Diversity in Cell Morphology, Composition, and Function among Adipose Depots in River Buffaloes
Abstract
:1. Introduction
2. Results
2.1. Cellular Morphology
2.2. Triglyceride Content and Crude Fat Content
2.3. Fatty Acid Composition
2.4. Overview of the RNA Sequencing
2.5. Functional Divergence between Subcutaneous and Visceral Depots
2.6. WGCNA Reveals the Functional Features of SSAT and PRAT Depots
2.7. Screening and Expression of Core Genes
3. Discussion
3.1. SAT Depots Have a Larger Cell Volume Than VAT Depots
3.2. SSAT Is Significantly Different from ATs in Other Depots
3.3. PRAT Is Active in FA Oxidation for Energy Supply
3.4. Characterizing Adipose Tissue into Subcutaneous Fat and Visceral Fat Is Not Enough
4. Materials and Methods
4.1. Sample Preparation
4.2. Histology of Adipose Tissue
4.3. Triglyceride Measurements
4.4. Fatty Acid Analysis
4.5. RNA Isolation and Sequencing
4.6. Transcriptome Assembly and mRNA Expression Analysis
4.7. Differential Expression Analysis
4.8. Weighted Gene Co-Expression Network Analysis
4.9. Functional Enrichment Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yang, X.; Zhu, R.; Song, Z.; Shi, D.; Huang, J. Diversity in Cell Morphology, Composition, and Function among Adipose Depots in River Buffaloes. Int. J. Mol. Sci. 2023, 24, 8410. https://doi.org/10.3390/ijms24098410
Yang X, Zhu R, Song Z, Shi D, Huang J. Diversity in Cell Morphology, Composition, and Function among Adipose Depots in River Buffaloes. International Journal of Molecular Sciences. 2023; 24(9):8410. https://doi.org/10.3390/ijms24098410
Chicago/Turabian StyleYang, Xintong, Ruirui Zhu, Ziyi Song, Deshun Shi, and Jieping Huang. 2023. "Diversity in Cell Morphology, Composition, and Function among Adipose Depots in River Buffaloes" International Journal of Molecular Sciences 24, no. 9: 8410. https://doi.org/10.3390/ijms24098410