwater streams represents a significant fate for deep soil respiration. Geophys. Res. Lett. 2007, 35, L17401. [Google Scholar] [CrossRef]
- Hendry, M.J.; Mendoza, C.A.; Kirkland, R.A.; Lawrence, J.R. Quantification of transient CO2 production in a sandy unsaturated zone. Water Resour. Res. 1999, 35, 2189–2198. [Google Scholar] [CrossRef]
- Zhang, H.; Gao, Z.; Shi, M.; Fang, S.; Xu, H.; Cui, Y.; Liu, J. Study of the Effects of Land Use on Hydrochemistry and Soil Microbial Diversity. Water 2019, 11, 466. [Google Scholar] [CrossRef] [Green Version]
- Zhuang, J.; Yu, G. Effects of surface coatings on electrochemical properties and contaminant sorption of clay minerals. Chemosphere 2002, 49, 619–628. [Google Scholar] [CrossRef]
- Zhang, M.; Zhang, Y.; Li, X.; Wang, J.; Guanglu, M.; Shi, B. Mineral compositions of soil in the arid and semiarid region and their environmental significance. J. Lanzhou Univ. (Nat. Sci.) 2007, 43, 1–7. [Google Scholar]
- Beaufort, L.; Beaufort, D.; Berger, G.; Bauer, A.; Cassagnabère, A.; Meunier, A. Authigenic kaolin and illitic minerals during burial diagenesis of sandstones: A review. Clay Miner. 2002, 37, 1–22. [Google Scholar]
- White, A.; Schulz, M.; Bullen, T.; Fitzpatrick, J.; Vivit, D.; Evett, R.; Tipper, E.; Gilkes, R.J.; Prakongkep, N. Use of elemental and isotopic ratios to distinguish between lithogenic and biogenic sources of soil mineral nutrients. In Proceedings of the World Congress of Soil Science: Soil Solutions for A Changing World, Brisbane, QLD, Australia, 1–6 August 2010. [Google Scholar]
- Yang, L.; Yue, L.; Li, Z. The influence of dry lakebeds, degraded sandy grasslands and abandoned farmland in the arid inlands of northern China on the grain size distribution of East Asian aeolian dust. Environ. Geol. 2008, 53, 1767–1775. [Google Scholar] [CrossRef]
- Yu, Z.; Liu, J.; Li, Y.; Jin, J.; Liu, X.; Wang, G. Impact of land use, fertilization and seasonal variation on the abundance and diversity of nirS-type denitrifying bacterial communities in a Mollisol in Northeast China. Eur. J. Soil Biol. 2018, 85, 4–11. [Google Scholar] [CrossRef]
- Yang, Y.; Tilman, D.; Furey, G.; Lehman, C. Soil carbon sequestration accelerated by restoration of grassland biodiversity. Nat. Commun. 2019, 10, 718. [Google Scholar] [CrossRef] [Green Version]
- Rong, X.; Huang, Q.; He, X.; Chen, H.; Cai, P.; Liang, W. Interaction of Pseudomonas putida with kaolinite and montmorillonite: A combination study by equilibrium adsorption, ITC, SEM and FTIR. Colloids Surf. B Biointerfaces 2008, 64, 49–55. [Google Scholar] [CrossRef]
- Valboa, G.; Lagomarsino, A.; Brandi, G.; Agnelli, A.E.; Simoncini, S. Long-term variations in soil organic matter under different tillage intensities. Soil Tillage Res. 2015, 154, 126–135. [Google Scholar] [CrossRef]
- Heinze, S.; Ludwig, B.; Piepho, H.-P.; Mikutta, R.; Don, A.; Wordell-Dietrich, P.; Helfrich, M.; Hertel, D.; Leuschner, C.; Kirfel, K. Factors controlling the variability of organic matter in the top- and subsoil of a sandy Dystric Cambisol under beech forest. Geoderma 2018, 311, 37–44. [Google Scholar] [CrossRef]
- Liu, H.; Yuan, P.; Liu, D.; Bu, H.; Song, H.; Qin, Z.; He, H. Pyrolysis behaviors of organic matter (OM) with the same alkyl main chain but different functional groups in the presence of clay minerals. Appl. Clay Sci. 2018, 153, 205–216. [Google Scholar] [CrossRef]
- Wang, Y.; Liu, X.; Si, Y.; Wang, R. Release and transformation of arsenic from As-bearing iron minerals by Fe-reducing bacteria. Chem. Eng. J. 2016, 295, 29–38. [Google Scholar] [CrossRef]
- Müller, T.M.; Höper, H.H. Soil organic matter turnover as a function of the soil clay content: Consequences for model applications. Soil Biol. Biochem. 2004, 36, 877–888. [Google Scholar] [CrossRef]
- Barker, W.W.; Welch, S.A.; Chu, S.; Banfield, J.F. Experimental observations of the effects of bacteria on aluminosilicate weathering. Am. Mineral. 1998, 83, 1551–1563. [Google Scholar] [CrossRef]
- Uroz, S.; Calvaruso, C.; Turpault, M.P.; Pierrat, J.C.; Mustin, C.; Frey-Klett, P. Effect of the Mycorrhizosphere on the Genotypic and Metabolic Diversity of the Bacterial Communities Involved in Mineral Weathering in a Forest Soil. Appl. Environ. Microbiol. 2007, 73, 3019–3027. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhou, W.; Yan, M.; Su, C.; Li, L.; Lei, Z. Study on soil microbial quantity and biomass developed from different carbonate-rock and soil thickness: A case study of Huaxi districtin Guiyang. Carsologica Sin. 2018, 37, 168–174. [Google Scholar] [CrossRef]
- Amaro, T.; Bertocci, I.; Queiros, A.; Rastelli, E.; Borgersen, G.; Brkljacic, M.; Nunes, J.; Sorensen, K.; Danovaro, R.; Widdicombe, S. Effects of sub-seabed CO2 leakage: Short- and medium-term responses of benthic macrofaunal assemblages. Mar. Pollut. Bull. 2018, 128, 519–526. [Google Scholar] [CrossRef] [PubMed]
- Guenet, B.; Lenhart, K.; Leloup, J.; Giusti-Miller, S.; Pouteau, V.; P, M.; Nunan, N.; Abbadie, L. The impact of long-term CO2 enrichment and moisture levels on soil microbial community structure and enzyme activities. Geoderma 2012, 170. [Google Scholar] [CrossRef]
- Iv, F.; Billings, S. Model formulation of microbial CO2 production and efficiency can significantly influence short and long term soil C projections. ISME J. 2018, 12. [Google Scholar] [CrossRef] [Green Version]
- Feng, Y.; Lin, X.; Zhang, J.; Mao, T.; Zhu, J. Soil purple phototrophic bacterial diversity under double cropping (rice-wheat) with free-air CO2 enrichment (FACE). Eur. J. Soil Sci. 2011, 62, 533–540. [Google Scholar] [CrossRef]
- Dunbar, J.; Gallegos-Graves, L.; Steven, B.; Mueller, R.; Hesse, C.; Zak, D.; Kuske, C. Surface soil fungal and bacterial communities in aspen stands are resilient to eleven years of elevated CO2 and O3. Soil Biol. Biochem. 2014, 76, 227–234. [Google Scholar] [CrossRef]
- Drigo, B.; Kowalchuk, G.A.; Veen, J.A.V. Climate change goes underground: Effects of elevated atmospheric CO2 on microbial community structure and activities in the rhizosphere. Biol. Fertil. Soils 2008, 44, 667–679. [Google Scholar] [CrossRef] [Green Version]
Sample | W01 | W02 | W03 | L01 | L02 | L03 | L04 | L05 |
---|---|---|---|---|---|---|---|---|
Land use type | Bare | Bare | Bare | Bare | Peanut | Peach | Chestnut | Pine |
Depth (cm) | 0–15 | 30–45 | 60–75 | 0–15 | 0–15 | 0–15 | 0–15 | 0–15 |
Samples | Quartz | K-Feldspar | Plagioclase | Calcite | Clay Minerals | Amphibole |
---|---|---|---|---|---|---|
L01 (W01) | 27.4 | 29.9 | 33.7 | 1.3 | 7.1 | 0.5 |
L02 | 26.2 | 21.2 | 42.8 | 1.2 | 7.9 | 0.8 |
L03 | 19.2 | 27.7 | 42.3 | 1.1 | 8.2 | 1.5 |
L04 | 24.7 | 23.0 | 38.7 | 1.6 | 10.4 | 1.5 |
L05 | 26.5 | 19.6 | 40.6 | 1.2 | 10.0 | 2.0 |
W02 | 22.2 | 22.4 | 46.7 | 1.1 | 6.2 | 1.4 |
W03 | 21.0 | 26.0 | 42.2 | 1.4 | 7.2 | 2.2 |
Sample | Shannon | Simpson | ACE | Chao1 |
---|---|---|---|---|
W01 | 5.876 | 0.006062 | 997.07662 | 1013.0323 |
W02 | 5.860 | 0.005901 | 1031.5267 | 1028.75 |
W03 | 5.917 | 0.005626 | 1094.331 | 1096.1724 |
L01 | 6.020 | 0.004679 | 1280.969 | 1301.324 |
L02 | 4.606 | 0.060254 | 860.9973 | 858.8333 |
L03 | 5.990 | 0.005436 | 1354.22 | 1379.128 |
L04 | 5.989 | 0.005554 | 1325.689 | 1332.94 |
L05 | 5.667 | 0.017638 | 1316.49 | 1332.898 |
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Zhang, H.; Gao, Z.; Shi, M.; Fang, S. Soil Bacterial Diversity and Its Relationship with Soil CO2 and Mineral Composition: A Case Study of the Laiwu Experimental Site. Int. J. Environ. Res. Public Health 2020, 17, 5699. https://doi.org/10.3390/ijerph17165699
Zhang H, Gao Z, Shi M, Fang S. Soil Bacterial Diversity and Its Relationship with Soil CO2 and Mineral Composition: A Case Study of the Laiwu Experimental Site. International Journal of Environmental Research and Public Health. 2020; 17(16):5699. https://doi.org/10.3390/ijerph17165699
Chicago/Turabian StyleZhang, Hongying, Zongjun Gao, Mengjie Shi, and Shaoyan Fang. 2020. "Soil Bacterial Diversity and Its Relationship with Soil CO2 and Mineral Composition: A Case Study of the Laiwu Experimental Site" International Journal of Environmental Research and Public Health 17, no. 16: 5699. https://doi.org/10.3390/ijerph17165699