Synergistic Therapeutic Potential of Dual 3D Mesenchymal Stem Cell Therapy in an Ischemic Hind Limb Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Cell Morphology and Angiogenic Property of 2D or Dual 3D Adipose-Derived Mesenchymal Stem Cells (ASCs)
2.2. Secretions from Dual ASC-3D and ASC-3Dc (D-3D) Enhance Cell Migration and Tubular Network Formation
2.3. D-3D Shows Robust Therapeutic Potential in Hind Limb Ischemia (HLI)
2.4. D-3D Injection Enhances Capillary Density in a Hind Limb Mouse Model
2.5. 3D ASCs Differentiated into Endothelial Cells In Vivo
3. Discussion
4. Materials and Methods
4.1. 2D Culture
4.2. Culture with a Cytokine Cocktail
4.3. 3D Culture
4.4. Suspension Cell Culture and Conditioned Medium (CM) Collection
4.5. Quantitative Reverse Transcription (qRT)–Polymerase Chain Reaction (PCR) Analysis
4.6. Matrigel Tube Formation Assay
4.7. Scratch Migration Assay
4.8. Matrigel Plug Assay
4.9. Cell Transplantation in the HLI Mouse Model
4.10. Histological Analysis
4.11. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Human | Mouse |
---|---|---|
VEGF-A | Hs99999070_m1 | Mm01204733_m1 |
FGF-2 | Hs00266645_m1 | Mm00433287_m1 |
HGF | Hs00300159_m1 | Mm01135184_m1 |
IGF-1 | Hs01547657-m1 | Mm00439560_m1 |
GAPDH | Hs99999905_m1 | Mm99999915_g1 |
SDF-1 | Hs00171022_m1 | |
GCP-2 | Hs00237017_m1 |
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Chae, D.-S.; An, S.J.; Han, S.; Kim, S.-W. Synergistic Therapeutic Potential of Dual 3D Mesenchymal Stem Cell Therapy in an Ischemic Hind Limb Mouse Model. Int. J. Mol. Sci. 2023, 24, 14620. https://doi.org/10.3390/ijms241914620
Chae D-S, An SJ, Han S, Kim S-W. Synergistic Therapeutic Potential of Dual 3D Mesenchymal Stem Cell Therapy in an Ischemic Hind Limb Mouse Model. International Journal of Molecular Sciences. 2023; 24(19):14620. https://doi.org/10.3390/ijms241914620
Chicago/Turabian StyleChae, Dong-Sik, Sang Joon An, Seongho Han, and Sung-Whan Kim. 2023. "Synergistic Therapeutic Potential of Dual 3D Mesenchymal Stem Cell Therapy in an Ischemic Hind Limb Mouse Model" International Journal of Molecular Sciences 24, no. 19: 14620. https://doi.org/10.3390/ijms241914620