IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish
Abstract
:1. Introduction
2. Results
2.1. Generation of il-6−/− Zebrafish
2.2. Effect of il-6 Mutation on Liver Injury Caused by A. hydrophila
2.3. Identification of DEGs in the Liver Transcriptome of WT and il-6−/− Zebrafish after A. hydrophila Infection
2.4. Effect of il-6 Mutation on Oxidative Stress Caused by A. hydrophila in the Liver
2.5. Expression and Purification of Recombinant Zebrafish IL-6 (rDrIL-6)
2.6. Effect of rDrIL-6 on A. hydrophila-Induced Liver Injury in il-6−/− Zebrafish
3. Discussion
4. Materials and Methods
4.1. Zebrafish Strain
4.2. Generation of il-6−/− Zebrafish by CRISPR/Cas9
4.3. A. hydrophila Infection and Samples Collection
4.4. RNA Extraction, Library Construction, and RNA-Sequencing
4.5. RNA-Seq Data Analysis
4.6. Fluorescent Quantitative Real-Time PCR (qPCR)
4.7. Protein Expression and Purification
4.8. HE Staining
4.9. ROS Staining
4.10. Measurement of Liver Biochemical Indicators
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhai, W.; Wang, Z.; Ye, C.; Ke, L.; Wang, H.; Liu, H. IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish. Int. J. Mol. Sci. 2023, 24, 17215. https://doi.org/10.3390/ijms242417215
Zhai W, Wang Z, Ye C, Ke L, Wang H, Liu H. IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish. International Journal of Molecular Sciences. 2023; 24(24):17215. https://doi.org/10.3390/ijms242417215
Chicago/Turabian StyleZhai, Wenya, Zhensheng Wang, Canxun Ye, Lan Ke, Huanling Wang, and Hong Liu. 2023. "IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish" International Journal of Molecular Sciences 24, no. 24: 17215. https://doi.org/10.3390/ijms242417215