Phosphate (Pi) Transporter PIT1 Induces Pi Starvation in Salmonella-Containing Vacuole in HeLa Cells
Abstract
:1. Introduction
2. Results
2.1. S. Typhimurium Infection Induces PIT1 Expression in HeLa Cells
2.2. PIT1 Affects S. Typhimurium Intracellular Replication
2.3. PIT1 Transports Pi into Cytosol and Regulates SPI-2 Genes via PhoBR
2.4. Upregulated PIT1 Prevents the Fusion of SCVs with Lysosomes
2.5. LPS of S. Typhimurium Triggers PIT1 in HeLa Cells
2.6. S. Typhimurium Exploits TLR4-MyD88-NF-kB Pathway to Regulate PIT1
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. Antibodies
4.3. Cell Culture and Transfection
4.4. RNA Extraction and qRT-PCR
4.5. Quantitative PCR Analysis
4.6. Bacterial Infection and Replication Assays
4.7. Immunofluorescence Analysis
4.8. Isolation of Intracellular SCVs
4.9. Western Blotting Analysis
4.10. Determination of the Phosphate Concentration in SCVs
4.11. LPS Stimulation
4.12. Quantification and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yang, W.; Feng, Y.; Yan, J.; Kang, C.; Yao, T.; Sun, H.; Cheng, Z. Phosphate (Pi) Transporter PIT1 Induces Pi Starvation in Salmonella-Containing Vacuole in HeLa Cells. Int. J. Mol. Sci. 2023, 24, 17216. https://doi.org/10.3390/ijms242417216
Yang W, Feng Y, Yan J, Kang C, Yao T, Sun H, Cheng Z. Phosphate (Pi) Transporter PIT1 Induces Pi Starvation in Salmonella-Containing Vacuole in HeLa Cells. International Journal of Molecular Sciences. 2023; 24(24):17216. https://doi.org/10.3390/ijms242417216
Chicago/Turabian StyleYang, Wen, Yingxing Feng, Jun Yan, Chenbo Kang, Ting Yao, Hongmin Sun, and Zhihui Cheng. 2023. "Phosphate (Pi) Transporter PIT1 Induces Pi Starvation in Salmonella-Containing Vacuole in HeLa Cells" International Journal of Molecular Sciences 24, no. 24: 17216. https://doi.org/10.3390/ijms242417216