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Communication

Three-Step Synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide from 4,7-Dichloroquinoline

by
Deiby F. Aparicio Acevedo
,
Marlyn C. Ortiz Villamizar
and
Vladimir V. Kouznetsov
*
Laboratorio de Química Orgánica y Biomolecular, Escuela de Química, Universidad Industrial de Santander, Cl. 9 # Cra 27, A.A., Bucaramanga 680006, Colombia
*
Author to whom correspondence should be addressed.
Molbank 2024, 2024(1), M1796; https://doi.org/10.3390/M1796
Submission received: 5 March 2024 / Revised: 15 March 2024 / Accepted: 16 March 2024 / Published: 21 March 2024
(This article belongs to the Section Organic Synthesis)
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Abstract

:
The quinoline derivative, N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide, was synthesized in a conventional three-step procedure from 4,7-dichloroquinoline using a N-oxidation reaction/C2-amide formation reaction/C4 SNAr reaction sequence. The structure of the compound was fully characterized by FT-IR, 1H-, 13C-NMR, DEPT-135°, and ESI-MS techniques. Its physicochemical parameters (Lipinski’s descriptors) were also calculated using the online SwissADME database. Such derivatives are relevant therapeutic agents exhibiting potent anticancer, antibacterial, antifungal, and antiparasitic properties.

Graphical Abstract

1. Introduction

Quinoline and morpholine are favored molecular frameworks for medicinal and advanced material chemistry [1,2,3,4,5,6]. (Quinolinyl)amides and N-(quinolinyl)morpholines are especially interesting biological active molecules that can serve as useful scaffolds in pharmaceutical research. They can act as human vanilloid receptor type 1 (TRPV1) antagonists and melanin-concentrating hormone 1 receptor (MCH1R) antagonists [7,8,9], as well as antifungal, antibacterial, and trypanocidal agents [10,11,12,13]. In particular, N-(quinolin-2-yl)benzamide derivative A (Figure 1), designed and synthesized as an anticancer sorafenib congener, presented interesting antiproliferative activity (HCT-116, MCF-7, and SK-BR3 cancer cell lines) [14]. On the other hand, 4-(6-amidoquinolin-2-yl)morpholine B is a potent MCH1R antagonist, implicated in body weight (obesity) regulation, a major contributor to the development of diseases, including type 2 diabetes mellitus, coronary heart disease, certain forms of cancer, and osteoarthritis [9]. Additionally, 4-(quinolin-4-yl)morpholine C exhibited some in vitro antibacterial activity [15].
Additionally, pincer palladium complexes, based on the N-(quinolin-8-yl)amide ring, have been widely explored for diverse catalytic transformations [16], and 4,7-dichloroquinoline has been extensively used in drug and functional material research as an affordable chemical intermediate for numerous bioactive functionalized 4-amino-quinoline compounds [17,18,19,20].
Due to the paramount significance of the functionalized quinoline core in the search for drug candidates and medicinal chemistry, the development of new synthetic methodologies remains an active area. Among modern methodologies, there is regioselective functionalization of the quinoline ring through C-H activation, especially via N-oxide formation, using it as a directing group [21,22]. The latter is considered one of the most powerful and, thus, attractive approaches for synthesizing C-2 and C-8 functionalized quinoline compounds under mild reaction conditions [23,24,25,26]. In particular, the metal-free deoxygenative 2-amidation of quinoline N-oxides is one of the most promising constructions of N-acylated 2-aminoquinolines, in which amides and nitriles are used as the amide formation reagents in the presence of several promoters, i.e., oxalyl chloride, trifluoroacetic anhydride/2-fluoropyridine mixture, TsOH·H2O or selectfluor-methylcarbazate (NH2NHCO2Me) combination, and under Brønsted acidic ionic liquid (BAIL, [BSmim][OTf]) medium [27,28,29,30,31]. Such approaches constitute a paradigm in diversity-oriented synthesis strategies, permitting the late-stage diversification of key pharmaceutical scaffolds [32].
Considering the above-stated aspects and as a continuation of our efforts to prepare new bioactive 7-chloroquinoline-based molecules [33,34,35,36,37,38], we designed, prepared, and characterized the above-mentioned N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide. Therefore, this work describes a practical, efficient method for synthesizing the title compound through the N-oxidation reaction/C2-amidation reaction/C4 SNAr reaction sequence using commercially accessible reagents.

2. Results and Discussion

The N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide (4) was easily prepared through a conventional three-step procedure from commercially available 4,7-dichloroquinoline (1), which was oxidized with a 1.2 equivalent of m-chloroperbenzoic acid (m-CPBA) in CHCl3 at room temperature for 5 h to give N-oxide 2 [39] in an 81% yield (Scheme 1).
This N-oxide was also obtained in an 87% yield using acetic acid and 30% hydrogen peroxide mixture [40] as an oxidant reagent at 70 °C for 1 h. The reaction of 4,7-dichloroquinoline 1-oxide (2) with benzonitrile proceeded to reflux dichloromethane and conc. sulfuric acid at 70 °C for 24 h with deoxygenation to provide N-(quinolin-2-yl)benzamide 3 in a 92% yield (a key C2 amide formation process). Note that the conditions reported by Chen and coworkers (TsOH·H2O, 150 °C, 12 h) to adopt C-H functionalization via C2 amide formation reaction [29] failed in our hands. The final step of the developed method is the nucleophilic aromatic substitution (SNAr) involving the regioselective displacement of the C4-chlorine atom in quinoline compound 3 to obtain titled quinoline 4 in a 92% yield. This was achieved through a base-promoted amination [41], easily realized by reacting 4-chloroquinoline derivative 3 and morpholine in the presence of K2CO3 and refluxing DMF for 24 h (Scheme 1). This protocol is valuable for large-scale production due to its simplicity of operation, transition metal-free conditions, and scalability.
The structural elucidation of intermediate 2 and final compounds 3–4 was achieved based on spectroscopic data, and the results are presented in the experimental section and the electronic supporting information (ESI). Their infrared spectra record the absorption bands associated with the functional groups present in their structure. Characteristic amide -C=O strain vibration appeared in 1693 and 1674 cm−1, respectively, for compounds 3 and 4, while the band at 1287 cm−1, corresponding to the tension of the N-O bond of precursor 2, is not present, which confirms a successful deoxygenated amidation procedure of 2 to form 3 (ESI, Figures S1–S3). This C-2 functionalization process is also indirectly corroborated by the absence of the signal of proton H2 in the 1H NMR spectra of quinolines 3–4; meanwhile, the doublet-shaped signal centered at 8.43 ppm with a coupling constant of 6.6 Hz corresponds to the hydrogen that is in position 2 of the quinoline ring, observed in the proton spectra of N-oxide 2 (Figures S7, S9, and S11). Each product 3–4 formation was further verified by its mass spectra. The electrospray ionization (ESI) technique in the positive mode of the obtained N-(quinolin-2-yl) benzamides 3–4 allowed us to observe the molecular ion peak [M+H+] at m/z 319.0/317.0 and 370.1/368.1 for the C16H10Cl2N2O and C20H18ClN3O2 molecular formulae, exhibiting the isotopic contribution of 37Cl in ratios of ~1:2 and 1:3 (37Cl/35Cl), respectively (Figures S5 and S6).
The 13C-NMR and DEPT-135° spectra, shown in Figures S8, S10, and S12, were analyzed to complete the characterization. Each hydrogen and carbon atom signal was assigned by carefully analyzing the homonuclear and heteronuclear correlation spectra (COSY, HMBC, and HSQC experiments) (Figures S13–S17).
According to previous literature reports [29,42,43], a plausible reaction mechanism of the key C2 amide formation process could be proposed. Nitrilium ion B is generated in situ from benzonitrile A with sulfuric acid. The formed ion could react with N-oxide 2 as a dipolarophile via the [3+2]-dipolar cycloaddition to afford the five-membered oxadiazolidine intermediate D. It could also act as an electrophilic reagent through nucleophilic addition to form the Reissert-type intermediate C. The latter is susceptible to intramolecular nucleophilic attack, producing the intermediate D. It then undergoes rearomatization with bond rupture (N-O) and a proton loss to form the stable product 3 (Scheme 2).
However, the low reactivity of such N-oxides as a 1,3-dipole in the cycloaddition reaction [43] makes the direct formation of intermediate D less possible. Thus, it is believed that its formation via the trapping of highly electrophilic intermediate B with weakly nucleophilic N-oxide (2) and the subsequent intramolecular nucleophilic attack [42] is more probable.
Due to the pharmacological importance of the obtained quinoline benzamides 3–4, we utilized the SwissADME website [44], which allowed us to rapidly estimate the potential biological activity of newly synthesized molecules. According to the predictions (Table S1), benzamides 3–4 displayed good drug-likeness scores with an acceptable hydrophilic-lipophilic balance (cLogP 3.89 and cLogP 3.16, respectively), obeying Lipinski’s rule [45] and presenting the optimal range for each of the six physicochemical properties (lipophilicity, size, polarity, solubility, flexibility, and saturation) in the hexagon snapshots [46]. Interestingly, introducing the benzamide fragment in the C-2 position of the quinoline ring (1) increased the lipophilicity of the comp. 3, while the second functionalization of carbon C-4 lowered it to ideal parameters (comp. 4). However, both prepared benzamides could have a good hematoencephalic barrier permeation (the total molecular polar surface area, TPSA < 140 Å2) (Table S1, Figure S18). Their ability to permeate the blood–brain barrier (BBB) is a relevant factor in drug design. Crossing the BBB easily, they could be distributed homogeneously throughout the central nervous system (CNS). It is noteworthy that, according to the SwissTargetPrediction tools [47], the most achievable macromolecular targets of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide (4) would be protein kinase inhibitors (40%) or phosphodiesterase modulators (33%), while these targets are less feasible for N-(4,7-dichloroquinolin-2-yl) benzamide (3) (33% and 13.3%, respectively) (Figure S19).

3. Materials and Methods

3.1. Chemical Analysis

The solvents and reagents were purchased from Merck and Aldrich (Merck KGaA and/or Sigma-Aldrich, Darmstadt, Germany) and employed in synthesizing the intermediate and final compounds of purity grade for synthesis. The composition and monitoring of the reactions, as well as the preliminary analysis of the purity of the synthesized compounds, were carried out by thin-layer chromatography (TLC) on Silufol UV254 plates of 0.25 mm thickness, revealed in a UV light chamber of 254 nm or an ethanolic solution of phosphomolybdic-sulfuric acids. The melting points of the products were determined in a Fisher–Johns melting point apparatus (00590Q, Thermo Scientific, Waltham, MA, USA) and the values were not corrected, reporting the average of three measurements. Infrared spectra (FT–IR) were acquired on a Thermo Scientific Nicolet iS50 FT–IR spectrophotometer (Waltham, MA, USA) with Fourier transform and an attenuated total reflectance (ATR) module (acquisition range: 4000–400 cm−1 (256 scans, resolution of 2 cm−1)).
Mass spectra were obtained in UltraScan mode using a Hitachi LaChrom Elite HPLC liquid chromatograph coupled to a Bruker Daltonics AmaZon-X mass selective detector equipped with an Apollo-type electrospray ionization or ESI (ElectroSpray Ionization) source in positive mode, and a quadrupole ion trap or QIT (Quadrupole Ion Trap) analyzer (Bruker Daltonics Inc., Bremen, Germany). The acquisition of nuclear magnetic resonance spectra 1H, 13C, and 2D variants was achieved using a Bruker Avance–400 spectrometer (Bruker, Hamburg, Germany) (400 MHz for 1H and 100 MHz for 13C) using deuterated chloroform (CDCl3, 99.8% Merck®) as the solvent. Chemical shift values (δ) are expressed in ppm. In 1H-NMR spectra, the scale was adjusted from the residual chloroform signal (7.26 ppm). Similarly, the 13C-NMR spectra were scaled from the signal characteristic for the solvent (CDCl3). The coupling constants (J) are given in Hz; the multiplicity of signals is expressed by the following abbreviations: (s) singlet, (d) doublet, (t) triplet, (dd) doublet of doublets, and (m) multiplet.

3.2. Step 1: Preparation of 4,7-Dichloroquinoline 1-oxide (2)

In a 10 mL flask, 4,7-dichloroquinoline (1) (197 mg, 1 mmol) was dissolved in chloroform (2.5 mL) and stirred for 5 min. Then, m-CPBA (206 mg, 1.2 mmol) was added gradually. It was allowed to stir for 5 h at room temperature. Once the reaction (TLC monitoring) was completed, the reaction mixture was neutralized with a NaHCO3 solution, and then the organic phase was extracted with ethyl acetate (3 × 20 mL). The resulting extracts were dried (anhydrous Na2SO4) and the solvent was removed by distillation. The crude product was purified by column chromatography with silica gel using a mixture of petroleum ether–ethyl acetate (1:3) to obtain 173 mg of N-oxide (2) as a white solid (81% yield). Rf = 0.41 (petroleum ether–ethyl acetate, 1:3); Mp. 164–166 °C; IR (ATR, νmax/cm−1): 3095 and 3043 (νArC-H), 1497–1604 (νArC=C), 1287 (νN-O), 1085 (νArC-Cl) 812–886 (νArC-H). 1H-NMR (400 MHz, CDCl3), δ(ppm): 8.79 (d, J = 1.9 Hz, 1H, 8-H), 8.43 (d, J = 6.6 Hz, 1H, 2-H), 8.16 (d, J = 9.0 Hz, 1H, 5-H), 7.70 (dd, J = 9.0, 1.95 Hz, 1H, 6-H), 7.37 (d, J = 6.55 Hz, 1H, 3-H). 13C-NMR (101 MHz, CDCl3), δ(ppm): 142.4 (8a-C), 138.3 (7-C), 135.9 (2-C), 130.8 (6-C), 129.8 (4-C), 126.8 (5-C), 126.6 (4a-C), 121.2 (3-C), 120 (8-C).

3.3. Step 2: Synthesis of N-(4,7-dichloroquinolin-2-yl) benzamide (3)

In a 10 mL crimper vial equipped with a magnetic stir, benzonitrile (0.824 mL, 8 mmol) and 97% H2SO4 (0.107 mL, 2 mmol) were added. It was left stirring for 1 min at room temperature and then N-oxide (2) (213 mg, 1 mmol) in CH2Cl2 (2 mL) was added. The reaction mixture was left stirring for 2 min at room temperature and the reaction system was sealed and heated to 70 °C in a reflux system for 24 h. TLC monitored the progress of the reaction. Once the reaction was finished, ethyl acetate (3 × 20 mL) and brine were added. The organic phase was separated and dried over anhydrous Na2SO4. The solvent was removed by distillation. The crude product was purified by column chromatography with silica gel using a mixture of petroleum ether–ethyl acetate (6:1) to obtain 291 mg of benzamide (3) as a white solid (92% yield). Rf = 0.60 (petroleum ether–ethyl acetate, 7:1); Mp. 133–134 °C; IR (ATR, νmax/cm−1): 3334 (νN-H), 2925 and 2856 (νArC-H), 1693 (νC=O), 1576–1393 (νArC=C), 1250 (νC-N), 863–759 (γArC-H). 1H-NMR (400 MHz, CDCl3), δ(ppm): 8.82 (s, 1H, N-H, -NH), 8.77 (s, 1H, 3-H), 8.11 (d, J = 9.0 Hz, 1H, 5-H), 8.00–7.97 (m, 2H, 2′-H/6′-H), 7.83 (d, J = 2.0 Hz, 1H, 8-H), 7.65–7.59 (m, 1H, 4′-H), 7.54–7.52 (m, 2H, 3′-H/5′-H), 7.49 (dd, J = 9.0, 2.0 Hz, 1H, 6-H). 13C-NMR (101 MHz, CDCl3), δ(ppm): 165.9 (C=O), 151.5 (2-C), 147.6 (8a-C), 144.5 (4-C), 137.1 (7-C), 133.6 (1′-C), 132.8 (4′-C), 129.8 (3′-C/5′-C), 127.4 (2′-C/6′-C), 127 (6-C), 126.7 (8-C), 125.7 (5-C), 123.1 (4a-C), 114.4 (3-C). COSY correlation [δH/δH]: 8.11/7.49 [5-H/6-H], 7.97/7.54 [(2′-H/6′-H)/(3′-H/5′-H)], 7.83/7.49 [8-H/6-H], 7.62/7.54 [4′-H/(3′-H/5′-H)]. HSQC correlation [δH/δC]: 8.77/114.4 [3-H/3-C], 8.11/125.7 [5-H/5-C], 7.97/127.4 [(2′-H/6′-H)/(2′-C/6′-C)], 7.83/126.7 [8-H/8-C], 7.62/132.8 [4′-H/4′-C], 7.54/129 [(3′-H/5′-H)/(3′-C/5′-C)], 7.49/127 [6-H/6-C]. HMBC correlation [δH/δC]: 8.77/123.1/144.5 [3-H/4a-C/4-C], 8.11/137.1/144.5/147.6 [5-H/7-C/4-C/8a-C], 7.97/127.4/132.8/165.9 [(2′-H/6′-H)/(2′-C/6′-C)/4′-C/0′-C], 7.83/123.1/127./137.1 [8-H/4a-C/6-C/7-C], 7.62/127.4 [4′-H/(2′-C/6′-C)], 7.54/129/133.6 [(3′-H/5′-H)/(3′-C/5′-C)/1′-C], 7.49/123.1/126.7/137.1 [6-H/4a-C/8-C/7-C]. ESI-MS, m/z (%): calculated mass for C16H11Cl2N2O2: 318.0 (37Cl), 316.0 (35Cl); found, [M+H]+: 319.0 (61%), 317.0 (100%).

3.4. Step 3: Synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl) benzamide (4)

In a 10 mL crimper vial equipped with a magnetic stir, benzamide (3) (316 mg, 1 mmol), morpholine (0.346 mL, 4 mmol), and K2CO3 (413 mg, 3 mmol) in DMF (2.5 mL) were mixed. The reaction system was sealed and heated at 120 °C for 24 h. TLC monitored the progress of the reaction. Once the reaction was finished, the reaction mixture was treated with brine. The precipitate was obtained from the mother liquor. It was filtered and dried in air to give 338 mg of the final product (4) as a very light orange solid (92% yield). Rf = 0.79 (petroleum ether–ethyl acetate, 5:1); Mp. 223–225 °C. IR (ATR, νmax/cm−1): 3279 (νN-H), 2858 and 2824 (νArC-H), 1674 (νC=O), 1577–1398 (νArC=C), 1254 (νC-N). 1H-NMR (400 MHz, CDCl3), δ(ppm): 8.76 (s, 1H, N-H), 8.17 (s, 1H, 3-H), 7.88 (d, J = 8.9 Hz, 1H, 5-H), 7.99–7.96 (m, 2H, 2′-H/6′-H), 7.78 (d, J = 2.0 Hz, 1H, 8-H), 7.62–7.57 (m, 1H, 4′-H), 7.55–7.52 (m, 2H, 3′-H/5′-H), 7.34 (dd, J = 9.0, 2.1 Hz, 1H, 6-H), 3.99 (t, J = 4.6 Hz, 4H, 2″-H/6″-H), 3.31 (t, J = 4.6 Hz, 4H, 3″-H/5″-H). 13C-NMR (101 MHz, CDCl3), δ(ppm): 166.1 (C=O), 159 (4-C), 152.8 (2-C), 148.6 (8a-C), 135.6 (7-C), 134.1 (1′-C), 132.5 (4′-C), 129 (3′-C/5′-C), 127.3 (2′-C/6′-C), 127.1 (8-C), 125.1 (5-C), 124.9 (6-C), 120 (4a-C), 101.9 (3-C), 66.8 (3″-C/5″-C), 52.7 (2″-C/6″-C). COSY correlation [δH/δH]: 7.99–7.96/7.55–7.52 [(2′-H/6′-H)/(3′-H/5′-H)], 7.88/7.34 [5-H/6-H], 7.78/7.34 [8-H/6-H], 7.62–7.57/7.55–7.52 [4′-H/(3′-H/5′-H)], 3.99/3.31 [(3″-H/5″-H)/(2″-H/6″-H)]. HSQC correlation [δH/δC]: 8.17/101.9 [3-H/3-C], 7.99–7.96/127.3 [(2′-H/6′-H)/(2′-C/6′-C)], 7.88/125.1 [5-H/5-C], 7.78/127.1 [8-H/8-C], 7.62–7.57/132.5 [4′-H/4′-C], 7.55–7.52/127.3 [(3′-H/5′-H)/(3′-C/5′-C)], 7.34/124.9 [6-H/6-C], 3.99/66.8 [(3″-H/5″-H)/(3″-C/5″-C)], 3.31/52.7 [(2″-H/6″-H)/(2″-C/6″-C)]. HMBC correlation [δH/δC]: 8.17/120 [3-H/4a-C], 7.99–7.96/127.3/132.5/166.1 [(2′-H/6′-H)/(2′-C/6′-C)/4′-C/0′-C], 7.88/135.6/148.6/159 [5-H/7-C/8a-C/4-C], 7.78/120/124.9/135.6 [8-H/4a-C/6-C/7-C], 7.62–7.57/127.3 [4′-H/(2′-C/6′-C)], 7.55–7.52/127.3/129/134.1 [(3′-H/5′-H)/(2′-C/6′-C)/(3′-C/5′-C)/1′-C], 7.49/120/127.1/135.6 [6-H/4a-C/8-C/7-C], 3.99/66.8/52.7 [(3″-H/5″-H)/(3″-C/5″-C)/(2″-C/6″-C)], 3.31/52.7/66.8 [(2″-H/6″-H)/(2″-C/6″-C)/(3″-C/5″-C)]. ESI-MS, m/z (%): calculated mass for C20H19ClN3O2: 369.1 (37Cl), 367.1 (35Cl); found, (M+H)+: 370.1 (32.6%), 368.1 (100%); 263.1 [(M+H)+-105, 94.7%].

4. Conclusions

In summary, this paper described a three-step approach for the synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide from commercially available 4,7-dichloroquinoline through the C-H amide formation of 4,7-dichloroquinoline-N-oxide. The synthetic method is simple and efficient, presenting excellent yields (77–92%). The synthesized N-(7-chloro-quinolin-2-yl)benzamides are an interesting biological model for pharmacological agent research, especially regarding antineoplastic drug design or treating central and peripheral nervous system disorders.

Supplementary Materials

The following are available online: IR spectra, ESI-MS spectra, NMR spectra and in silico prediction of physicochemical properties.

Author Contributions

D.F.A.A. and M.C.O.V. conceived the experiments; V.V.K. designed and wrote the paper. All three authors analyzed and discussed the results and data. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are contained within the article and Supplementary Materials.

Acknowledgments

The authors thank Universidad Industrial de Santander. M.C.O.V. thanks the National Program 727 for her doctoral fellowship.

Conflicts of Interest

The authors declare no conflicts of interest. The founding sponsors had no role in the study’s design, collection, analysis, or interpretation of data; the writing of the manuscript; or the decision to publish the results.

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Molbank 2024 m1796 g001
Figure 1. Representative examples of pharmacological agents based on N-(quinolinyl)amide and N-(quinolinyl)morpholine scaffolds.
Figure 1. Representative examples of pharmacological agents based on N-(quinolinyl)amide and N-(quinolinyl)morpholine scaffolds.
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Scheme 1. Synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide (4) from commercially available 4,7-dichloroquinoline (1). (a) Oxidation step: m-CPBA, CHCl3, rt, 5 h; (b) C2 functionalization (amide formation) process: Ph-C≡N, 97% H2SO4, CH2Cl2, 70 °C, 24 h; (c) C4 functionalization (amination) process: morpholine, K2CO3, DMF, 120 °C, 24 h.
Scheme 1. Synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide (4) from commercially available 4,7-dichloroquinoline (1). (a) Oxidation step: m-CPBA, CHCl3, rt, 5 h; (b) C2 functionalization (amide formation) process: Ph-C≡N, 97% H2SO4, CH2Cl2, 70 °C, 24 h; (c) C4 functionalization (amination) process: morpholine, K2CO3, DMF, 120 °C, 24 h.
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Scheme 2. Plausible reaction mechanism of C2 amide formation reaction of N-oxide 2 with PhC≡N to form N-(quinolin-2-yl) benzamide 3.
Scheme 2. Plausible reaction mechanism of C2 amide formation reaction of N-oxide 2 with PhC≡N to form N-(quinolin-2-yl) benzamide 3.
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Aparicio Acevedo, D.F.; Ortiz Villamizar, M.C.; Kouznetsov, V.V. Three-Step Synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide from 4,7-Dichloroquinoline. Molbank 2024, 2024, M1796. https://doi.org/10.3390/M1796

AMA Style

Aparicio Acevedo DF, Ortiz Villamizar MC, Kouznetsov VV. Three-Step Synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide from 4,7-Dichloroquinoline. Molbank. 2024; 2024(1):M1796. https://doi.org/10.3390/M1796

Chicago/Turabian Style

Aparicio Acevedo, Deiby F., Marlyn C. Ortiz Villamizar, and Vladimir V. Kouznetsov. 2024. "Three-Step Synthesis of N-(7-chloro-4-morpholinoquinolin-2-yl)benzamide from 4,7-Dichloroquinoline" Molbank 2024, no. 1: M1796. https://doi.org/10.3390/M1796

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