A Rapid and Specific Real-Time PCR Assay for the Detection of Clinically Relevant Mucorales Species
Abstract
:1. Introduction
2. Results
2.1. Samples Analyzed
2.2. PCR Performance
2.3. Applicability of the qPCR Method
3. Discussion
4. Materials and Methods
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Standard Plasmid DNA | Intra-Assay Variability (%) | Inter-Assay Variability (%) |
---|---|---|
102 | 0.473 | 0.785 |
103 | 0.671 | 1.699 |
104 | 0.633 | 1.345 |
105 | 0.088 | 0.888 |
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Bergallo, M.; Tullio, V.; Roana, J.; Allizond, V.; Mandras, N.; Daprà, V.; Dini, M.; Comini, S.; Cavallo, L.; Gambarino, S.; et al. A Rapid and Specific Real-Time PCR Assay for the Detection of Clinically Relevant Mucorales Species. Int. J. Mol. Sci. 2022, 23, 15066. https://doi.org/10.3390/ijms232315066
Bergallo M, Tullio V, Roana J, Allizond V, Mandras N, Daprà V, Dini M, Comini S, Cavallo L, Gambarino S, et al. A Rapid and Specific Real-Time PCR Assay for the Detection of Clinically Relevant Mucorales Species. International Journal of Molecular Sciences. 2022; 23(23):15066. https://doi.org/10.3390/ijms232315066
Chicago/Turabian StyleBergallo, Massimiliano, Vivian Tullio, Janira Roana, Valeria Allizond, Narcisa Mandras, Valentina Daprà, Maddalena Dini, Sara Comini, Lorenza Cavallo, Stefano Gambarino, and et al. 2022. "A Rapid and Specific Real-Time PCR Assay for the Detection of Clinically Relevant Mucorales Species" International Journal of Molecular Sciences 23, no. 23: 15066. https://doi.org/10.3390/ijms232315066