COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells
Abstract
:1. Introduction
2. Results
2.1. COP1 Is Required for Dark-Induced Stomatal Closure
2.2. COP1 Acts Downstream of H2O2 in Dark-Induced Stomatal Closure
2.3. COP1 Acts Upstream of NO in Dark-Induced Stomatal Closure
2.4. Expression of FT, TSF and SOC1 Is Downregulated by Darkness via the COP1-Dependent Manner
2.5. FT, TSF and SOC1 Participate in Dark-Induced Stomatal Closure
2.6. Mutation of FT, TSF and SOC1 Accelerates Dark-Induced NO but Not H2O2 Accumulation in Guard Cells
3. Discussion
3.1. COP1 Mediates Dark-Induced Stomatal Closure through Promotion of NO Accumulation
3.2. Floral Integrators FT, TSF and SOC1 Negatively Regulate Dark-Induced Stomatal Closure
3.3. COP1 Promotes NO Accumulation by Suppressing FT, TSF and SOC1 Expression
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Stomatal Bioassay
4.3. Measurement of Endogenous H2O2 and NO in Guard Cells
4.4. Gene Expression Analysis
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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An, Y.-Y.; Li, J.; Feng, Y.-X.; Sun, Z.-M.; Li, Z.-Q.; Wang, X.-T.; Zhang, M.-X.; He, J.-M. COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells. Int. J. Mol. Sci. 2022, 23, 15037. https://doi.org/10.3390/ijms232315037
An Y-Y, Li J, Feng Y-X, Sun Z-M, Li Z-Q, Wang X-T, Zhang M-X, He J-M. COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells. International Journal of Molecular Sciences. 2022; 23(23):15037. https://doi.org/10.3390/ijms232315037
Chicago/Turabian StyleAn, Yu-Yan, Jing Li, Yu-Xin Feng, Zhi-Mao Sun, Zhong-Qi Li, Xiao-Ting Wang, Mei-Xiang Zhang, and Jun-Min He. 2022. "COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells" International Journal of Molecular Sciences 23, no. 23: 15037. https://doi.org/10.3390/ijms232315037