Interplay between Inter-Subunit Rotation of the Ribosome and Binding of Translational GTPases

Round 1

Reviewer 1 Report

Comments for author File: Comments.pdf

Author Response

We are glad that both reviewers found our manuscript interesting and worthy publishing in IJMS. We have fixed all the typos found by reviewers. Following their advice, we also added new Figure 5 that summarizes our findings.

We hope that our manuscript is now ready for publication.

Reviewer 2 Report

The current manuscript by Das, Adiletta and Ermolenko addresses a question of how the binding of ribosome GTPases LepA and EF-G affects the equilibrium between the none-rotated (NR) and (rotated) R conformations of the ribosome   More specifically, using smFRET the authors study how the binding of translational GTPases affects intersubunit rotation of the ribosome. Undoubtedly, this topic is extremely important  because it is directly related to deciphering of the mechanism of the ribosome working as a molecular maсhine.  In the case of EF-G, a lot of data has been previously accumulated in the literature on its interaction with the ribosome during translocation So, the authors in fact convincingly and elegantly proved  the widely accepted model by Rodnina et al. that required additional evidence. However, in the case of LepA, such data has not been previously obtained. Overall, this work is a very well done and presented as a very clearly written manuscript. The results of this work will be interesting not only for ribosomologists but to a broad “readership”.

 

Author Response

We are glad that both reviewers found our manuscript interesting and worthy publishing in IJMS. We have fixed all the typos found by the reviewers. Following their advice, we also added new Figure 5 that summarizes our findings.

We hope that our manuscript is now ready for publication.