Current Issues in Molecular Biology

644 KiB

Open AccessReview

Detection of Single Nucleotide Polymorphisms

by Pui-Yan Kwok and Xiangning Chen

Curr. Issues Mol. Biol. 2003, 5(2), 43-60; https://doi.org/10.21775/cimb.005.043 - 01 Apr 2003

Cited by 1 | Viewed by 3740

Single nucleotide polymorphism (SNP) detection technologies are used to scan for new polymorphisms and to determine the allele(s) of a known polymorphism in target sequences. SNP detection technologies have evolved from labor intensive, time consuming, and expensive processes to some of the most highly automated, efficient, and relatively inexpensive methods. Driven by the Human Genome Project, these technologies are now maturing and robust strategies are found in both SNP discovery and genotyping areas. The nearly completed human genome sequence provides the reference against which all other sequencing data can be compared. Global SNP discovery is therefore only limited by the amount of funding available for the activity. Local, target, SNP discovery relies mostly on direct DNA sequencing or on denaturing high performance liquid chromatography (dHPLC). The number of SNP genotyping methods has exploded in recent years and many robust methods are currently available. The demand for SNP genotyping is great, however, and no one method is able to meet the needs of all studies using SNPs. Despite the considerable gains over the last decade, new approaches must be developed to lower the cost and increase the speed of SNP detection. Full article

544 KiB

Open AccessReview

Genomes and Evolution

by Antoine Danchin

Curr. Issues Mol. Biol. 2003, 5(2), 37-42; https://doi.org/10.21775/cimb.005.037 - 01 Apr 2003

Viewed by 353

Abstract

Genomics today involves the study of many genes at a time in order to gain an integrated picture of the cell or organism as a whole. This review considers the architecture and evolution of bacterial genomes. The many facets of large-scale functional investigation in a variety of bacteria and the search to find common rules in their dynamic and structural organization are discussed. Such rules could aid the understanding of common properties and essential differences corresponding to elusive functions, or of still unknown bacterial biotopes. Full article

686 KiB

Open AccessReview

Handling of Clinical Tissue Specimens for Molecular Profiling Studies

by Isabel M. Leiva, Michael R. Emmert-Buck and John W. Gillespie

Curr. Issues Mol. Biol. 2003, 5(2), 27-35; https://doi.org/10.21775/cimb.005.027 - 01 Apr 2003

Viewed by 341

Abstract

The relationship between gene expression profiles and cellular phenotypes is an important aspect of functional genomics. Clinical tissue specimens will play a vital role in this effort. The usefulness of tissue for molecular profiling is significantly influenced by the manner of specimen handling. Crucial components of this process include the optimization of the methods of tissue fixation and embedding, not only to obtain excellent histological detail, but also to promote the elucidation of the gene and protein expression profiles. In this article, we describe handling of clinical specimens using whole-mount prostate as an example, the use of new high-throughput techniques that allow molecular profiling analysis and the use of a web-based 3-dimensional model to combine these data to make it available to clinicians and the research community. Complete protocols and additional discussion are available on the website, http://cgapmf. nih.gov. Full article

Journal Menu

Journal Browser

Curr. Issues Mol. Biol., Volume 5, Issue 2 (April 2003) – 3 articles

Further Information

Guidelines

MDPI Initiatives

Follow MDPI